Qualitative and Quantitative Analysis of Triterpene Saponins from Tea Seed Pomace (Camellia oleifera Abel) and Their Activities against Bacteria and Fungi

Zhang, Xin Fu; Yang, S. L.; Han, Ying; Жао, Леи; Lu, Gui Long; Xia, Tao; Gao, Lei

doi:10.3390/molecules19067568

Cited by 56 publications

(39 citation statements)

References 32 publications

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“…Therefore, it is believed that drugs that exhibit antioxidant activities can prove useful for the treatment of silica-induced lung injury. Consistent with this, C. oleifera seeds have been reported to contain a number of antioxidant molecules and around 70 different saponins, several terpenoids, phenolics and flavonoids have been identified from it (Zhang et al, 2014). Therefore, in the current study the influence of C. oleifera seed extract (CSE) was for the first time evaluated in a rat model of pulmonary fibrosis developed by endotracheal silica.…”

Section: Introductionmentioning

confidence: 72%

Protective Effect of Camellia Oleifera Abel. On Silica-Induced Pulmonary Fibrosis in Rats

Tian¹,

Liu²,

Yang³

et al. 2017

Afr J Tradit Complement Altern Med

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Background: Camellia oleifera Abel. belongs to the family Theaceae and genus Camellia. It is commonly cultivated in southern China. The seeds of C. oleifera have been reported to exhibit a diversity of pharmacological activities which include, but are not limited to, antioxidant, anti-cancer and antimicrobial. Pulmonary fibrosis is one of lethal causes of mortality across the globe and accretion of considerable amount of reactive oxygen species (ROS) in lungs have been implicated in the onset of this disease. Given the known antioxidant activity of C. oleifera seed extract (CSE), the present study was designed to evaluate the influence of CSE on the silica-induced pulmonary fibrosis rat models. Materials and Methods: Protective effect of CSE was determined in silica-induced pulmonary fibrosis rat models. Malondialdehyde (MDA), hydroxyproline (HP) and superoxide dismutase 2 (SOD-2) activity were determined by standard biochemical assays. Histopathological analysis was carried out by H and E staining. Phyto-constituents of CSE were identified by LC/MS analysis. Results: The results of this study indicated that CSE lowered the MDA and hydroxyproline content in silica-treated rats. Additionally, CSE also caused a significant increase in the expression of SOD-2 leading to scavenging of ROS. Hematoxylin and eosin (H and E) staining of lung tissue sections revealed that CSE maintained the integrity of parenchymatous cells of lungs and prevented the development of pulmonary fibrosis. To gain insights about the phytochemical constituents of CSE, LC/MS analysis was carried out and several antioxidant phenolics and flavonoids were tentatively identified. Conclusion: Taken together, we conclude that CSE prevents development of pulmonary fibrosis and the protective effect of CSE may be due to its ability to induce SOD-2 expression and due to the presence antioxidant phytoconstituents.

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Section: Introductionmentioning

confidence: 72%

Protective Effect of Camellia Oleifera Abel. On Silica-Induced Pulmonary Fibrosis in Rats

Tian¹,

Liu²,

Yang³

et al. 2017

Afr J Tradit Complement Altern Med

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“… developed HPLC–UVD at a wavelength of 210 nm for the quantification of ursolic acid and oleanic acid isolated from the leaves of Plumeria obtusa L. They selected the mobile phase of acetonitrile/water (85:15, v/v), which offered the best peak resolution. Meanwhile, LC–UV–ELSD was used to establish the method to qualitatively analyze triterpene saponins obtained from Camellia oleifera Abel, and the purified total saponins was found to exhibit inhibitory effects against the tested bacteria . The UV detector is also the most popular option to determine the content of pentacyclic triterpenoids.…”

Section: Discussionmentioning

confidence: 99%

Techniques for the analysis of pentacyclic triterpenoids in medicinal plants

Bing

et al. 2017

J of Separation Science

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Triterpenes are a major class of chemical compounds found in natural plants and can be categorized into acyclic triterpenoids, monocyclic triterpenoids, tricyclic triterpenoids, tetracyclic triterpenoids, and pentacyclic triterpenoids. Among them, pentacyclic triterpenoids have gained more extensive attention due to their biological activities, including anti-inflammation, antibacterial, antioxidation, antitumor, anti-HIV, hepatoprotection, and immunological adjuvant properties. In this review, we summarize the extraction and analytical methods for pentacyclic triterpenoids, where more than 56 triterpenes from 49 kinds of plants were involved. The analysis methods include gas chromatography, liquid chromatography, capillary electrophoresis, thinlayer chromatography, supercritical fluid chromatography, NMR spectroscopy, and X-ray spectroscopy. This review provides valuable reference for the determination of pentacyclic triterpenoids in medicinal plants.

