Qualitative analysis of the vaginal microbiota of healthy cattle and cattle with genital-tract disease

Rodrigues, Nayara Formiga; Kästle, J; Coutinho, Tarcisio José Domingos; Amorim, Aline Teixeira; Campos, Guilherme Barreto; Marques, Lucas Miranda; Timenetsky, Jorge; Farias, S T de

doi:10.4238/2015.june.12.4

Cited by 40 publications

(34 citation statements)

References 36 publications

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“…Outliers with a low sequence count and microbial diversity were removed. Mothur software (version 1.35.1; ) was then used to calculate indices of Shannon diversity and richness ( 27 ). The Ribosomal Database Project (RDP) Classifier software (version 16; ) was used to classify sequences.…”

Section: Methodsmentioning

confidence: 99%

The association between inflammation, the microbiome and urethane-induced pulmonary adenocarcinoma

Deng

Sun

et al. 2018

Oncol Lett

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Lung cancer is amongst the most common types of cancer throughout the world. The overall 5-year survival rate is ~17%. A number of studies have demonstrated that the microbiome existing within the host may affect the level of inflammation, and consequently contribute to the carcinogenesis of certain types of cancer. To investigate the role of inflammation and the microbiome in the carcinogenesis of lung cancer, an intervention study involving mice, including a control group (C; n=5), a urethane-induced pulmonary adenocarcinoma group (U; n=5) and a prebiotics intervention group (P; n=5) was carried out. This pulmonary adenocarcinoma model was reviewed, and incidences of the disease were identified using histopathology. The levels of the inflammatory cytokines nuclear factor κB (NF-κB), tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β) and IL-6 in the sera samples were measured using an ELISA technique. In addition, high-throughput sequencing of the 16S ribosomal RNA gene segment was used to analyze the species present in the microbiome of the lower airways and intestinal tracts of mice. The results demonstrated that groups P and U exhibited altered histopathology and the development of lung adenocarcinoma tumors, but no differences were observed between the groups. The level of inflammation, determined by measuring the levels of NF-κB, TNF-α, IL-1β and IL-6 inflammatory cytokines, was significantly lower in group P compared with group U (P<0.05), and was significantly higher in group P compared with group C (P<0.05). Overall, the microbiomes of the lower respiratory and intestinal tracts did not change markedly among the 3 groups, in terms of the size of colonies and Shannon diversity indices. However, at a family and operational taxonomic unit (OTU) level, certain microbiota were altered. For example, the abundance of the Clostridiales and Lachnospiraceae families was lower in the lung and intestinal tracts subsequent to urethane-induced treatment compared with in the control group (P<0.05), and the level of abundance of the Clostridiales family increased to similar levels within the control group (P<0.05), when prebiotics were administered. The levels of abundance of the S24-7, Bacteroidales and Firmicutes families were higher in the intestinal tract compared with the control group (P<0.05), and following treatment with prebiotics, the levels of abundance of these families decreased to similar levels observed in the control group (P<0.05). In conclusion, inflammation and the microbiome serve important roles in the carcinogenesis of lung cancer. Additionally, prebiotics may increase the efficacy of lung cancer treatment by modulating levels of inflammation and the composition of the microbiome. The associations between inflammation, the microbiome and lung cancer require attention.

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Section: Methodsmentioning

confidence: 99%

The association between inflammation, the microbiome and urethane-induced pulmonary adenocarcinoma

Deng

Sun

et al. 2018

Oncol Lett

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“…Several studies have previously described the microbiota of the cattle vagina 56 – 60 , skin 61 , and colostrum 62 . The microbiota determined in our study are each consistent with those reported previously.…”

Section: Discussionmentioning

confidence: 99%

Biogeographical Differences in the Influence of Maternal Microbial Sources on the Early Successional Development of the Bovine Neonatal Gastrointestinal tract

