Quality and lipid composition of spermatozoa in rabbits fed DHA and vitamin E rich diets

Gliozzi, T.M.; Zaniboni, L.; Maldjian, A.; Luzi, F.; Maertens, Luc; Cerolini, S.

doi:10.1016/j.theriogenology.2008.10.022

Cited by 61 publications

(42 citation statements)

References 48 publications

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“…Unfortunately, the direct effect of dietary cholesterol intake on the endogenous cholesterol concentration in sperm plasma membranes of wild-type animals has not been demonstrated, as it was for fatty acids (60)(61)(62). Cholesterol content in sperm traveling through the epididymal tract may be regulated by human epididymisspecifi c proteins (HE1-HE6), initially described in the human epididymis ( 63 ).…”

Section: Other Sterol Intermediates In Male Reproductionmentioning

confidence: 99%

Sterols in spermatogenesis and sperm maturation

Keber

Rozman

Horvat

2013

Journal of Lipid Research

106

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Section: Other Sterol Intermediates In Male Reproductionmentioning

confidence: 99%

Sterols in spermatogenesis and sperm maturation

Keber

Rozman

Horvat

2013

Journal of Lipid Research

106

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“…Sabe-se que a viscosidade do meio interfere na livre circulação dos espermatozoides pelo plasma seminal (CASTELLINI et al, 2000). Estudos recentes indicam que búfalos submetidos à dieta rica em lipídios apresentaram elevação das taxas séricas de lipídios totais e de colesterol (SILVA et al, 2014), e que sob essas circunstâncias o plasma seminal pode incorporar maior quantidade de colesterol livre (GLIOZZI et al, 2009). Em tese, esses fatores são capazes de aumentar a viscosidade do plasma seminal e alterar a trajetória em linha reta da célula espermática.…”

Section: Pi_h342 (%) E_n (%)unclassified

A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

et al. 2014

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This research aimed to evaluate the effect of inclusion unsaturated fatty acids (UFAs) in the buffalo diet, on the sperm quality after thawing. In parallel, it was verified the degree of association among techniques to assess plasma membrane integrity (PMI) of spermatozoa. Patterns of sperm motility (subjective and computer-assisted analysis), kinetics, morphology and plasma membrane integrity were evaluated. The hypo-osmotic swelling test, nigrosin-eosin staining (E-N) and fluorescent probes propidium iodide/Hoechst 33342 (IP-H342) were adopted to evaluate PMI. The study was planned considering two experimental groups, which were evaluated in repeated measures design. over time. Eleven Murrah buffalo bulls (3.5±1.0 years, and 536.9±73.0 kg) were divided in Control Group (CONT, n=5) and Palm Oil Group (PO, n=6). Animals were fed with forage and concentrated in a controlled manner. Animals of PO received dietary supplementation containing palm oil (2% of dry matter). Dietary consumption was daily monitored and the intake level of UFAs in PO was 55.4% higher than CONT. Semen samples were collected and evaluated every 14 days. The experiment lasted 120 days, considering four periods of 30 days each (Periods I, II, III and IV) and repeated measures structure was used in statistical analysis. Significance level adopted was 5%. There was no effect of treatment on sperm motility (P>0.05), but palm oil supplementation presented a positive effect on spermatic ability to progress (derived from BCF, STR and LIN) in Period IV (P<0.05). High correlation (91%) was observed between IP-H342 and E-N techniques (Y = 0.9155 x + 2.9843, R 2 = 0.82, P≤0.05). Thus, supplementation with UFAs increased the progressivity of spermatozoa after 90 days, and had no effect on other seminal parameters. Eosin-nigrosin stain can be used efficiently as a predictor of the level of PMI when conditions for implementing fluorescent probes not present.

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“…Lipid composition is acknowledged as an important structural and functional component of spermatic cells. Rabbit sperm contains high amounts of n-6 PUFA series and lower amounts of n-3 PUFA; this is a peculiarity of the rabbit within mammals, in which n-3 PUFA prevail (Gliozzi et al, 2009). The presence of unsaturated fatty acids increases the susceptibility of spermatozoa to peroxidation, which contributes to a negative effect on semen quality (de Lamirande et al, 1997;Bansal and Bilaspuri, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effect of lycopene supplementation on semen quality and reproductive performance in rabbit

et al. 2012

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A total of 18 hybrid strain Martini male rabbits were randomly divided into 3 experimental groups. The animals in the first and second groups drank water with 0.1 and 0.5 g/L of lycopene addition (0.1 and 0.5 groups, respectively), while water without any supplement was administered to the control group rabbits (0 group) for 8 wk. Semen was collected from 18 bucks (6 animals/group) for 5 consecutive weeks. Ejaculate volume was determined by graduated test tube and sperm concentration was calculated by Neubauer chamber. Sperm motility was evaluated subjectively by a phase contrast microscope and forward progressive motility (FPM) scored 1-4 (low-high). Sperm viability was assessed by nigrosin/eosin (N/E) staining procedure. At the end of the semen evaluation period, 20 females/group were inseminated on day 11 post partum with a pool of semen collected from each experimental group. Data showed that the highest level of lycopene (0.5 group) resulted in a significantly greater volume of ejaculate and total number of sperm than in the control group (0.98 vs. 0.78 mL and 364 vs. 227; P<0.05), while sperm concentration was not affected. Lycopene addition did not significantly affect the sperm quality of fresh semen, but influenced the semen traits during storage (24 h at 5°C). In particular, the motility, FPM and viability of the 0.5 group were significantly higher (P<0.05) than those found in the 0 and 0.1 groups. No significant differences were found in reproductive performance. This study shows the positive effects of lycopene supplementation on semen production and refrigerated semen characteristics. Lycopene supplementation of drinking water could have interesting applications in field conditions, although further research into the role of this antioxidant in animal production is needed.

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Quality and lipid composition of spermatozoa in rabbits fed DHA and vitamin E rich diets

Cited by 61 publications

References 48 publications

Sterols in spermatogenesis and sperm maturation

Sterols in spermatogenesis and sperm maturation

A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

Effect of lycopene supplementation on semen quality and reproductive performance in rabbit

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