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“…In our experiment, the higher ergosterol concentrations but lower microbial respiration and leaf processing in treatment BF than in BFP might be due to a high proportion of empty fungal hyphae. Due to nutrient depletion or inhibitory substances released from the leaf tissue, cytoplasm starts to move within fungal hyphae to more favourable sites leaving behind empty hyphae, that is membranes containing ergosterol (Klein & Paschke, 2004;Zhang et al, 2014). Phagotrophic protists may thus affect the microenvironment surrounding fungal hyphae and promote cytoplasm-filled hyphae and the efficiency of leaf litter processing.…”

Section: Protists Induced Changes In Fungal Growth and Efficiency Of mentioning

confidence: 99%

Phagotrophic protists are a key component of microbial communities processing leaf litter under contrasting oxic conditions

2015

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Summary The transformation of leaf litter, a key process in aquatic systems, is known to be reduced with decreasing oxygen concentrations, mainly due to lower abundance of and/or less active shredding macroinvertebrates. Aquatic fungi and phagotrophic protists involved in leaf litter processing can tolerate low oxygen, but little is known about their role in leaf litter processing under these conditions. We aimed to unravel the importance of phagotrophic protists within microbially mediated leaf litter processing under contrasting oxic conditions. We hypothesised that respiration, abundance of aquatic bacteria and biomass of aquatic fungi, and thus leaf litter processing, are enhanced in the presence of phagotrophic protists, both under normoxic and low oxic conditions. Lower leaf processing was expected under low oxic than under normoxic conditions. In microcosms, oxygen concentration was adjusted to either normoxic or low. For a total of 105 days, leaf litter was cocultivated with three microbial communities: (i) a multispecies bacterial community enriched from stream leaf litter, (ii) the aquatic fungus Heliscus lugdunensis added to the bacterial community and (iii) the phagotrophic protist Glaucoma scintillans added to the bacteria–fungi community. Oxic condition had no significant effect on microbially mediated leaf mass loss. The leaf mass loss was faster in the presence of the aquatic fungus and further accelerated by adding the phagotrophic protist. After 105 days, leaf mass remaining approximated 64–79%, 57–68% and 55–61% of initial leaf mass in the bacteria, bacteria–fungi, and bacteria–fungi–phagotrophic protist communities, respectively. Under both oxic conditions, the lower leaf toughness indicated that the aquatic fungus had the potential to process leaf structural components more efficiently than bacteria alone. The combination of lower ergosterol concentrations and enhanced leaf mass loss indicated that phagotrophic protists stimulated the efficiency of leaf processing by the microbial community. Under normoxic conditions, leaf‐associated respiration increased when successively adding an aquatic fungus and a phagotrophic protist to bacterial communities, which matches the faster leaf litter processing in these treatments. Under low oxic conditions, respiration of all three microbial communities was comparable. Thus, enhanced leaf litter processing in treatments with aquatic bacteria, fungi and phagotrophic protists was presumably caused either by other metabolic pathways such as fermentation and anaerobic respiration, or by changes in community composition and growth efficiency of the microbial community. Microbial communities composed of aquatic bacteria, fungi and phagotrophic protists play a crucial role in leaf litter processing under a range of oxic conditions. Although processing of leaf litter by microbial communities alone is slower than in the presence of shredding invertebrates, the interaction of aquatic bacteria, fungi and phagotrophic protists can modulate leaf quality and subsequen...

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Qualitative and Quantitative Analysis of Triterpene Saponins from Tea Seed Pomace (Camellia oleifera Abel) and Their Activities against Bacteria and Fungi

Cited by 56 publications

References 32 publications

Protective Effect of Camellia Oleifera Abel. On Silica-Induced Pulmonary Fibrosis in Rats

Protective Effect of Camellia Oleifera Abel. On Silica-Induced Pulmonary Fibrosis in Rats

Techniques for the analysis of pentacyclic triterpenoids in medicinal plants

Phagotrophic protists are a key component of microbial communities processing leaf litter under contrasting oxic conditions

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