Pyridine nucleotides in the thyroid

Pyridine Nucleotides in the Thyroid Gland. VIII. Effect of Thyroid-Stimulating Hormone on Nicotinic Acid-14C Incorporation Into NAD, NADP and NADPH

“…At the termination of the incubation period, the slices were washed in cold saline, blotted on filter paper, placed in 2 ml of extraction medium in a boiling water bath for 90 sec and homogenized. The extraction media used were 0.25M glycylglycine buffer pH 2.0 for oxidized pyridine nucleotides and 0.25M glycylglycine buffer pH 12.0 for reduced pyridine nucleotides (6).…”

“…The tubes containing fractions A, B and C were centrifuged in the cold and aliquots were neutralized for assay of pyridine nucleotides as described previously (6). 1 mg of unlabeled NAD and NADP was added to the remainder of the supernatant fraction as carrier.…”