Pyramiding taro cystatin and fungal chitinase genes driven by a synthetic promoter enhances resistance in tomato to root-knot nematode Meloidogyne incognita

Chan, Yuan-Li; He, Yong; Hsiao, Tsen-Tsz; Wang, Chii-Jeng; Zhi-hong, Tian; Yeh, Kai‐Wun

doi:10.1016/j.plantsci.2014.11.010

Cited by 32 publications

(21 citation statements)

References 35 publications

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“…In case of PPNs, these PIs become active against all the four classes of proteinases from nematodes, i.e., serine, cysteine, metalloproteinases, and aspartic. The PIs used for nematode resistance studies are CpTI (Hepher and Atkinson, 1992), sweet potato ( Ipomoea batatas ) serine PI (sporamin or SpTI-1) (Cai et al, 2003), PIN2 (Vishnudasan et al, 2005), rice ( Oryza sativa ) cystatin (Oc-IΔD86) (Urwin et al, 1997, 1998), and some others cystatins from maize ( Zea mays ), taro ( Colocasia esculenta ), and sunflower ( Helianthus annuus ) (Fuller et al, 2008; Chan et al, 2015). …”

Section: Use Of Proteinase Inhibitor Coding Genesmentioning

confidence: 99%

“…As compared to other proteinase inhibitors, cystatins from different plant species have met the promise of enhancement of nematode resistance in a variety of crop plants (Urwin et al, 1997, 1998; Chan et al, 2010, 2015; Green et al, 2012; Tripathi et al, 2015; Papolu et al, 2016). Heterologous expression of a taro cystatin established considerable degree of resistance in tomato against M. incognita infection by interference with nematode ability of sex determination and gall formation (Chan et al, 2010).…”

Section: Use Of Proteinase Inhibitor Coding Genesmentioning

confidence: 99%

“…Moreover, transgenic expression of proteinase inhibitor from maize and synthetic chemodisruptive peptide resulted in enhance yield and nematode resistance in plantain. Similarly, dual overexpression a taro cysteine proteinase inhibitor (CeCPI) and a fungal chitinase (PjCHI-1) in tomato under the control of a synthetic promoter, pMSPOA, (with NOS-like and SP8a elements), had negative effects on reproduction of M. incognita (Chan et al, 2015). This study further revealed that dual gene transformation had more inhibition of nematodes than plants transformed with a single gene.…”

Section: Use Of Proteinase Inhibitor Coding Genesmentioning

confidence: 99%

“…This study further revealed that dual gene transformation had more inhibition of nematodes than plants transformed with a single gene. This advocated that the use of gene pyramiding could be employed for developing and improving nematode resistance in plants (Chan et al, 2015; Tripathi et al, 2015, 2017). …”

Section: Use Of Proteinase Inhibitor Coding Genesmentioning

confidence: 99%

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Transgenic Strategies for Enhancement of Nematode Resistance in Plants

et al. 2017

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Plant parasitic nematodes (PPNs) are obligate biotrophic parasites causing serious damage and reduction in crop yields. Several economically important genera parasitize various crop plants. The root-knot, root lesion, and cyst nematodes are the three most economically damaging genera of PPNs on crops within the family Heteroderidae. It is very important to devise various management strategies against PPNs in economically important crop plants. Genetic engineering has proven a promising tool for the development of biotic and abiotic stress tolerance in crop plants. Additionally, the genetic engineering leading to transgenic plants harboring nematode resistance genes has demonstrated its significance in the field of plant nematology. Here, we have discussed the use of genetic engineering for the development of nematode resistance in plants. This review article also provides a detailed account of transgenic strategies for the resistance against PPNs. The strategies include natural resistance genes, cloning of proteinase inhibitor coding genes, anti-nematodal proteins and use of RNA interference to suppress nematode effectors. Furthermore, the manipulation of expression levels of genes induced and suppressed by nematodes has also been suggested as an innovative approach for inducing nematode resistance in plants. The information in this article will provide an array of possibilities to engineer resistance against PPNs in different crop plants.

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Section: Use Of Proteinase Inhibitor Coding Genesmentioning

confidence: 99%

Section: Use Of Proteinase Inhibitor Coding Genesmentioning

confidence: 99%

Section: Use Of Proteinase Inhibitor Coding Genesmentioning

confidence: 99%

Section: Use Of Proteinase Inhibitor Coding Genesmentioning

confidence: 99%

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Transgenic Strategies for Enhancement of Nematode Resistance in Plants

et al. 2017

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“…Most importantly, herbivorous insects have evolved a range of strategies to cope with dietary protease inhibitors, typically involving the secretion of complex midgut protease complements, the overexpression of inhibitor-sensitive proteases to outnumber the ingested inhibitors and the up-regulation of protease isoforms weakly sensitive to inhibition8. Nevertheless, and despite numerous unsuccessful attempts to use protease inhibitors in pest control, a number of promising cases have been reported recently1314151617181920 that still remind the importance of digestive proteases in herbivorous pests and the possible relevance of these enzymes as effective targets for crop improvement21222324.…”

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confidence: 99%

Functional proteomics-aided selection of protease inhibitors for herbivore insect control

Rasoolizadeh¹,

Munger²,

Goulet³

et al. 2016

Sci Rep

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Studies have reported the potential of protease inhibitors to engineer insect resistance in transgenic plants but the general usefulness of this approach in crop protection still remains to be established. Insects have evolved strategies to cope with dietary protease inhibitors, such as the use of proteases recalcitrant to inhibition, that often make the selection of effective inhibitors very challenging. Here, we used a functional proteomics approach for the ‘capture’ of Cys protease targets in crude protein extracts as a tool to identify promising cystatins for plant improvement. Two cystatins found to differ in their efficiency to capture Cys proteases of the coleopteran pest Leptinotarsa decemlineata also differed in their usefulness to produce transgenic potato lines resistant to this insect. Plants expressing the most potent cystatin at high level had a strong repressing effect on larval growth and leaf intake, while plants expressing the weakest cystatin showed no effect on both two parameters compared to untransformed parental line used for genetic transformation. Our data underline the relevance of considering the whole range of possible protease targets when selecting an inhibitor for plant pest control. They also confirm the feasibility of developing cystatin-expressing transgenics resistant to a major pest of potato.

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