Pyruvate-triggered TCA cycle regulation in Staphylococcus aureus promotes tolerance to betamethasone valerate

Matsumoto, Yasuhiko; Nakashima, Takumi; Cho, Otomi; Ohkubo, Tomotaka; Kato, Jumpei; Sugita, Takashi

doi:10.1016/j.bbrc.2020.05.035

Cited by 12 publications

(13 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Their high affinities for fumarate and malate indicate that they are specifically adapted to play reciprocal roles in the overall fumarate oxidation process via the citric acid cycle (FUMA) and in the ultimate reduction of malate by providing fumarate, which serves as an anaerobic electron acceptor (FUMB) ( Woods et al, 1988 ). In this study, the effects of the proteins, PflD, DcuB, DcuC, and FumA, on the sensitivity of E. coli to plantaricin BM-1 were similar to those reported by Matsumoto et al (2020) . They found that Staphylococcus aureus regulates the TCA cycle by producing pyruvate and reducing its tolerance to betamethasone valerate (BV).…”

Section: Discussionsupporting

confidence: 89%

BasS/BasR Two-Component System Affects the Sensitivity of Escherichia coli to Plantaricin BM-1 by Regulating the Tricarboxylic Acid Cycle

et al. 2022

View full text Add to dashboard Cite

Plantaricin BM-1, a class IIa bacteriocin produced by Lactiplantibacillus plantarum BM-1, exhibits significant antibacterial activity against many gram-positive and gram-negative bacteria. However, the mechanism underlying the action of class IIa bacteriocins against gram-negative bacteria remains to be explored. This study aimed to investigate the role of the BasS/BasR two-component system (TCS) in Escherichia coli (E. coli) K12 response to plantaricin BM-1. The IC50 values for plantaricin BM-1 in E. coli K12, basS mutant (E. coli JW4073), and basR mutant (E. coli JW4074) strains were found to be 10.85, 8.94, and 7.62 mg/mL, respectively. Growth curve experiments showed that mutations in the BasS/BasR TCS led to an increase in the sensitivity of E. coli K12 to plantaricin BM-1 and that after gene complementation, the complemented mutant strain regained its original sensitivity. Proteomic analysis showed that 100 and 26 proteins were upregulated and 62 and 58 proteins were downregulated in E. coli JW4073 and E. coli JW4074, respectively. These differential proteins, which exhibited different molecular functions and participated in different molecular pathways, were mainly concentrated in the cytoplasm. More specifically, mutations in basS and basR were found to affect the synthesis and metabolism of many substances in E. coli, including many important amino acids and enzymes involved in cellular activities. In addition, 14 proteins, including 8 proteins involved in the tricarboxylic acid (TCA) cycle, were found to be downregulated in both E. coli JW4073 and E. coli JW4074. Growth curve experiments showed that the deletion of these proteins could increase the sensitivity of E. coli to plantaricin BM-1. Therefore, we speculate that TCA pathway regulation may be an important mechanism by which the BasS/BasR TCS regulates the sensitivity of E. coli to plantaricin BM-1. This finding will facilitate the determination of the mechanism underlying the action of class IIa bacteriocins against gram-negative bacteria.

show abstract

Section: Discussionsupporting

confidence: 89%

BasS/BasR Two-Component System Affects the Sensitivity of Escherichia coli to Plantaricin BM-1 by Regulating the Tricarboxylic Acid Cycle

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Bacterial growth inhibition assays were performed according to a previous report [ 24 ]. Samples including D .…”

Section: Methodsmentioning

confidence: 99%

“…Bacterial growth inhibition assays were performed according to a previous report [24]. Samples including D. acidovorans CS and inhibitors were diluted with NB to the appropriate concentrations and dispensed in 50-μL aliquots into a 96-well plate (TPP).…”

Section: Bacterial Growth Inhibition Assaymentioning

confidence: 99%

See 1 more Smart Citation

Delftia acidovorans secretes substances that inhibit the growth of Staphylococcus epidermidis through TCA cycle-triggered ROS production

et al. 2021

Self Cite

View full text Add to dashboard Cite

The proportion of Staphylococcus aureus in the skin microbiome is associated with the severity of inflammation in the skin disease atopic dermatitis. Staphylococcus epidermidis, a commensal skin bacterium, inhibits the growth of S. aureus in the skin. Therefore, the balance between S. epidermidis and S. aureus in the skin microbiome is important for maintaining healthy skin. In the present study, we demonstrated that the heat-treated culture supernatant of Delftia acidovorans, a member of the skin microbiome, inhibits the growth of S. epidermidis, but not that of S. aureus. Comprehensive gene expression analysis by RNA sequencing revealed that culture supernatant of D. acidovorans increased the expression of genes related to glycolysis and the tricarboxylic acid cycle (TCA) cycle in S. epidermidis. Malonate, an inhibitor of succinate dehydrogenase in the TCA cycle, suppressed the inhibitory effect of the heat-treated culture supernatant of D. acidovorans on the growth of S. epidermidis. Reactive oxygen species production in S. epidermidis was induced by the heat-treated culture supernatant of D. acidovorans and suppressed by malonate. Further, the inhibitory effect of the heat-treated culture supernatant of D. acidovorans on the growth of S. epidermidis was suppressed by N-acetyl-L-cysteine, a free radical scavenger. These findings suggest that heat-resistant substances secreted by D. acidovorans inhibit the growth of S. epidermidis by inducing the production of reactive oxygen species via the TCA cycle.

show abstract

“…It is one of the greatest pathogens for nosocomial and community infections [1][2][3][4]. This germ is closely related to the occurrence of pyogenic and toxic diseases in human beings, such as pneumonia, endocarditis, and septicemia [5][6][7]. Moreover, it is noteworthy to mention that S. aureus is an important foodborne pathogen, second only to Escherichia coli [8,9].…”

Section: Introductionmentioning

confidence: 99%

Rapid Analysis for Staphylococcus aureus via Microchip Capillary Electrophoresis

Chen

Sun

Peng

et al. 2021

Sensors

View full text Add to dashboard Cite

Staphylococcus aureus (S. aureus) is one of the most common pathogens for nosocomial and community infections, which is closely related to the occurrence of pyogenic and toxic diseases in human beings. In the current study, a lab-built microchip capillary electrophoresis (microchip CE) system was employed for the rapid determination of S. aureus, while a simple-to-use space domain internal standard (SDIS) method was carried out for the reliable quantitative analysis. The precision, accuracy, and reliability of SDIS were investigated in detail. Noted that these properties could be elevated in SDIS compared with traditional IS method. Remarkably, the PCR products of S. aureusnuc gene could be identified and quantitated within 80 s. The theoretical detection limit could achieve a value of 0.066 ng/μL, determined by the using SDIS method. The current work may provide a promising detection strategy for the high-speed and highly efficient analysis of pathogens in the fields of food safety and clinical diagnosis.

show abstract

Pyruvate-triggered TCA cycle regulation in Staphylococcus aureus promotes tolerance to betamethasone valerate

Cited by 12 publications

References 16 publications

BasS/BasR Two-Component System Affects the Sensitivity of Escherichia coli to Plantaricin BM-1 by Regulating the Tricarboxylic Acid Cycle

BasS/BasR Two-Component System Affects the Sensitivity of Escherichia coli to Plantaricin BM-1 by Regulating the Tricarboxylic Acid Cycle

Delftia acidovorans secretes substances that inhibit the growth of Staphylococcus epidermidis through TCA cycle-triggered ROS production

Rapid Analysis for Staphylococcus aureus via Microchip Capillary Electrophoresis

Contact Info

Product

Resources

About