Pyoverdine type siderophores from pseudonomas fluorescens

Poppe, K.J.; Taraz, K.; Budzikiewicz, H.

doi:10.1016/s0040-4020(01)86810-1

Cited by 45 publications

(37 citation statements)

References 26 publications

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“…This is not surprising since it is well established that vanadium can be liganded by hydroxamates, carboxylates and catecholates (Keller et al, 1991 ;Rehder, 1991). Others have also demonstrated the fact that PVD, as well as PCH, can complex copper, zinc or manganese (Poppe et al, 1987 ;Chen et al, 1994 ;Visca et al, 1992 ;Bouby et al, 1999). Visca et al (1992) suggested that PCH could play a role in the acquisition of metals other than Fe(III), such as Co(II) and Mo(VI).…”

Section: Discussionmentioning

confidence: 96%

Vanadium interferes with siderophore-mediated iron uptake in Pseudomonas aeruginosa

et al. 2000

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Vanadium is a metal that under physiological conditions can exist in two oxidation states, V(IV) (vanadyl ion) and V(V) (vanadate ion).Here, it was demonstrated that both ions can form complexes with siderophores. Pseudomonas aeruginosa produces two siderophores under iron-limiting conditions, pyoverdine (PVD) and pyochelin (PCH). Vanadyl sulfate, at a concentration of 1-2 mM, strongly inhibited growth of P. aeruginosa PAO1, especially under conditions of severe iron limitation imposed by the presence of non-utilizable Fe(III) chelators. PVD-deficient mutants were more sensitive to vanadium than the wild-type, but addition of PVD did not stimulate their growth. Conversely, PCH-negative mutants were more resistant to vanadium than the wild-type strain. Both siderophores could bind and form complexes with vanadium after incubation with vanadyl sulfate (1 :1, in the case of PVD ; :1, in the case of PCH). Although only one complex with PVD, V(IV)-PVD, was found, both V(IV)-and V(V)-PCH were detected. V-PCH, but not V-PVD, causedstrong growth reduction, resulting in a prolonged lag phase. Exposure of PAO1 cells to vanadium induced resistance to the superoxide-generating compound paraquat, and conversely, exposure to paraquat increased resistance to V(IV). Superoxide dismutase (SOD) activity of cells grown in the presence of V(IV) was augmented by a factor of two. Mutants deficient in the production of Fe-SOD (SodB) were particularly sensitive to vanadium, whilst sodA mutants deficient for Mn-SOD were only marginally affected. In conclusion, it is suggested that V-PCH catalyses a Fenton-type reaction whereby the toxic superoxide anion O N 2 is generated, and that vanadium compromises PVD utilization.

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Section: Discussionmentioning

confidence: 96%

Vanadium interferes with siderophore-mediated iron uptake in Pseudomonas aeruginosa

et al. 2000

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“…Because of its high Fe(III)-binding constant (8), it might be useful in competition with other microorganisms (8,9,33) under nutritionally poor conditions. Many pyoverdins produced by saprophytic fluorescent Pseudomonas strains have been described, and their spectral characteristics are similar whatever peptide chain or dicarboxylic acid is connected to the chromophore (1,2,24,29). This is due to the quinoline chromophore found in all pyoverdins (2).…”

Section: Discussionmentioning

confidence: 98%

Production and Comparison of Peptide Siderophores from Strains of Distantly Related Pathovars of Pseudomonas syringae and Pseudomonas viridiflava LMG 2352

