Excited states in the proton-unbound odd-odd nucleus 144 Ho have been populated using the 92 Mo( 54 Fe,pn) 144 Ho reaction and studied using the recoil-isomer-tagging technique. The alignment properties and signature splitting of the rotational band above the I π = (8 + ) 144m Ho isomer have been analyzed and the isomer confirmed to have a πh 11/2 ⊗ νh 11/2 two-quasiparticle configuration. The configuration-constrained blocking method has been used to calculate the shapes of the ground and isomeric states, which are both predicted to have triaxial nuclear shapes with |γ | ≈ 24 • .