Pyrolysis-gas chromatographic estimation of tobacco alkaloids and neophytadiene

Rosa, Nestor

doi:10.1016/s0021-9673(01)95326-9

Cited by 9 publications

(5 citation statements)

References 4 publications

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“…The calibration curve for the standard nicotine solutions (2.5-10.0 mg/mL) was linear with a correlation coefficient (R 2 ) of 0.999, when the analysis was carried out under the optimum conditions, as described above. This coefficient value is very high as compared to that of 0.983 obtained by Rosa, using the fixed temperature Victoreen pyrolyzer (11). The main reasons for the better linearity and accuracy of our method are probably due to our use of an external standard and our use of a temperature program, rather than a fixed temperature for nicotine desorption.…”

Section: Resultsmentioning

confidence: 55%

“…There have been several analytical approaches to the analysis of nicotine and minor alkaloids in tobacco, including the following methods: steam distillation spectrophotometry (5), micellar electrokinetic capillary chromatography (6), solvent extraction-gas chromatography (GC) (7)(8)(9)(10), pyrolysis-GC (11), solid-phase microextraction (SPME)-GC (12,13), and high-performance capillary electrophoresis (14).…”

Section: Introductionmentioning

confidence: 99%

“…In 1979, Rosa (11) reported a pyrolysis-GC method for the estimation of nicotine and nornicotine in tobacco, using a Victoreen pyrolyzer. The pyrolyzer conditions used in this method were fixed temperature (250 °C).…”

Section: Introductionmentioning

confidence: 99%

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Fast Analysis of Nicotine in Tobacco Using Double-Shot Pyrolysis−Gas Chromatography−Mass Spectrometry

Lee

Kwag

et al. 2007

J. Agric. Food Chem.

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A simple, rapid, and sensitive assay of nicotine in tobacco has been developed using pyrolysis-gas chromatography-mass spectrometry (DSP-GC-MS). The optimum pyrolyzer (desorption) conditions, using Korean tobacco grade B1O (0.4 mg) and n-heptadecane as an internal standard, were found to be heating from 50 to 300 degrees C at a rate of 60 degrees C/min. Replicate determinations (n = 5), on the same tobacco and using n-heptadecane as the internal standard, resulted in a nicotine content of 1.96%, with a relative standard deviation (RSD) of 1.9%. This result was in good agreement with those from established methods: the Cai ether extraction method, a chloroform extraction method, and the CORESTA recommended method. However, the DSP method requires less than half the time of the solvent extraction methods, requires less sample, is almost solvent-free, and is less labor-intensive. The DSP method was used to determine the nicotine content of eight flue-cured tobaccos from Brazil, China, Korea, and the United States, which were found to have contents ranging from 1.21 to 2.19%.

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Section: Resultsmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

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Fast Analysis of Nicotine in Tobacco Using Double-Shot Pyrolysis−Gas Chromatography−Mass Spectrometry

Lee

Kwag

et al. 2007

J. Agric. Food Chem.

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“…Other methods available for the analysis of alkaloids include near-infrared reflectance spectroscopy (NIRS) (McClure and Williamson 1986), lipophilic gel chromatography (Snook and Chortyk 1986), radioimmunoassay (Castro et al 1979), mass spectrometry (Green et al 1980), and pyrolysis-gas chromatography (Rosa 1979). Only a few of these procedures have been described in detail.…”

Section: Alternative Methodsmentioning

confidence: 99%

Alkaloids

1994

Modern Methods of Plant Analysis

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“…Modern analytical techniques, such as gas chromatography and gas chromatography combined with mass spectrometry, have become important tools in investigating the chemical constituents of tobacco and tobacco smoke (12,27,31,35). H.u.Jarr and Scmn.TEN (17) wed field-ionization mass spectrometry to profile three different commercial tobacco blends, and were able to carry out a rapid screening of the tobacco samples.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Tobacco Fractionation Procedure Using Pyrolysis Mass Spectrometry Combined with Multivariate Analysis

Scheijen

Boer

Boon

et al. 1989

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

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SUMMARYThis paper presents an evaluation of a fractionation procedure for use with tobacco. Correlation coefficients calculated from the weights of the polymer fractions obtained and data obtained with classical wetchemical methods show that these parameters have a low resolving power, which precludes any detailed distinction between tobaccos and tobacco-derived fractions. Pyrolysis mass spectrometry combined with multivariate analysis is presented as a promising approach for investigating the variability in the chemical composition of tobacco. Multivariate analysis performed on the pyrolysis mass-spectrometric fingerprints of all tobacco fractions indicates that the differences between the individual tobaccos are smaller than the differences induced by the fractionation procedure. Multivariate analysis of subfdes of the pyrolysis mass-spectrometric fingerprints of separate polymer fractions is an excel- lent method for classifying tobaccos. Leaf and stem material can easily be distinguished. A further differentiation is made between the flue-cured and Burley-type tobaccos. The strong clustering of tobacco samples after treatment with potassium hydroxide points to a uniform cell-wall skdeton. The greater variability in the ethanolized tobacco samples, and samples subjected to hot-water extractia:n or amylase digestion, is related to the cytosol characteristics. These fractions appear to reflect both the dissimilarities between the distinct tobacco types and phenotypic variations due to differences in cultural management. Pyrolysis mass spectrometry of the K.lason lignin residues points to a large variety of chemical constituents unrelated to lignin. Pyrolysis gas-chromatography mass-spectrometric data on the K.lason lignin residues of a Burley tobacco showed that the correlation made between the organic-nitrogen content and the protein content of this fraction might be misleading. As a consequence the maximum value for the lignin content of this tobacco is estimated incorrectly. sieurs variables (multivariance) se rC:vele une approche prometteuse pour !'etude des differences de composition chimique des tabacs. L'analyse des spectres de masse (fingerprints) obtenus aprh pyrolyse de toutes les fractions de tabac montre que les differences existant entre les divers types de tabac soot plus faibles que celles qui soot induites par la mC:tbode de fractionnement. L'analyse (multivariance) realisee separement sur les spectres de masse des differentes fractions polymeres est une excellente methode pour la classification des tabacs. Les cOtes et les feuilles se distinguent facilement; le tabac Hurley et le tabac «flue-cured• soot aussi nettement differencies. La formation marquee de grappes (cluster) parmi les ecbantillons apres traitement par l'hydroxyde de potassium indique que la structure de la paroi cellulaire semble etre la meme chez tous les tabacs. Apres extraction par l'Ctbanol suivie d'une extraction par I' eau chaude ou d'un traitement par !'amylase, on obtient dans chaque cas des residus presentant une varia...

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Pyrolysis-gas chromatographic estimation of tobacco alkaloids and neophytadiene

Cited by 9 publications

References 4 publications

Fast Analysis of Nicotine in Tobacco Using Double-Shot Pyrolysis−Gas Chromatography−Mass Spectrometry

Fast Analysis of Nicotine in Tobacco Using Double-Shot Pyrolysis−Gas Chromatography−Mass Spectrometry

Alkaloids

Evaluation of a Tobacco Fractionation Procedure Using Pyrolysis Mass Spectrometry Combined with Multivariate Analysis

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