Qualitative composition of carotenoids, catalase and superoxide dismutase activities in tissues of the bivalve mollusc Anadara inaequivalvis (Bruguiere, 1789)

Солдатов, А. А.; Gostyukhina, O. L.; Borodina, A. V.; Головина, И. В.

doi:10.1134/s0022093013040026

Cited by 20 publications

(10 citation statements)

References 25 publications

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“…CAT plays an important role in the defense against oxidative stress through the removal of H 2 O 2 which is the major precursor of hydroxyl radical (Soldatov et al, 2013). In our study, significant increase in CAT activity was observed in both organs suggesting that CAT acts as a defense mechanism against MPs.…”

Section: Modulation Of Anti-oxidant Enzymes and Ros Production By Comsupporting

confidence: 60%

Tissue-Specific Biomarker Responses in the Blue Mussel Mytilus spp. Exposed to a Mixture of Microplastics at Environmentally Relevant Concentrations

Revel

Lagarde

Perrein-Ettajani

et al. 2019

Front. Environ. Sci.

122

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The impact of a microplastic (MP) mixture composed of polyethylene (PE) and polypropylene (PP) plastic particles, prepared from commercially available products, was evaluated in blue mussels Mytilus spp. exposed to three environmentally relevant concentrations: 0.008 µg L −1 (low), 10 µg L −1 (medium), and 100 µg L −1 (high). Organisms were exposed for 10 days followed by 10 days of depuration in clean seawater under controlled laboratory conditions. The evaluation of MP effects on mussel clearance rate, tissue structure, antioxidant defenses, immune and digestive parameters, and DNA integrity were investigated while the identification of plastic particles in mussel tissues (gills, digestive gland, and remaining tissues), and biodeposits (feces and pseudofaeces) was performed using infrared microscopy (µFT-IR). Results showed the presence of MPs only in the digestive gland of mussels exposed to the highest tested concentration of MPs with a mean of 0.75 particle/mussel (after the 10 days of exposure). In biodeposits, PE and PP particles were detected following exposure to all tested concentrations confirming the ingestion of MPs by the organisms. A differential response of antioxidant enzyme activities between digestive gland and gills was observed. Significant increases in superoxide dismutase (SOD) and catalase (CAT) activities were measured in the digestive gland of mussels exposed to the low (0.008 µg L −1) and medium (10 µg L −1) concentrations of MPs and in the gills from mussels exposed to the highest concentration (100 µg L −1) of MPs that could be indicative of a change in the redox balance. Moreover, an increase in acid phosphatase activity was measured in hemolymph of mussels exposed to 0.008 and 10 µg L −1 concentrations. No significant difference was observed in the clearance rate, and histopathological parameters between control and exposed mussels. This study brings new insights on the potential sublethal impacts of MPs at environmentally relevant concentrations in marine bivalves.

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Section: Modulation Of Anti-oxidant Enzymes and Ros Production By Comsupporting

confidence: 60%

Tissue-Specific Biomarker Responses in the Blue Mussel Mytilus spp. Exposed to a Mixture of Microplastics at Environmentally Relevant Concentrations

Revel

Lagarde

Perrein-Ettajani

et al. 2019

Front. Environ. Sci.

122

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“…As suggested by Hedegaard et al (2006), however, the type of polyenes is influenced by food. The ingestion of pigment-enriched microalgae potentially leads to an accumulation of pigments in mollusk tissues and the shell (Soldatov et al, 2013). On the other hand, it has been argued that polyenes do not generate from food sources like other pigments (i.e., carotenoids), but they are locally synthesized (Karampelas et al, 2009).…”

Section: Environmental Influence On Shell Microstructurementioning

confidence: 99%

“…Given the high phenotypic variation in pigmentation among and within mollusk species, it has been proposed that coloration does not have a primary function as an adaptive tool (i.e., camouflage, warning signaling) as in other animals (Seilacher, 1972;Evans et al, 2009). This, in turn, can indicate a certain degree of influence of the environment on the pigments, in particular by diet (Hedegaard et al, 2006;Soldatov et al, 2013). In the current study, the effect of different dietary regimes was tested in order to explore the potential of polyenes as environmental proxy.…”

Section: A Islandica Shell Organizationmentioning

confidence: 99%

The effects of environment on <i>Arctica islandica</i> shell formation and architecture

et al. 2017

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Abstract. Mollusks record valuable information in their hard parts that reflect ambient environmental conditions. For this reason, shells can serve as excellent archives to reconstruct past climate and environmental variability. However, animal physiology and biomineralization, which are often poorly understood, can make the decoding of environmental signals a challenging task. Many of the routinely used shell-based proxies are sensitive to multiple different environmental and physiological variables. Therefore, the identification and interpretation of individual environmental signals (e.g., water temperature) often is particularly difficult. Additional proxies not influenced by multiple environmental variables or animal physiology would be a great asset in the field of paleoclimatology. The aim of this study is to investigate the potential use of structural properties of Arctica islandica shells as an environmental proxy. A total of 11 specimens were analyzed to study if changes of the microstructural organization of this marine bivalve are related to environmental conditions. In order to limit the interference of multiple parameters, the samples were cultured under controlled conditions. Three specimens presented here were grown at two different water temperatures (10 and 15 • C) for multiple weeks and exposed only to ambient food conditions. An additional eight specimens were reared under three different dietary regimes. Shell material was analyzed with two techniques; (1) confocal Raman microscopy (CRM) was used to quantify changes of the orientation of microstructural units and pigment distribution, and (2) scanning electron microscopy (SEM) was used to detect changes in microstructural organization. Our results indicate that A. islandica microstructure is not sensitive to changes in the food source and, likely, shell pigment are not altered by diet. However, seawater temperature had a statistically significant effect on the orientation of the biomineral. Although additional work is required, the results presented here suggest that the crystallographic orientation of biomineral units of A. islandica may serve as an alternative and independent proxy for seawater temperature.

