Pyoverdin‐facilitated iron uptake in <i>Pseudomonas aeruginosa</i>: immunological characterization of the ferripyoverdin receptor

Meyer, Jean Marie; Hohnadel, Dany; Khan, Asad; Cornélis, Pierre

doi:10.1111/j.1365-2958.1990.tb00719.x

Cited by 59 publications

(40 citation statements)

References 22 publications

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“…As previously shown for P. aeruginosa ATCC 15692 and ATCC 27853, this procedure allows recognition of strains having a PVD-mediated iron uptake system identical to the ones of the two typestrains (Cornelis et al, 1989a) by specifically revealing the ferripyoverdine outer-membrane receptor as demonstrated for P. aeruginosa ATCC 15692 (Meyer et al, 1990). The anti-P. aeruginosa Pa6-IROMP antiserum behaved the same, reacting strongly with the 80 kDa IROMP of strain Pa6, while it cross-reacted only slightly with one or two outer-membrane- shown in Table 3.…”

Section: Siderotyping By Ferripyoverdine Outer-membrane Receptor Immumentioning

confidence: 80%

“…The purification of bacterial outer membranes and visualization of the iron-regulated outermembrane proteins (IROMPs) be denaturating PAGE, the preparation of crude and partially purified rabbit polyclonal antisera raised against the main IROMP of P. aeruginosa strains and the immunodetection of the IROMPs by Western blotting have been described previously (Cornelis et al, 1989a ;Meyer et al, 1990). The large number of strains investigated in the present study required a rapid and easy method of outer 1983CIP 1994CIP 1983CIP 1983CIP 1983CIP 1983CIP 1994(ATCC 23268) CIP 1994(ATCC 23267) CIP 1983CIP 1983CIP 1994CIP 1994CIP 1994CIP 1983CIP 1983CIP 1983CIP 1983CIP 1983CIP 1983CIP 1983CIP 1994CIP 1983CIP 1983CIP 1983CIP 1983CIP 1983CIP 1983CIP 1983CIP 1983 t As indicated in the catalogues or in the literature; -, no information available.…”

Section: Methodsmentioning

confidence: 99%

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Use of Siderophores to Type Pseudomonads: The Three Pseudomonas Aeruginosa Pyoverdine Systems

et al. 1997

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Eighty-eight Pseudomonas aenrginosa isolates, most of them from the Collection of Bacterial Strains of the lnstitut Pasteur, Paris, were analysed for their pyoverdine-mediated iron incorporation system by different methods, including pyoverdine isoelectrofocusing analysis, pyoverdine-mediated growth stimulation, immunoblot detection of (ferri)pyoverdine outer-membrane receptor and pyoverdine-facilitated iron uptake. The same grouping of the strains was reached by each of these methods, resulting in the classification of the P. aenrginosa isolates, even those which were devoid of pyoverdine production, into three different siderophore types. Forty-two percent of the strains were identified with the --strain P. aeruginosa ATCC 15692 (group I), 42% were identical with the second type-strain P. aenrginosa ATCC 27853 (group II) and 16% reacted identically with the clinical isolate P. aenrginosa Pa6, whose pyoverdine was recognized in this study to be identical in structure to the pyoverdine produced by a natural isolate, P. aeruginosa strain R. No new pyoverdine species was detected among these strains.

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Section: Siderotyping By Ferripyoverdine Outer-membrane Receptor Immumentioning

confidence: 80%

Section: Methodsmentioning

confidence: 99%

Use of Siderophores to Type Pseudomonads: The Three Pseudomonas Aeruginosa Pyoverdine Systems

et al. 1997

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“…aeruginosa strains, Cornelis et al (1989) noted three pyoverdin uptake groups and polyclonal antisera raised against an 80 kDa IROMP reacted only against strains producing the same siderophore. This antiserum was subsequently shown to inhibit ferri-pyoverdin transport in this strain, confirming that the 80 kDa IROMP was a transporter (Meyer et al, 1990). Poole et al (1991) have identified a 90 kDa IROMP ferri-pyoverdin transporter in the pyoverdin-deficient strain 6609.…”

