QTL analysis of resistance to Fusarium head blight in wheat using a ‘Frontana’‐derived population

Abstract

Fusarium head blight (FHB or head scab) has become a major limiting factor for sustainable wheat (Triticum aestivum L.) production around the world. For quantitative trait loci (QTL) analysis of resistance to FHB, F 3 plants and F 3 : 5 lines, derived from a ÔFrontanaÕ (moderately resistant)/ÔSeri82Õ (susceptible) cross, were spray-inoculated in 2001 and 2002, respectively. Artificial inoculations were carried out under field conditions. Of 273 SSR and AFLP markers, 250 could be mapped and they yielded 42 linkage groups, covering a genetic distance of 1931 cM. QTL analysis was based on the constructed linkage map and area under the disease progress curve (AUDPC). The analyses revealed three consistent QTLs associated with FHB resistance on chromosomes 1BL, 3AL and 7AS explaining 7.9%, 7.7% and 7.6% of the phenotypic variation, respectively, above 2 years. The results confirmed the previously described resistance QTL of ÔFrontanaÕ on chromosome 3AL. A combination of ÔFrontanaÕ resistance with ÔSumai-3Õ resistance may lead to lines with augmented resistance expression.

Mapping of QTL associated with Fusarium head blight in spring wheat RL4137

“…This highlights the importance of appropriate testing for the detection of some FHB QTL that are, perhaps, only effective under particular conditions. We observed FHB QTL on 2B and 7A, in common with the reports of Steiner et al (2004) and Mardi et al (2006), respectively. Both previous studies found Frontana to carry alleles on 1B that were deleterious for FHB resistance compared to the other parent.…”

“…In addition, a QTL of small effect on 1BL was contributed by Remus, the susceptible parent. Subsequently, Mardi et al (2006) confirmed the QTL on 3A and detected an additional QTL on 7AS. They also detected a QTL on 1BL originating from Seri82, the susceptible parent of their mapping population.…”

“…The QTL on 2B co-localized with that for the major FHB QTL in this population. Despite Frontana being acknowledged as a useful source of FHB resistance (Van Ginkel et al 1996), only two QTL studies have been reported to date (Steiner et al 2004;Mardi et al 2006). Steiner et al (2004) reported stable QTL for field resistance on 3A and 5A and less stable QTL on 2B and 6B in a Frontana/Remus cross.…”

“…In addition to this, in vitro experiments suggest that Frontana may be able to degrade and tolerate higher levels of DON (Miller & Arnison 1986;Wang & Miller 1988). Previous mapping studies indicate that resistance in Frontana is conditioned by several QTL of minor effect (Steiner et al 2004;Mardi et al 2006). RL4137 is a Canadian spring wheat derived from Frontana and was found to be highly resistant to FHB in preliminary trials at the John Innes Centre.…”

Assessing wheat (Triticum aestivum L.) genetic diversity using quality traits, amplified fragment length polymorphisms, simple sequence repeats and proteome analysis

“…SSR markers occur frequently in most eukaryote genomes and can be highly informative and reproducible (Gupta et al, 1996). The AFLP technique is laborious and time consuming but is also able to detect many polymorphisms with each primer combination (Mardi et al, 2005(Mardi et al, , 2006. Proteomic analysis showed to be a powerful approach to analyse crop proteomics (Salekdeh & Komatsu, 2007).…”