QUALITATIVE ASSESSMENT OF GENOTOXICITY USING RANDOM AMPLIFIED POLYMORPHIC DNA: COMPARISON OF GENOMIC TEMPLATE STABILITY WITH KEY FITNESS PARAMETERS IN DAPHNIA MAGNA EXPOSED TO BENZO[a]PYRENE

Atienzar, Franck; Conradi, Mercedes; Evenden, Andrew J.; Jha, Awadhesh N.; Depledge, Michael H.

doi:10.1897/1551-5028(1999)018<2275:qaogur>2.3.co;2

Cited by 52 publications

(79 citation statements)

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“…In the present study, DNA damage was estimated using RAPD profiles that reflect DNA effects in treated plant seedlings. Previous studies have shown that changes in band patterns observed in DNA ‘fingerprint’ analyses reflect DNA alterations from single base changes (point mutations) to complex chromosomal rearrangements [18,45,46]. Similarly, in the present study, DNA damage induced by heavy metals was reflected by changes in RAPD profiles; disappearance of bands and appearance of new PCR products occurred in the profiles generated by exposed plants.…”

Section: Discussionsupporting

confidence: 76%

Application of random amplified polymorphic DNA (RAPD) to detect the genotoxic effect of heavy metals

Enan

2006

Biotech and App Biochem

100

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This paper presents the results of a study on the influence of lead, copper, manganese and cadmium on DNA integrity in plant cells. Plants, as biological indicators, can measure the potential effects of pollutants when they are used to measure effects of heavy metals. The genotoxicity of heavy metals in kidney-bean (Phaseolus vulgaris) seedlings was subjected to RAPD (random amplified polymorphic DNA) analysis. An RAPD 'fingerprinting' technique was used to detect DNA damage in the kidney-bean seedlings treated with two selected heavy metals at concentrations of 150 and 350 mg x l(-1). Polymorphisms became evident as the presence and/or absence of DNA fragments in treated samples compared with the untreated one. At 350 mg x l(-1), a high number of both missing bands and new amplified fragment were observed. Results suggested that a qualitative measure reflecting changes in RAPD profiles were significantly affected at higher concentrations (350 mg x l(-1)) of the tested heavy metals. A total of 467 RAPD fragments in RAPD profiles were detected by using six random primers (decamers) and 224 of these fragments showed polymorphism. There was a distinct distance between the band patterns of treated plants and the control samples when the cluster method was applied. In addition, the result derived from numerical analysis revealed a considerable distance between the band pattern of the plant samples treated with 350 mg x l(-1) heavy metals and the control sample. Finally, a comparison between untreated and treated genomes shows that RAPD analysis can be used to evaluate how the environmental pollutants modify the structure of DNA in living organisms.

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Section: Discussionsupporting

confidence: 76%

Application of random amplified polymorphic DNA (RAPD) to detect the genotoxic effect of heavy metals

Enan

2006

Biotech and App Biochem

100

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“…''a'' is the number of band losses and appearance of new bands in DNA profiles and ''n'' is the number of bands detected in control DNA profiles. Calculated GTS values were compared with control GTS values (set to 100%) (Atienzar et al, 1999).…”

Section: Rapd-pcr Testmentioning

confidence: 99%

Investigation of flurbiprofen genotoxicity and cytotoxicity in rat bone marrow cells

Timocin

İla

2014

Drug and Chemical Toxicology

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This study was performed to investigate cytogenetic effects of NSAID flurbiprofen which was used as active ingredient in some analgesic, antipyretic and anti-inflammatory drugs. Genotoxic effect of flurbiprofen was investigated using in vivo chromosome aberration (CA) test and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) test. Also, oxidative stress potential of flurbiprofen was determined by measuring total oxidant and antioxidant level which occurred with flurbiprofen treatment in rat peripheral blood. For these purposes, rats were treated with three concentrations of flurbiprofen (29.25, 58.50 and 117 mg/kg, body weight) in single dose at two different treatment periods (12 and 24 h). According to the results, flurbiprofen did not affect chromosome aberrations in rat bone marrow cells with CA test. In RAPD-PCR test, polymorphic bands were unaffected. Also, test substance did not change total oxidant and antioxidant status (except for 58.50 and 117 mg/kg, 12 h) and therefore it did not lead to significant increase on oxidative stress (again except 58.50 and 117 mg/kg, 12 h). However, flurbiprofen reduced to mitotic indexes and these reductions were dose-dependent for 12 h treatment. In summary, flurbiprofen did not show significant genotoxic effect. But it caused cytotoxicity in rat bone marrow cells.

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“…RAPD profiles generated from rats exposed to benzo [a] pyrene (B [a] P) revealed differences in the appearance and disappearance of bands in comparison to the RAPD profiles obtained from the control animals. RAPD methods have also been successfully used to detect DNA damage induced by radionuclides (Theodorakis and Shugart, 1997), mitomycin C (Becerril et al 1999), benzo [a] pyrene (Atienzar et al 1999;Atienzar et al 2002a, b;Castano and Becerril 2004), UV radiations, X-rays, gamma radiations (Kuroda et al 1999;Atienzar et al 2000;Jones and Kortenkamp 2000;Atak et al 2004;Hagger et al 2005), metals such as lead, manganese, cadmium and copper (Atienzar et al 2001;Liu et al 2005;Enan 2006), 17-ß estradiol (Atienzar et al 2002c).…”

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confidence: 99%

PCR based detection of furadan genotoxicity effects in rohu (Labeo rohita) fingerlings

Mohanty

Garnayak

et al. 2009

Vet Res Commun

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The present study involves the use of RAPD-PCR to evaluate the genotoxic effects of furadan in the DNA of Labeo rohita (rohu) fingerlings. Rohu fingerlings were exposed to 0.02 ppm of furadan for a total period of 96 h and samplings were done at 24, 48, 72 and 96 h. RAPD - PCR were carried out with the blood and liver DNA samples of both control and treated groups at each of the four sampling hours. A total of six selected RAPD primers were used for PCR amplification. Template stability has been taken as the measure of DNA damage caused by pesticide. The results obtained showed no significant difference in the template stability in the blood DNA of furadan treated groups at any of the four sampling hours; however, the liver DNA were able to show significant difference at 48 and 96 hours of treatment.

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QUALITATIVE ASSESSMENT OF GENOTOXICITY USING RANDOM AMPLIFIED POLYMORPHIC DNA: COMPARISON OF GENOMIC TEMPLATE STABILITY WITH KEY FITNESS PARAMETERS IN DAPHNIA MAGNA EXPOSED TO BENZO[a]PYRENE

Cited by 52 publications

References 0 publications

Application of random amplified polymorphic DNA (RAPD) to detect the genotoxic effect of heavy metals

Application of random amplified polymorphic DNA (RAPD) to detect the genotoxic effect of heavy metals

Investigation of flurbiprofen genotoxicity and cytotoxicity in rat bone marrow cells

PCR based detection of furadan genotoxicity effects in rohu (Labeo rohita) fingerlings

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