Quality assessment of platelets stored in a modified platelet additive solution with trehalose at low temperature (10 °C) andin vivoeffects on rabbit model of thrombocytopenia

Abstract

Trehalose is widely used as a cryoprotective reagent to preserve various cells. Platelet additive solution-III (PAS) has been used to maintain platelet function, benefit the virus inactivation, and extend the storage period. PAS with trehalose (PAS-III M + T) may effectively protect platelets (PLTs) at a relatively low temperature (10 °C). The apheresis PLTs from six donors were divided into two groups. Group A was stored in PAS-III M + T at 10 °C as experimental group and group B in plasma at 22 °C as control group. The samples were collected on different storage dates, and multiple parameters were determined or investigated for in vitro studies. The in vivo recovery and survival of rabbit PLTs stored in the same conditions, and then labeled with (51)Cr were measured and evaluated using a rabbit model of thrombocytopenia. Over 9 days, P-selectin expression increased significantly in a time-dependent manner in both groups (n = 6). The levels of the hypotonic shock reaction and PLT aggregation rate decreased in both groups and were significantly higher in group A than B after 1 day of storage. The lactate dehydrogenase (LDH) release and glucose (GLU) consumption increased similarly, but the levels were significantly lower in group A than B. The pH decreased significantly after 5 days of storage in group B but did not change in group A. After 5 days, the morphology of the PLTs in group B maintained a more normal shape than that of group A. The recovery and survival of PLTs stored in both groups were not significantly different (p > 0.05). The bacteria growth was not examined out in both groups for up to 5 (group A) and 9 (group B) days. Storage of PLTs in the modified PAS at low temperature was more effective in protecting PLT functions than that of standard storage method and may have the potential to decrease the risk of PLT activation and bacterial contamination.

Manufacturing variables and hemostatic function of cold‐stored platelets: a systematic review of the literature

“…Of the 19 (58%) articles in which the CSP products were WBD, 10 (53%) articles were produced by buffy coat preparations, and nine (47%) were produced via platelet‐rich plasma (PRP) preparations . The predominant anticoagulant for WBD platelets was citrate–phosphate‐dextrose, which was reported in 10 (53%) articles (Table ) .…”

“…Of these, the suspension medium was plasma alone in 19 (68%), plasma plus platelet additive solution (PAS) in 11 (39%), and both media in two articles . A total of 12 (36%) articles described supplementation of the suspension medium with additives (Table ) . Storage bag specification details were included in 21 (64%) articles, of which 20 (95%) reported use of gas‐permeable bags .…”

“…A total of 32 (97%) short‐listed articles reported a CSP storage temperature of 4°C, and one reported a temperature of 10°C, which was not considered for any grouped analyses of CSPs. Agitation of CSPs was reported in 28 (85%) articles, no agitation was used in two (6%) articles, and the remaining two (6%) articles compared agitated versus nonagitated CSPs .…”

“…Agitation of CSPs was reported in 28 (85%) articles, no agitation was used in two (6%) articles, and the remaining two (6%) articles compared agitated versus nonagitated CSPs . CSPs were monitored for bacterial contamination in seven (21%) articles …”

“…In all of the 29 (89%) articles in which RTPs were used as a comparator to CSPs, the RTPs were stored at 22 ± 2°C with agitation, but collection and storage methods varied . However, in 28 articles, the collection and storage methods for RTPs were the same as the CSP test product.…”