Qingkailing Injection Alleviates Experimental Autoimmune Uveitis in Rats <i>via</i> Inhibiting Th1 and Th17 Effector Cells

Abstract

Qingkailing injection is a well-known composite formula of traditional Chinese medicine and is commonly used in clinical practice, which could offer immunomodulatory effect from our clinical experience on uveitis treatment by Qingkailing. We did the experiment in order to investigate the curative effect and mechanism of Qingkailing injection to rat experimental autoimmune uveitis (EAU). EAU was induced in Lewis rats by immunization IRBP1177-1191 in complete Freund's adjuvant in multi-point. We found that Qingkailing injection can alleviate autoimmune uveitis in rats, inhibit the differentiation toward Th1 and Th17 effector cells and the relevant cytokines secretion. The therapeutic effect may also be regulated through increased secretion of interleukin (IL)-10.

Characterization of microRNA expression profiling in peripheral blood lymphocytes in rats with experimental autoimmune uveitis

“…A combinatorial strategy was used where target miRNAs were predicted for the differentially expressed genes using microcosm, miRanda and miRDB Characterization of microRNA expression profiling in peripheral… enrichment and KEGG pathway analysis. Importantly, our previous study has substantiated elevated levels of IL-10 and IL-17 in EAU rats compared with normal ones [21].…”

“…For uveitis, it is involved in increased levels of inflammatory cytokines and decreased levels of regulatory cytokines, including interleukin (IL)-17 [16,17], IL-21 [18,19], IL-10 [20,21], IFN-c [22,23] and so on. Meanwhile, it has also been reported that toll-like receptors (TLRs) are closely associated with the pathogenesis of uveitis, such as TLR2 [24,25], TLR4 [24][25][26][27][28] and other TLRs [25].…”

Herbal Medicines for the Treatment of Functional and Inflammatory Bowel Disorders

“…Many herbal preparations have immunomodulatory effects. [47][48][49][50][51][52][53][54][55][56][57] Thus it appears reasonable to trial herbal medications in patients with IBD. On the other hand, good, large clinical trials testing the effects of herbal therapy in IBD are widely lacking.…”

UHPLC-MS for the analytical characterization of traditional Chinese medicines

“…Qingkailing (QKL) injection is widely used in the clinic for antipyretic and anti-inflammatory effects [112], but its serious adverse drug reactions have attracted attention. Caffeoylquinic acids in QKL are responsible for these adverse drug reactions.…”