Section: Introductionmentioning

confidence: 78%

Siderophore-specific induction of iron uptake in Pseudomonas aeruginosa

Gensberg

Hughes²,

Smith³

1992

Journal of General Microbiology

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Pseudomonas aeruginosa has two siderophore-based high-affinity iron-uptake systems utilizing pyoverdin and pyochelin. Using strain IAl, a mutant deficient in production of both siderophores, we have shown that addition of purified siderophore to the growth medium induces expression of specific iron-regulated outer-membrane proteins and increases 55Fe-siderophore transport. Addition of pyoverdin from the parent strain P A 0 1 or from a clinical strain 0:12 induced expression of an 85 kDa IROMP and increased the rate of 55Fe-pyoverdin transport.Transport rates for 55Fe-PA01 pyoverdin increased from 1.27 to 3.57 pmol Fe min-l per lo9 cells. Addition of purified pyochelin induced expression of a 75 kDa IROMP accompanied with increased 55Fe-pyochelin uptake without affecting 55Fe-pyoverdin transport. 55Fe-pyochelin transport increased from 0.3 to 10.6 pmol min-' per lo9 cells. Addition of pyoverdin from the parent strain or a chromatographically distinct pyoverdin caused increased reactivity with an anti-85 kDa mAb in Western blotting, indicating that the same receptor is being induced. These results suggest that P. aeruginosa can respond specifically to the presence of siderophore and moreover that not only can the pyoverdin receptor transport its cognate ferri-pyoverdin but also different ferripyoverdms, albeit at a reduced rate.

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“…The purification procedure for pyoverdines and electrophoretic analysis were as described previously (Meyer & Abdallah, 1978). The protocol used for iron uptake experiments has been described elsewhere (Meyer et a/., 1990). UV-visible spectra were obtained from a Uvikon 930 (Kontron) spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

Novel pyoverdine biosynthesis gene(s) of Pseudomonas aeruginosa PAO

et al. 1996

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~~ ~Conjugational mobilization of a Pseudomonas aeruginosa PAOl cosmid bank (in pMMB33) into a pyoverdine-def icient (pvd) mutant harbouring a mutation in the 47 min region of the chromosome yielded one clone which restored yellow-green pigmentation and fluorescence when grown on iron-def icient medium. The relevant pMMB33-derivative cosmid, pPYP17, contained a 151 kb insert which was subcloned into pKT240 as a 10.8 kb Sacl-Clal fragment conferring the same phenotype. This derivative, pPYPl80, like pPYP17, also conferred an apparent wild-type phenotype on pvd mutants previously shown to map genetically in the 23 min region of the P. aeruginosa PA0 chromosome. Physical mapping indicated that the cloned DNA fragment is located at the 66-70 min region of the PA0 chromosome, demonstrating that the restored apparent wild-type phenotype observed for the transconjugants was not the result of a true gene complementation. A gene interruption was obtained by replacing a 0 6 kb BgllI-Bglll region of pPYP180 necessary for the expression of the pigmentation/fluorescence phenotype, by a Hgr interposon (OHg). After conjugational transfer and introduction of the mutagenized fragment into the PAOl chromosome by gene replacement, pyoverdine-def icient mutants were recovered, indicating that the fragment indeed contained at least one gene involved in pyoverdine synthesis. The yellow-green fluorescent compound produced by such cells harbouring plasmids pPYP17 or pPYP180 differed from pyoverdine in several aspects and was consequently named pseudoverdine. Although pseudoverdine was able t o complex iron, it was unable t o restore growth to pvd mutants in the presence of the iron chelator ethylenediamine di(o-hydroxyphenylacetic acid), or to mediate iron uptake into PAOl .Pseudoverdine lacked a peptide chain but possessed spectral properties similar t o pyoverdine, suggesting that it was structurally related to the chromophore of the pyoverdine molecule. The recent structural determination of pseudoverdine as a coumarin derivative confirmed this view and sheds some light on the biosynthetic pathway of the pyoverdine chromophore.

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Pyoverdin‐facilitated iron uptake in Pseudomonas aeruginosa: immunological characterization of the ferripyoverdin receptor

Cited by 59 publications

References 22 publications

Use of Siderophores to Type Pseudomonads: The Three Pseudomonas Aeruginosa Pyoverdine Systems

Use of Siderophores to Type Pseudomonads: The Three Pseudomonas Aeruginosa Pyoverdine Systems

Siderophore-specific induction of iron uptake in Pseudomonas aeruginosa

Novel pyoverdine biosynthesis gene(s) of Pseudomonas aeruginosa PAO

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