Pyruvate diminishes the cytotoxic activity of ascorbic acid in several tumor cell lines in vitro

Rodemeister, Sandra; Hill, Katharina

doi:10.1016/j.bbrc.2017.09.138

Cited by 7 publications

(1 citation statement)

References 21 publications

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“…Some studies have reported that polyphenolic and others antioxidant compounds might react with culture medium components generating hydrogen peroxide in situ. 36 , 37 This situation is dependent on diverse experimental conditions such as the selected culture medium and sample concentration range. 38 , 39 Probably, under our experimental conditions, MC3T3-E1 cells were slightly more sensitive to AC-induced H 2 O 2 production than fibroblasts (FG), as described elsewhere.…”

Section: Discussionmentioning

confidence: 99%

Standardized Arrabidaea chica Extract Shows Cytoprotective Effects in Zoledronic Acid-Treated Fibroblasts and Osteoblasts

Zago¹,

Sousa²,

Servat-Medina³

et al. 2020

CCIDE

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Introduction: Osteonecrosis of the jaw is a condition associated with intraoral ulceration and bone necrosis induced by antiresorptive medications, such as zoledronic acid, a bisphosphonate. Previous data on Arrabidaea chica (H&B.) Verlot wound healing activity prompted the study reported herein on A. chica standardized hydro alcoholic extract in vitro cytoprotective activity data on epithelial and osteoblastic cells exposed to zoledronic acid (ZA). Methods: Primary human gingival fibroblasts and murine pre-osteoblasts were treated with ZA 10 µM together with 5 or 10 µg.mL −1 A. chica extract for 24h and 48 h. At both times, cells were submitted to viability assay and caspase 3/7 activation evaluation. Statistical analysis used one-way ANOVA and p=0.05. Results: In cell viability assay, a drastic damage effect of ZA appeared after 48 h in both epithelial (55.8%) and pre-osteoblastic cells (39.7%). When treated with ZA in combination with A. chica extract, cells showed higher viability values: 74.1%-82.3% for fibroblasts and 66% for pre-osteoblasts. Furthermore, the combined treatment presented lower caspase 3/7 activation in fibroblasts and pre-osteoblasts. Conclusion: At low concentrations, A. chica extract showed promising cytoprotective effects against ZA-induced damage actions; however, further in vitro and in vivo studies are required to establish the mechanism of action.

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