Bultreys

Gheysen

2000

Appl Environ Microbiol

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The production of peptide siderophores and the variation in siderophore production among strains of Pseudomonas syringae and Pseudomonas viridiflava were investigated. An antibiose test was used to select a free amino acid-containing agar medium favorable for production of fluorescent siderophores by two P. syringae strains. A culture technique in which both liquid and solid asparagine-containing culture media were used proved to be reproducible and highly effective for inducing production of siderophores in a liquid medium by the fluorescent Pseudomonas strains investigated. Using asparagine as a carbon source appeared to favor siderophore production, and relatively high levels of siderophores were produced when certain amino acids were used as the sole carbon and energy sources. Purified chelated siderophores of strains of P. syringae pv. syringae, P. syringae pv. aptata, P. syringae pv. morsprunorum, P. syringae pv. tomato, and P. viridiflava had the same amino acid composition and spectral characteristics and were indiscriminately used by these strains. In addition, nonfluorescent strains of P. syringae pv. aptata and P. syringae pv. morsprunorum were able to use the siderophores in biological tests. Our results confirmed the proximity of P. syringae and P. viridiflava; siderotyping between pathovars of P. syringae was not possible. We found that the spectral characteristics of the chelated peptide siderophores were different from the spectral characteristics of typical pyoverdins. Our results are discussed in relation to the ecology of the organisms and the conditions encountered on plant surfaces.The species Pseudomonas syringae contains all of the phytopathogenic and oxidase-negative fluorescent pseudomonads except Pseudomonas viridiflava (27,38). P. syringae is divided into 57 pathovars that are pathogenic for numerous monocot and dicot crops (13). P. syringae strains are well adapted to conditions on plant surfaces. A better understanding of the ecological benefits of these pathogens is necessary if new and efficient methods of biological control are to be developed. One of these benefits could be the production of peptide siderophores in iron-deficient environments (8,33). In general, the peptide siderophores produced by fluorescent Pseudomonas strains are pyoverdins (2, 3). All pyoverdins contain the same quinoline chromophore, a peptide chain, and a dicarboxylic acid (or the corresponding amide) connected to the chromophore. The peptide chain is always the same for a given strain but is different in different strains and species (2). Two partially characterized peptide siderophores of P. syringae that have been described (8, 33) have Fe(III)-binding constants at pH 7.0 of about 1 ϫ 10 25 . These values are 10 times higher than the values obtained for pyoverdins produced by saprophytic fluorescent Pseudomonas strains (8, 33). Therefore, it would be interesting to know whether production of these molecules is common in P. syringae strains and whether the molecules are effectively produced on plant surf...

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“…[2] and [4]) gave the following picture: Fe3+-2 is neutral be tween pH 6.5 and 3.0 while Fe3+-3 carries a single negative charge at pH 6.5 (mobility 0.21 deter mined [13] relative to aspartic acid) stemming from a COOH-group with pKc/ between 4 and 5 (almost complete protonation at pH 3). This behavior is paralleled by the free ligands 2 and 3 which show a somewhat higher mobility in toto caused by the presence of the uncomplexed OH-groups (Fe3+ being replaced by 4 H +).…”

Section: Characterization Of the Pyoverdins 2 Andmentioning

confidence: 99%

New Pyoverdin-Type Siderophores from Pseudomonas fluorescens

Mohn¹,

Taraz²,

Budzikiewicz³

1990

Zeitschrift Für Naturforschung B

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A series o f Pseudomonas species -the so-called fluorescent group -produces peptidic sidero phores when grown in an iron-deficient medium. They all contain (1 S)-5-amino-2,3-dihydro-8,9-dihydroxy-1 H-pyrimido[ 1,2-a]quinoline-1 -carboxylic acid (la) [3] or its dihydro derivative as a chrom o phore. The amino group is amidically bound to a small dicarboxylic acid (usually several pyoverdins differing only in the nature of the latter can be found to co-occur) while the carboxylic group is linked to a peptide residue (not necessarily via its N-terminus). Pyoverdins produced by the various Pseudomonas species or even subspecies usually differ in the peptidic part of the molecule. By now the isolation o f about 20 pyoverdins with different peptidic portions has been described [5], but for only six of them (not counting variations in the na ture of the dicarboxylic acid) the structure has been determined by spectroscopic and chemical methods (three from Ps. sp. [6][7][8]

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Pyoverdine type siderophores from pseudonomas fluorescens

Cited by 45 publications

References 26 publications

Vanadium interferes with siderophore-mediated iron uptake in Pseudomonas aeruginosa

Vanadium interferes with siderophore-mediated iron uptake in Pseudomonas aeruginosa

Production and Comparison of Peptide Siderophores from Strains of Distantly Related Pathovars of Pseudomonas syringae and Pseudomonas viridiflava LMG 2352

New Pyoverdin-Type Siderophores from Pseudomonas fluorescens

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