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“…Previously, microstructures of other mollusk species were shown to be influenced by the environment (Hedegaard et al, 2006;Nehrke and Nouet, 2011;Soldatov et al., 2013;Stemmer and Nehrke, 2014;Milano et al, 2017a). For instance, water temperature was identified as the major factor controlling size and shape of individual biomineral units in the non-denticular composite prismatic microstructure of Cerastoderma edule (Milano et al, 2017b).…”

Section: Controls On Microstructural Characteristics Of a Islandica mentioning

confidence: 99%

“…For example, the size and elongation of individual biominerals in Cerastoderma edule (Milano et al, 2017b) and the cyclical changes in thickness of the outer layer of Scapharca broughtonii (Nishida et al, 2012) shells are related to temperature changes. Moreover, the relationship between food conditions and microstructure have lately been explored; some studies reported an accumulation of pigments in mollusk shells due to the ingestion of pigment-enriched microalgae (polyenes; Hedegaard et al 2006;Soldatov et al., 2013), while others argued that diets do not influence shell pigment composition, and that polyenes are likely species-specific and habitat independent (Nehrke and Nouet, 2011;Stemmer and Nehrke, 2014;Milano et al, 2017a). The study of shell microstructure can therefore help to develop alternative techniques to reconstruct environmental variables from bivalve shells (Milano et al, 2017a).…”

Section: Introductionmentioning

confidence: 99%

Environmental and biological factors influencing trace elemental and microstructural properties of Arctica islandica shells

Ballesta-Artero

Zhao

Milano

et al. 2018

Science of The Total Environment

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Long-term and high-resolution environmental proxy data are crucial to contextualize current climate change. The extremely long-lived bivalve, Arctica islandica, is one of the most widely used paleoclimate archives of the northern Atlantic because of its fine temporal resolution. However, the interpretation of environmental histories from microstructures and elemental impurities of A. islandica shells is still a challenge. Vital effects (metabolic rate, ontogenetic age, and growth rate) can modify the way in which physiochemical changes of the ambient environment are recorded by the shells. To quantify the degree to which microstructural properties and element incorporation into A. islandica shells is vitally or/and environmentally affected, A. islandica specimens were reared for three months under different water temperatures (3, 8 and 13 °C) and food concentrations (low, medium and high). Concentrations of Mg, Sr, Na, and Ba were measured in the newly formed shell portions by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). The microstructures of the shells were analyzed by Scanning Electron Microscopy (SEM). Shell growth and condition index of each specimen were calculated at the end of the experimental period. Findings indicate that no significant variation in the morphometric characteristics of the microstructures were formed at different water temperatures or different food concentrations. Shell carbonate that formed at lowest food concentration usually incorporated the highest amounts of Mg, Sr and Ba relative to Ca (except for Na) and was consistent with the slowest shell growth and lowest condition index at the end of the experiment. These results seem to indicate that, under food limitation, the ability of A. islandica to discriminate element impurities during shell formation decreases. Moreover, all trace element-to‑calcium ratios were significantly affected by shell growth rate. Therefore, physiological processes seem to dominate the control on element incorporation into A. islandica shells.

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Qualitative composition of carotenoids, catalase and superoxide dismutase activities in tissues of the bivalve mollusc Anadara inaequivalvis (Bruguiere, 1789)

Cited by 20 publications

References 25 publications

Tissue-Specific Biomarker Responses in the Blue Mussel Mytilus spp. Exposed to a Mixture of Microplastics at Environmentally Relevant Concentrations

Tissue-Specific Biomarker Responses in the Blue Mussel Mytilus spp. Exposed to a Mixture of Microplastics at Environmentally Relevant Concentrations

The effects of environment on <i>Arctica islandica</i> shell formation and architecture

Environmental and biological factors influencing trace elemental and microstructural properties of Arctica islandica shells

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Qualitative composition of carotenoids, catalase and superoxide dismutase activities in tissues of the bivalve mollusc Anadara inaequivalvis (Bruguiere, 1789)

Cited by 20 publications

References 25 publications

Tissue-Specific Biomarker Responses in the Blue Mussel Mytilus spp. Exposed to a Mixture of Microplastics at Environmentally Relevant Concentrations

Tissue-Specific Biomarker Responses in the Blue Mussel Mytilus spp. Exposed to a Mixture of Microplastics at Environmentally Relevant Concentrations

The effects of environment on &lt;i&gt;Arctica islandica&lt;/i&gt; shell formation and architecture

Environmental and biological factors influencing trace elemental and microstructural properties of Arctica islandica shells

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The effects of environment on <i>Arctica islandica</i> shell formation and architecture