Qualitative parameters of the piapara semen (Leporinus elongatus Valenciennes, 1850)

Abstract

Qualitative parameters of piapara semen (Leporinus elongatus) were evaluated before and after hormonal induction with carp pituitary extract at 2.5 mg.kg -1 of live weight. The progressive motility, the spermatic vigor and the lifetime of the spermatozoa were higher before the hormonal induction (P > 0.05). The percentage of normal spermatozoa and spermatozoa with secondary pathologies did not differ (P > 0.05) between treatments: before induction (44.0 and 44.4%, respectively) and after-induction (44.3 and 46.7%, respectively). However, the percentage of primary pathologies was higher (P < 0.05) for the semen collected before induction than for the semen collected after induction; the estimates were 12.2 and 8.0%, respectively. The most frequent pathologies were the taillessness with the frequencies of 27.4 and 36.3% followed by the headlessness for which the estimates were 10.1 and 3.9%, before and after induction respectively. The semen collected before the hormonal induction presented better qualitative parameters.Keywords: spermatic cells, spermatic morphology, fish. Parâmetros qualitativos do sêmen de piapara (Leporinus elongatus Valenciennes, 1850) ResumoOs parâmetros qualitativos do sêmen de piaparas (Leporinus elongatus) foram avaliados antes e após a indução hormonal com o extrato de hipófise de carpa na dosagem 2,5 mg.kg -1 de peso vivo. A motilidade progressiva, o vigor espermático e o tempo de vida dos espermatozóides apresentaram valores superiores (P < 0,05) no sêmen coletado antes da indução hormonal. Já a estimativa da porcentagem de espermatozóides normais ou com patologias leves, na pré-indução (44,0 e 44,4%, respectivamente) e na pós-indução hormonal (44,3 e 46,7%, respectivamente) não diferiram (P > 0,05) entre os tratamentos. Porém a estimativa de patologias graves foi maior (P < 0,05) no sêmen de pré-indução do que no pós-induzido com 12,2 e 8%, respectivamente. A patologia mais freqüente foi cabeça solta com 27,4 e 36,3% seguida por cauda solta com 10,1 e 3,9%, antes e após a indução hormonal respectivamente. De acordo com os resultados, o sêmen de piapara coletado na pré-indução hormonal apresentou os melhores parâmetros qualitativos.Palavras-chave: células espermáticas, morfologia espermática, peixes.

Ovopel and Carp Pituitary Extract as Spawning Inducers in Males of the Amazon Catfish Leiarius marmoratus (Gill, 1970)

“…These tests are implemented in the PROC GLM and PROC GENMOD procedures, respectively. MiraLópez et al (2010) for the same specie (89.0%) and for other species (Streit Jr et al 2008;Povh et al 2010;Maria et al 2011;Streit Jr et al 2012). In addition, no reference range of sperm motility was available for L. marmoratus in Brazil.…”

“…Sperm count was determined by diluting 5.0 µL of the semen sample in 25 mL buffered formal saline (1:5000) and spermatozoa were counted in a Neubauer chamber with 10 µL diluent (semen + buffered formal saline) under a light microscope (20X). Sperm morphology was evaluated in the smears of the diluents (semen + buffered formal saline) stained with Rose Bengal as described by Streit Jr et al (2008). The microscope slide containing the smears was dried and examined under a phase-contrast microscope at 40X magnification.…”

“…A significant difference was only in the frequency of loose heads, which was lower in the treatments with 0.4 and 0.6 Ovopel pellet/kg live weight (10.32 and 41.67%, respectively). A number of studies have shown high frequencies of secondary sperm defects in fish, particularly loose heads and tails (Streit Jr et al 2008;Maria et al 2011). These high frequencies of secondary abnormalities might be related to the procedure of smear preparation or semen collection, environmental temperature, diseases, animal feeding and sperm duct problems (Herman et al 1994).…”

Semen characteristics of the amazonian jundiá (Leiarius marmoratus Gill, 1870)

“…Other studies report 90% (KAVAMOTO et al, 1999) and 97% (MILIORINI et al, 2011) normal sperm in Prochilodus lineatus, 64% in Leporinus elongatus (STREIT Jr. et al 2008b), 57% in the dorado Salminus maxillosus (STREIT Jr. et al 2008a), 53% in the cachara Pseudoplatystoma reticulatum (STREIT Jr. et al 2012) and 16% in the Nile tilapia Oreochromis niloticus (MATAVELI et al 2007). The variation of normal semen percentage found among the species is influenced by several factors such as the nutritional status of the fish, size, age, and time of the year (VIVEIROS; GODINHO, 2009;SANCHES et al, 2011;NAVARRO et al, 2014).…”

QUALIDADE DE GAMETAS COM A TÉCNICA DE ENDOSCOPIA EM CURIMBA (Prochilodus lineatus)

“…Porém, estes autores descreveram que os percentuais elevados deste tipo de anormalidades podem estar relacionados ao procedimento de preparação e coleta do sêmen (Streit-Jr et al, 2008).…”