Pyruvate overflow and carbon flux within the central metabolic pathways of Corynebacterium glutamicum during growth on lactate

Cocaign‐Bousquet, Muriel; Lindley, Nic D.

doi:10.1016/0141-0229(94)00023-k

Cited by 66 publications

(60 citation statements)

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“…7,13,24) Our study also demonstrates that C. glutamicum 2262 can utilize lactate formed during the aerobic phase when glucose is exhausted. This is in agreement with previously reported studies, indicating that C. glutamicum is able to grow using lactate as sole carbon source under aerobic conditions, 25,26) probably through respiratory quinone-dependent LDH. 27,28) In contrast, under oxygen deprivation, lactate was consumed only when glucose was exhausted in the culture medium.…”

Section: Discussionsupporting

confidence: 83%

Lactate production as representative of the fermentation potential of Corynebacterium glutamicum 2262 in a one-step process

Khuat

Kaboré

Olmos

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionsupporting

confidence: 83%

Lactate production as representative of the fermentation potential of Corynebacterium glutamicum 2262 in a one-step process

Khuat

Kaboré

Olmos

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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“…Besides PEPCx, PEPCk, PCx and oxaloacetate decarboxylase, the decarboxylating/carboxylating enzymes located at the metabolic node around pyruvate in C. glutamicum include the NADP-dependent malic enzyme (Cocaign-Bousquet & Lindley, 1995 ;Vallino & Stephanopoulos, 1993). In principle, the reaction catalysed by this enzyme is reversible, but in most organisms the malic enzyme is assumed to catalyse the oxidative decarboxylation of malate rather than the reductive carboxylation of pyruvate (Kornberg, 1966).…”

Section: Discussionmentioning

confidence: 99%

Pyruvate carboxylase as an anaplerotic enzyme in Corynebacterium glutamicum

et al. 1997

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The recent discovery that phosphoenolpyruvate carboxylase (PEPCx) is dispensable for growth and lysine production in Corynebacterium glutamicum implies that this organism possesses (an) alternative anaplerotic enzyme(s). In permeabilized cells of C. glutamicum, w e detected pyruvate carboxylase (PCx) activity. This activity was effectively inhibited by low concentrations of ADP, AMP and acetyl-CoA. PCx activity was highest [45 2 5 nmol min'l (mg dry wt) ''] in cells grown on lactate or pyruvate, and was about two-to threefold lower when the cells were grown on glucose or acetate, suggesting that formation of PCx is regulated by the carbon source in the growth medium. In cells grown a t low concentrations of biotin (< 5 pg I-'), PCx activity was drastically reduced, indicating that the enzyme is a biotin protein. Growth experiments with the wild-type and a defined PEPCx-negative mutant of C. glutamicum on glucose showed that the mutant has a significantly higher demand for biotin than the wild-type, whereas both strains have the same high biotin requirement for growth on lactate and the same low biotin requirement for growth on acetate. These results indicate that (i) PCx is an essential anaplerotic enzyme for growth on glucose in the absence of PEPCx, (ii) PCx is an essential anaplerotic enzyme for growth on lactate even in the presence of PEPCx, and (iii) PCx has no anaplerotic significance for growth on acetate as the carbon source. In support of these conclusions, screening for clones unable to grow on a minimal medium containing lactate, but able to grow on a medium containing glucose or acetate, led to the isolation of PCx-defective mutants of C. glutamicum .~~~ ~~

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“…For glucose, an alternative uptake system involving intracellular phosphorylation of glucose by a glucokinase has been identified [10]. In addition, C. glutamicum can grow on different organic acids such as gluconate [11], lactate, [12], acetate [13], propionate [14], or citrate [15]. For growth and also for amino acid overproduction, cells further require nitrogen and sulfur as major elements in addition to carbon.…”

Section: Nutritional Requirements and Assimilatory Pathwaysmentioning

confidence: 99%

“…A number of studies utilized stoichiometric balancing to assess the flexibility of the metabolic network [11,75] and to investigate the influence of environmental conditions such as dissolved oxygen level [88], salt content [89], or nutrient status [12,90,91]. However, this conventional approach cannot yield reliable information about parallel or bidirectional reactions and has to rely on balances for NADH or NADPH, which may not be accurate [34].…”

Section: Conventional Metabolic Flux Analysis By Stoichiometric Balanmentioning

confidence: 99%

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Analysis and Engineering of Metabolic Pathway Fluxes in Corynebacterium glutamicum

Wittmann

2010

Biosystems Engineering I

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The Gram-positive soil bacterium Corynebacterium glutamicum was discovered as a natural overproducer of glutamate about 50 years ago. Linked to the steadily increasing economical importance of this microorganism for production of glutamate and other amino acids, the quest for efficient production strains has been an intense area of research during the past few decades. Efficient production strains were created by applying classical mutagenesis and selection and especially metabolic engineering strategies with the advent of recombinant DNA technology. Hereby experimental and computational approaches have provided fascinating insights into the metabolism of this microorganism and directed strain engineering. Today, C. glutamicum is applied to the industrial production of more than 2 million tons of amino acids per year. The huge achievements in recent years, including the sequencing of the complete genome and efficient post genomic approaches, now provide the basis for a new, fascinating era of research -analysis of metabolic and regulatory properties of C. glutamicum on a global scale towards novel and superior bioprocesses.

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Pyruvate overflow and carbon flux within the central metabolic pathways of Corynebacterium glutamicum during growth on lactate

Cited by 66 publications

References 19 publications

Lactate production as representative of the fermentation potential of Corynebacterium glutamicum 2262 in a one-step process

Lactate production as representative of the fermentation potential of Corynebacterium glutamicum 2262 in a one-step process

Pyruvate carboxylase as an anaplerotic enzyme in Corynebacterium glutamicum

Analysis and Engineering of Metabolic Pathway Fluxes in Corynebacterium glutamicum

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