Qualitative analysis of chemical constituents in traditional Chinese medicine analogous formula cheng‐Qi decoctions by liquid chromatography–mass spectrometry

Wen, Xuexun; Luo, Kedi; Xiao, Shun; Ni, Ai; Wang, Shufang; Fan, Xiaohui

doi:10.1002/bmc.3549

Cited by 9 publications

(3 citation statements)

References 24 publications

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“…Following a 15-min centrifugation at 10,000 rpm and 4 • C, the supernatant was collected and the sediment was reextracted twice with 1 mL of the extraction solvent. The supernatants from the two extracts were combined and centrifuged and then HPLC hybrid triple quadrupole-linear ion trap mass spectrometry (API 3200 Q-TRAP liquid mass combination; Thermo Fisher Scientific) was performed to analyze the phytohormone contents [53].…”

Section: Phytohormone Extraction and Measurementmentioning

confidence: 99%

The Effects of DNA Methylation Inhibition on Flower Development in the Dioecious Plant Salix Viminalis

Cheng

Peng

et al. 2019

Forests

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DNA methylation, an important epigenetic modification, regulates the expression of genes and is therefore involved in the transitions between floral developmental stages in flowering plants. To explore whether DNA methylation plays different roles in the floral development of individual male and female dioecious plants, we injected 5-azacytidine (5-azaC), a DNA methylation inhibitor, into the trunks of female and male basket willow (Salix viminalis L.) trees before flower bud initiation. As expected, 5-azaC decreased the level of DNA methylation in the leaves of both male and female trees during floral development; however, it increased DNA methylation in the leaves of male trees at the flower transition stage. Furthermore, 5-azaC increased the number, length and diameter of flower buds in the female trees but decreased these parameters in the male trees. The 5-azaC treatment also decreased the contents of soluble sugars, starch and reducing sugars in the leaves of the female plants, while increasing them in the male plants at the flower transition stage; however, this situation was largely reversed at the flower development stage. In addition, 5-azaC treatment decreased the contents of auxin indoleacetic acid (IAA) in both male and female trees at the flower transition stage. These results indicate that hypomethylation in leaves at the flower transition stage promotes the initiation of flowering and subsequent floral growth in Salix viminalis, suggesting that DNA methylation plays a similar role in vegetative–reproductive transition and early floral development. Furthermore, methylation changes during the vegetative–reproductive transition and floral development were closely associated with the biosynthesis, metabolism and transportation of carbohydrates and IAA. These results provide insight into the epigenetic regulation of carbohydrate accumulation.

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Section: Phytohormone Extraction and Measurementmentioning

confidence: 99%

The Effects of DNA Methylation Inhibition on Flower Development in the Dioecious Plant Salix Viminalis

Cheng

Peng

et al. 2019

Forests

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“…To date, many researchers have used analytical techniques such as HPLC, LC-MS/MS, and gas chromatography-mass spectrometry for the quality control of complex formulations such as TKMs, TCMs, and KMs [24][25][26][27][28]. Among the various analytical techniques, the analytical methods that include HPLC and LC-MS/MS are currently the most widely used for standardization purposes.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Analysis for Quality Control of Traditional Herbal Medicine, Gungha-Tang, Using Liquid Chromatography–Tandem Mass Spectrometry

Seo

Shin

2022

Molecules

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Gungha-tang (GHT), a traditional herbal medicine, consists of nine medicinal herbs (Cnidii Rhizoma, Pinelliae Tuber, Poria Sclerotium, Citri Unshius Pericarpium, Citri Unshius Pericarpium Immaturus, Aurantii Fructus Immaturus, Atracylodis Rhizoma Alba, Glycyrrhizae Radix et Rhizoma, and Zingiberis Rhizoma Recens). It has been used for various diseases caused by phlegm. This study aimed to develop and verify the simultaneous liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis method, using nine marker components (liquiritin apioside, neoeriocitrin, narirutin, naringin, hesperidin, neohesperidin, liquiritigenin, glycyrrhizin, and 6-shogaol) for quality control of GHT. LC–MS/MS analysis was conducted using a Waters TQ-XS system. All marker analytes were separated on a Waters Acquity UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) using gradient elution with a distilled water solution (containing 5 mM ammonium formate and 0.1% [v/v] formic acid)–acetonitrile mobile phase. LC–MS/MS multiple reaction monitoring (MRM) analysis was carried out in negative and positive ion modes of an electrospray ionization source. The developed LC–MS/MS MRM method was validated by examining the linearity, limits of detection and quantification, recovery, and precision. LOD and LOQ values of nine markers were calculated as 0.02–8.33 ng/mL and 0.05–25.00 ng/mL. The recovery was determined to be 89.00–118.08% and precision was assessed with a coefficient of variation value of 1.74–8.64%. In the established LC–MS/MS MRM method, all markers in GHT samples were detected at 0.003–16.157 mg/g. Information gathered during the development and verification of the LC–MS/MS method will be useful for the quality assessment of GHT and other herbal medicines.

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“…The availability of chemical compound databases further facilitates compound identification in TCM studies [13]. So far, many bioactive TCM constituents such as flavonoids, ginosensides, saponins, alkaloids, monoterpene glycosides and steroids can be successfully detected using different MS techniques [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Direct analysis of traditional Chinese medicines by mass spectrometry

Wong

Yao

2016

Journal of Chromatography B

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Qualitative analysis of chemical constituents in traditional Chinese medicine analogous formula cheng‐Qi decoctions by liquid chromatography–mass spectrometry

Cited by 9 publications

References 24 publications

The Effects of DNA Methylation Inhibition on Flower Development in the Dioecious Plant Salix Viminalis

The Effects of DNA Methylation Inhibition on Flower Development in the Dioecious Plant Salix Viminalis

Simultaneous Analysis for Quality Control of Traditional Herbal Medicine, Gungha-Tang, Using Liquid Chromatography–Tandem Mass Spectrometry

Direct analysis of traditional Chinese medicines by mass spectrometry

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