Thermo distillation of palm kernel shell in a column reactor was studied in this paper. The objective of this research was to characterize the bio oil and bio oil fractions. The maximum yield was around 70 wt% at 120 °C. The bio oil fractions were collected in ten columns at different temperature ranging between 75- 105°C. HHV of bio oil was 26MJ/Kg. The bio oil moisture, volatility, fixed carbon, and ash were determined and found to be around 6.44wt%, 52.72wt%, 24.39wt%, 16.45wt%, respectively. It can be seen that the PKS bio oil can be considered as an alternative fuel. . HHV of bio oil fraction was between 20- 21MJ/Kg, The density of bio oil fraction was 976.54 g/ mL, and pH of bio oil fraction were around of 2.16.