Pyramiding of Ryd2 and Ryd3 conferring tolerance to a German isolate of Barley yellow dwarf virus-PAV (BYDV-PAV-ASL-1) leads to quantitative resistance against this isolate

Abstract

Barley yellow dwarf virus (BYDV) is an economically important pathogen of barley, which may become even more important due to global warming. In barley, several loci conferring tolerance to BYDV-PAV-ASL-1 are known, e.g. Ryd2, Ryd3 and a quantitative trait locus (QTL) on chromosome 2H. The aim of the present study was to get information whether the level of tolerance against this isolate of BYDV in barley can be improved by combining these loci. Therefore, a winter and a spring barley population of doubled haploid (DH) lines were genotyped by molecular markers for the presence of the susceptibility or the resistance encoding allele at respective loci (Ryd2, Ryd3, QTL on chromosome 2H) and were tested for their level of BYDV-tolerance after inoculation with viruliferous (BYDV-PAV-ASL-1) aphids in Weld trials. In DH-lines carrying the combination Ryd2 and Ryd3, a signiWcant reduction of the virus titre was detected as compared to lines carrying only one of these genes. Furthermore, spring barley DH-lines with this allele combination also showed a signiWcantly higher relative grain yield as compared to lines carrying only Ryd2 or Ryd3. The QTL on chromosome 2H had only a small eVect on the level of tolerance in those lines carrying only Ryd2, or Ryd3 or a combination of both, but the eVect in comparison to lines carrying no tolerance allele was signiWcant. Overall, these results show that the combination of Ryd2 and Ryd3 leads to quantitative resistance against BYDV-PAV instead of tolerance.

Effects of n-butanol on barley microspore embryogenesis

“…Additionally, Barley DHs have been of great value to speed up the development of mapping populations for marker-trait associations and comparative genomics and map-based cloning Pickering 2005, Forster et al 2007). Recently DHs have played a fundamental role in the application of high-throughput genotyping systems in the construction of barley consensus maps (Alsop et al 2010, Muñoz-Amatriaín et al 2011, Poland et al 2012, and use of marker assisted selection (MAS) systems (Melchinger et al 2011) especially for resistance to disease and abiotic stress (Riedel et al 2011, Castro et al 2012, Fisk et al 2013). …”

Mapping of QTL for Tolerance to Cereal Yellow Dwarf Virus in Two‐rowed Spring Barley

“…Ryd3 has been located on chromosome 6H (Niks et al, 2004). Riedel et al (2011) reported that pyramiding these two genes in doubled haploid lines resulted in a significant reduction of virus titer, or quantitative resistance to BYDV‐PAV. Ryd4 Hb , on chromosome 3HL, has been reported as contributing complete resistance to BYDV.…”

“…Whereas tolerance to BYDV‐PAV has been well studied (Riedel et al, 2011) little is known about tolerance to CYDV. Sources of tolerance have been identified but no information on their inheritance is available (Capettini et al, 2002).…”

Exploiting intra-cultivar variation to select forBarley yellow dwarf virus-PAV(BYDV-PAV) resistance in barley

“…BYDVs induce yield losses ranging from 5% to 80%, with an average of 30% in affected fields (Perry et al 2000). Tolerance to BYDV-PAV has been well-studied (Riedel et al 2011). Several genes have been reported as contributing tolerance/resistance to BYDV: ryd1, a recessive gene identified in the spring barley cultivar 'Rojo' (Suneson 1955); Ryd2 and Ryd3, identified in Ethiopian landraces (Schaller et al 1964;Niks et al 2004).…”