Qishen Granule alleviates endoplasmic reticulum stress-induced myocardial apoptosis through IRE-1-CRYAB pathway in myocardial ischemia

Zhang, Qian; Shi, Jun; Guo, Dongqing; Wang, Qiyan; Yang, Xiaomin; Lü, Wei; Sun, Xiaoqian; He, Hao; Li, Ning; Wang, Yong; Li, Chun; Wang, Wei

doi:10.1016/j.jep.2020.112573

Cited by 27 publications

(10 citation statements)

References 32 publications

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“…To investigate the underlying mechanisms of METRNL in MI/R injury, further experiments were performed to confirm whether METRNL had a regulative effect on ER stress in H9C2 cells. Accumulating evidence has demonstrated that ER stress plays a crucial role in myocardial ischemia [ 24 ]. Excessive inflammatory response and oxidative stress impair the ER functions and cause accumulation of unfolded/misfolded proteins, triggering activation of ER stress [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Meteorin-Like (METRNL) Attenuates Myocardial Ischemia/Reperfusion Injury-Induced Cardiomyocytes Apoptosis by Alleviating Endoplasmic Reticulum Stress via Activation of AMPK-PAK2 Signaling in H9C2 Cells

Cai

Wang

et al. 2020

Med Sci Monit

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Section: Discussionmentioning

confidence: 99%

Meteorin-Like (METRNL) Attenuates Myocardial Ischemia/Reperfusion Injury-Induced Cardiomyocytes Apoptosis by Alleviating Endoplasmic Reticulum Stress via Activation of AMPK-PAK2 Signaling in H9C2 Cells

Cai

Wang

et al. 2020

Med Sci Monit

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“…The fingerprint spectrum was further established by the high-performance liquid chromatography (HPLC) method in our previous studies [ 20 , 22 ]. The major components are formononetin, tanshinone IIA, tanshinone I, cryptotanshinone, and harpagoside [ 23 ]. The Chinese herbs were identified by Professor Jian Ni, School of Chinese Materia Medica, Beijing University of Chinese Medicine.…”

Section: Methodsmentioning

confidence: 99%

The Protective Effect of Qishen Granule on Heart Failure after Myocardial Infarction through Regulation of Calcium Homeostasis

Yang

Wang

Zeng

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Qishen granule (QSG) is a frequently prescribed traditional Chinese medicine formula, which improves heart function in patients with heart failure (HF). However, the cardioprotective mechanisms of QSG have not been fully understood. The current study aimed to elucidate whether the effect of QSG is mediated by ameliorating cytoplasmic calcium (Ca2+) overload in cardiomyocytes. The HF rat model was induced by left anterior descending (LAD) artery ligation surgery. Rats were randomly divided into sham, model, QSG-low dosage (QSG-L) treatment, QSG-high dosage (QSG-H) treatment, and positive drug (diltiazem) treatment groups. 28 days after surgery, cardiac functions were assessed by echocardiography. Levels of norepinephrine (NE) and angiotensin II (AngII) in the plasma were evaluated. Expressions of critical proteins in the calcium signaling pathway, including cell membrane calcium channel CaV1.2, sarcoendoplasmic reticulum ATPase 2a (SERCA2a), calcium/calmodulin-dependent protein kinase type II (CaMKII), and protein phosphatase calcineurin (CaN), were measured by Western blotting (WB) and immunohistochemistry (IHC). Echocardiography showed that left ventricular ejection fraction (EF) and fractional shortening (FS) value significantly decreased in the model group compared to the sham group, and illustrating heart function was severely impaired. Furthermore, levels of NE and AngII in the plasma were dramatically increased. Expressions of CaV1.2, CaMKII, and CaN in the cardiomyocytes were upregulated, and expressions of SERCA2a were downregulated in the model group. After treatment with QSG, both EF and FS values were increased. QSG significantly reduced levels of NE and AngII in the plasma. In particular, QSG prevented cytoplasmic Ca2+ overload by downregulating expression of CaV1.2 and upregulating expression of SERCA2a. Meanwhile, expressions of CaMKII and CaN were inhibited by QSG treatment. In conclusion, QSG could effectively promote heart function in HF rats by restoring cardiac Ca2+ homeostasis. These findings revealed novel therapeutic mechanisms of QSG and provided potential targets in the treatment of HF.

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“…According to the report, salidroside can be used as an effective anti-apoptotic neuroprotective agent protecting ischemic stroke by regulating the PI3K/Akt mediated apoptotic pathway (Zhang et al, 2018c) and the complement system (Wang et al, 2020c). Zhang et al found that asiaticoside can reverse the apoptotic process through blocking the NOD2/ MAPK/NF-κB signal pathway, which could reduce nerve damage, brain edema, infarct size, inflammation, and oxidative stress in the CIRI model (Zhang et al, 2020b). Some studies announced that the active components of Scrophularia ningpoensis Hemsl.…”

Section: Active Ingredients Of Traditional Chinese Medicinementioning

confidence: 99%

Therapeutic targets by traditional Chinese medicine for ischemia-reperfusion injury induced apoptosis on cardiovascular and cerebrovascular diseases

Cheng

Hu²,

Liu³

et al. 2022

Front. Pharmacol.

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Traditional Chinese medicine (TCM) has a significant role in treating and preventing human diseases. Ischemic heart and cerebrovascular injuries are two types of diseases with different clinical manifestations with high prevalence and incidence. In recent years, it has been reported that many TCM has beneficial effects on ischemic diseases through the inhibition of apoptosis, which is the key target to treat myocardial and cerebral ischemia. This review provides a comprehensive summary of the mechanisms of various TCMs in treating ischemic cardiovascular and cerebrovascular diseases through anti-apoptotic targets and pathways. However, clinical investigations into elucidating the pharmacodynamic ingredients of TCM are still lacking, which should be further demystified in the future. Overall, the inhibition of apoptosis by TCM may be an effective strategy for treating ischemic cardio-cerebrovascular diseases.

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Qishen Granule alleviates endoplasmic reticulum stress-induced myocardial apoptosis through IRE-1-CRYAB pathway in myocardial ischemia

Cited by 27 publications

References 32 publications

Meteorin-Like (METRNL) Attenuates Myocardial Ischemia/Reperfusion Injury-Induced Cardiomyocytes Apoptosis by Alleviating Endoplasmic Reticulum Stress via Activation of AMPK-PAK2 Signaling in H9C2 Cells

Meteorin-Like (METRNL) Attenuates Myocardial Ischemia/Reperfusion Injury-Induced Cardiomyocytes Apoptosis by Alleviating Endoplasmic Reticulum Stress via Activation of AMPK-PAK2 Signaling in H9C2 Cells

The Protective Effect of Qishen Granule on Heart Failure after Myocardial Infarction through Regulation of Calcium Homeostasis

Therapeutic targets by traditional Chinese medicine for ischemia-reperfusion injury induced apoptosis on cardiovascular and cerebrovascular diseases

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