Pyruvate Kinase Isozymes among Human Organs and Blood Cells

Etiemble, Jeanne; Boivin, Pierre

doi:10.1159/000458872

Cited by 7 publications

(3 citation statements)

References 10 publications

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“…fetal constellation of pyruvate kinase isoenzyme such as in 3 and 5 months old fetus is observed (only isoenzyme K is present). In gonads of healthy males constellation K and M isoenzymes exist (similar constellation in healthy subject was observed by Etiemble -1976).…”

Section: -F Zymogram Of Supernatant From Human Spermatozoasupporting

confidence: 66%

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The Pyruvate Kinase Isoenzyme Pattern in Gonad Tissue from Individuals with Primary “Idiopathic” Hypogonadism

et al. 2009

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Summary Isoenzyme pattern of pyruvate kinase was determined in human gonads in the ontogenic evolution and in gonads of patients with primary, “idiopathic” hypogonadism. In the gonads of adult men with normal testicular function the isoenzyme K and M of pyruvate kinase was found. The possibility that isoenzyme M originated from spermatozoa was eliminated by performing electrophoresis of pyruvate kinase in spermatozoa homogenates. In the testes from human fetus isoenzyme K of pyruvate kinase was detected. The same pattern was observed in goads of patients with primary “idiopathic” hypogonadism (only isoenzyme K of pyruvate kinase). These results show the fetal pattern of isoenzyme K of pyruvate kinase (regulating enzyme) in the testes of adult men with primary hypogonadism. This relation is not reflected in histological studies. These findings suggest abnormal development of the gonads in fetal period. This is an indirect, biochemical proof which however cannot be rejected when considering etiopathogenesis of above pathology. Das Pyruvat‐Kinase‐Isoenzym‐Muster im Keimdrüsengewebe von Individuen mit primärem, idiopathischen Hypogonadismus Zusammenfassung In menschlichen Gonaden verschiedener Embryonalstadien und in solchen von Patienten mit einem primären, idiopathischen Hypogonadismus wurden die Isoenzymmuster der Pyruvatkinase bestimmt. In den Keimdrüsen von erwachsenen Männern mit einer normalen Hodenfunktion konnten die Isoenzyme K und M der Pyruvatkinase gefunden werden. Durch eine Elektrophorese der Pyruvatkinase in Spermatozoen‐Homogenaten konnte ausgeschlossen werden, daß das Isoenzym M aus den Spermatozoen stammt, was als möglich diskutiert wurde. In den Hoden von menschlichen Foeten ließ sich das Isoenzym K der Pyruvatkinase bestimmen. Das gleiche Muster wurde in den Keimdrüsen von Patienten mit einem primären, idiopathischen Hypogonadismus (ausschließlich Isoenzym K der Pyruvatkinase) nachgewiesen. Diese Ergebnisse zeigen das foetale Muster des Isoenzym K der Pyruvatkinase (Regulationsenzym) in den Hoden von erwachsenen Männern mit einem primären Hypogonadismus. Dieser Zusammenhang spiegelt sich nicht in den histologischen Studien wieder. Die Ergebnisse lassen an eine abnormale Entwicklung der Gonaden in der Foetalperiode denken. Das ist ein indirekter, biochemischer Beweis, der unter Berücksichtigung der Aetiopathogenese nicht verworfen werden kann.

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Section: -F Zymogram Of Supernatant From Human Spermatozoasupporting

confidence: 66%

“…In gonads of healthy males constellation K and M isoenzymes exist (similar constellation in healthy subject was observed by Etiemble -1976). Therefore it is advisable to use other methods eg.…”

Section: -F Zymogram Of Supernatant From Human Spermatozoamentioning

confidence: 68%

The Pyruvate Kinase Isoenzyme Pattern in Gonad Tissue from Individuals with Primary “Idiopathic” Hypogonadism

et al. 2009

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“…The timing and location of extracellular PKM2 appearance matched well with that of neutrophil infiltration at wound site (Figure A–C), suggesting that PKM2 might be released from neutrophils during wound repair. Consistently, it is known that activated neutrophils express PKM2 . Macrophages arrive the wound site following neutrophil infiltration.…”

Section: Resultsmentioning

confidence: 59%

PKM2 released by neutrophils at wound site facilitates early wound healing by promoting angiogenesis

Zhang

Liu

et al. 2016

Wound Repair Regeneration

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Neutrophils infiltration/activation following wound induction marks the early inflammatory response in wound repair. However, the role of the infiltrated/activated neutrophils in tissue regeneration/proliferation during wound repair is not well understood. Here, we report that infiltrated/activated neutrophils at wound site release pyruvate kinase M2 (PKM2) by its secretive mechanisms during early stages of wound repair. The released extracellular PKM2 facilitates early wound healing by promoting angiogenesis at wound site. Our studies reveal a new and important molecular linker between the early inflammatory response and proliferation phase in tissue repair process.

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Complex kinetics of human leukocyte and platelet pyruvate kinases

Etiemble

Boivin

1977

Biochimica et Biophysica Acta (BBA) - Enzymology

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Pyruvate Kinase Isozymes among Human Organs and Blood Cells

Cited by 7 publications

References 10 publications

The Pyruvate Kinase Isoenzyme Pattern in Gonad Tissue from Individuals with Primary “Idiopathic” Hypogonadism

The Pyruvate Kinase Isoenzyme Pattern in Gonad Tissue from Individuals with Primary “Idiopathic” Hypogonadism

PKM2 released by neutrophils at wound site facilitates early wound healing by promoting angiogenesis

Complex kinetics of human leukocyte and platelet pyruvate kinases

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