Yeoman

Ishaq

Bichi

et al. 2018

Sci Rep

134

159

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The impact of maternal microbial influences on the early choreography of the neonatal calf microbiome were investigated. Luminal content and mucosal scraping samples were collected from ten locations in the calf gastrointestinal tract (GIT) over the first 21 days of life, along with postpartum maternal colostrum, udder skin, and vaginal scrapings. Microbiota were found to vary by anatomical location, between the lumen and mucosa at each GIT location, and differentially enriched for maternal vaginal, skin, and colostral microbiota. Most calf sample sites exhibited a gradual increase in α-diversity over the 21 days beginning the first few days after birth. The relative abundance of Firmicutes was greater in the proximal GIT, while Bacteroidetes were greater in the distal GIT. Proteobacteria exhibited greater relative abundances in mucosal scrapings relative to luminal content. Forty-six percent of calf luminal microbes and 41% of mucosal microbes were observed in at-least one maternal source, with the majority being shared with microbes on the skin of the udder. The vaginal microbiota were found to harbor and uniquely share many common and well-described fibrolytic rumen bacteria, as well as methanogenic archaea, potentially indicating a role for the vagina in populating the developing rumen and reticulum with microbes important to the nutrition of the adult animal.

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“…Studies on the bovine microbiota have gained importance in the last 5 years with the characterization of the microbiota of the rumen [ 21 – 24 ], the complete gastrointestinal tract [ 25 ], the nasopharynx [ 26 ], milk [ 27 – 29 ], the teat [ 30 ] and the vagina and/or uterus [ 31 – 34 ]. In contrast to previous studies that focused on implicating specific bacteria from bovine abortion material by bacterial culture and targeted molecular detection [ 4 , 6 , 35 – 38 ], we studied the total bacterial microbiota present in abortion material from cattle using a 16S rRNA amplicon sequencing approach.…”

Section: Discussionmentioning

confidence: 99%

Amplicon sequencing of bacterial microbiota in abortion material from cattle

Vidal

Kegler

Posthaus

et al. 2017

Vet Res

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Abortions in cattle have a significant economic impact on animal husbandry and require prompt diagnosis for surveillance of epizootic infectious agents. Since most abortions are not epizootic but sporadic with often undetected etiologies, this study examined the bacterial community present in the placenta (PL, n = 32) and fetal abomasal content (AC, n = 49) in 64 cases of bovine abortion by next generation sequencing (NGS) of the 16S rRNA gene. The PL and AC from three fetuses of dams that died from non-infectious reasons were included as controls. All samples were analyzed by bacterial culture, and 17 were examined by histopathology. We observed 922 OTUs overall and 267 taxa at the genus level. No detectable bacterial DNA was present in the control samples. The microbial profiles of the PL and AC differed significantly, both in their composition (PERMANOVA), species richness and Chao-1 (Mann–Whitney test). In both organs, Pseudomonas was the most abundant genus. The combination of NGS and culture identified opportunistic pathogens of interest in placentas with lesions, such as Vibrio metschnikovii, Streptococcus uberis, Lactococcus lactis and Escherichia coli. In placentas with lesions where culturing was unsuccessful, Pseudomonas and unidentified Aeromonadaceae were identified by NGS displaying high number of reads. Three cases with multiple possible etiologies and placentas presenting lesions were detected by NGS. Amplicon sequencing has the potential to uncover unknown etiological agents. These new insights on cattle abortion extend our focus to previously understudied opportunistic abortive bacteria.

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Qualitative analysis of the vaginal microbiota of healthy cattle and cattle with genital-tract disease

Cited by 40 publications

References 36 publications

The association between inflammation, the microbiome and urethane-induced pulmonary adenocarcinoma

The association between inflammation, the microbiome and urethane-induced pulmonary adenocarcinoma

Biogeographical Differences in the Influence of Maternal Microbial Sources on the Early Successional Development of the Bovine Neonatal Gastrointestinal tract

Amplicon sequencing of bacterial microbiota in abortion material from cattle

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