Quality assurance for molecular testing in plant health.

Chabirand, A.; Anthoine, G.; Fox, Adrian; Laurenson, L.

doi:10.1079/9781780641478.0173

Cited by 2 publications

(2 citation statements)

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“…As early as 1934, the movement of viruses had been studied in tomato using the related tobamovirus, TMV (Samuel, 1934). This Test validation studies tend to focus on the analytical sensitivity and analytical specificity of a test, determining the limit of detection and characteristics of the test (Chabirand et al, 2016;Roenhorst et al, 2018). Currently, many laboratories implement diagnostic cut-offs, that is, a decision threshold dependent on validation data supporting a positive/negative inference.…”

Section: Discussionmentioning

confidence: 99%

Detection of tomato brown rugose fruit virus is influenced by infection at different growth stages and sampling from different plant parts

Skelton,

van Gemert,

Fowkes

et al. 2023

Plant Pathology

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Since the first report of the virus in 2014, tomato brown rugose fruit virus (ToBRFV) has spread widely through Europe, the Americas and Asia. Within Europe there is currently a requirement for annual surveillance for the virus. However, little is known about the relative impact of sampling strategy with respect to timing of infection and the detection of virus from different plant parts. To test reliably for ToBRFV in crops of unknown infection status, this issue needed to be addressed. To do this, two different approaches were followed: (1) inoculation experiments were conducted at two institutes to look at the relative effects of time of infection, plant parts, cropping season and cultivar on detection of the virus; and (2) sampling and testing various plant parts were carried out during active outbreaks from two tomato production sites in the Netherlands to look at the effect of sampling plant parts on detection of the virus. In inoculation experiments, the greatest impact on detection was timing of infection, with plants infected early in the growth cycle showing a predictable development of infection. In plants infected later, infection was detectable in sepals (calyx) earlier than in older leaves. In the studies carried out on commercial crops during ToBRFV outbreaks, the highest virus concentrations were obtained from testing sepals and young leaves. Thus, in a young crop where sepals and fruit are not yet developed, sampling should focus on the young leaves; in a mature crop it may be better to sample sepals and/or fruit.

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Section: Discussionmentioning

confidence: 99%

Detection of tomato brown rugose fruit virus is influenced by infection at different growth stages and sampling from different plant parts

Skelton,

van Gemert,

Fowkes

et al. 2023

Plant Pathology

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“…Since the ultimate purpose of quality management is to provide a result in which both the laboratory and its client can have confidence (Chabirand et al, 2016), there may be alternative approaches to meet such necessary quality requirements. The reported result will rarely depend on the results of a single test, and secondary testing, using methods based on alternative biological principles, is routinely applied in plant virology to confirm the presence and identity of the virus.…”

Section: Introductionmentioning

confidence: 99%

Ensuring validation in diagnostic testing is fit for purpose: a view from the plant virology laboratory

Roenhorst¹,

Krom²,

Fox

et al. 2018

EPPO Bulletin

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Plant diagnostic laboratories are facing increasing demands for accreditation of their testing activities from both private and public organizations. For diagnostics, validating tests for the intended use is one of the key elements of accreditation. Thus far, validation has mainly focused on tests with a fixed scope, i.e. a test for organism X in matrix Y by method Z, suitable for the analysis of targeted samples. In many laboratories, however, targeted samples only form a minority of the samples. The majority consists of symptomatic (non‐targeted) samples including a huge variety of pest, host and matrix combinations. Since it is impossible to validate all tests used for the analysis of these non‐targeted samples, ensuring the reliability of the final results needs a different approach. This paper describes such an approach for diagnostic testing in plant virology, where the extent of validation of a test is determined based on the risks of a false result within the context of the diagnostic process. Examples illustrate how this risk‐based system approach relies on a tight inter‐relationship of validation, expertise and quality assurance within the diagnostic laboratory.

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Quality assurance for molecular testing in plant health.

Cited by 2 publications

References 0 publications

Detection of tomato brown rugose fruit virus is influenced by infection at different growth stages and sampling from different plant parts

Detection of tomato brown rugose fruit virus is influenced by infection at different growth stages and sampling from different plant parts

Ensuring validation in diagnostic testing is fit for purpose: a view from the plant virology laboratory

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