Quality control in the histopathology laboratory: An overview with stress on the need for a structured national external quality assessment scheme

Iyengar, Jayaram N

doi:10.4103/0377-4929.44951

Cited by 18 publications

(30 citation statements)

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“…Artifacts can occur that influence the sample quality due to improper processing, sectioning, staining and mounting of the tissue. [8], [12] Second, tissue on the slide is never completely flat. Most slide scanners, such as the one used in this study, automatically determine multiple focus points before scanning.…”

Section: Discussionmentioning

confidence: 99%

SlideToolkit: An Assistive Toolset for the Histological Quantification of Whole Slide Images

et al. 2014

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The demand for accurate and reproducible phenotyping of a disease trait increases with the rising number of biobanks and genome wide association studies. Detailed analysis of histology is a powerful way of phenotyping human tissues. Nonetheless, purely visual assessment of histological slides is time-consuming and liable to sampling variation and optical illusions and thereby observer variation, and external validation may be cumbersome. Therefore, within our own biobank, computerized quantification of digitized histological slides is often preferred as a more precise and reproducible, and sometimes more sensitive approach. Relatively few free toolkits are, however, available for fully digitized microscopic slides, usually known as whole slides images. In order to comply with this need, we developed the slideToolkit as a fast method to handle large quantities of low contrast whole slides images using advanced cell detecting algorithms. The slideToolkit has been developed for modern personal computers and high-performance clusters (HPCs) and is available as an open-source project on github.com. We here illustrate the power of slideToolkit by a repeated measurement of 303 digital slides containing CD3 stained (DAB) abdominal aortic aneurysm tissue from a tissue biobank. Our workflow consists of four consecutive steps. In the first step (acquisition), whole slide images are collected and converted to TIFF files. In the second step (preparation), files are organized. The third step (tiles), creates multiple manageable tiles to count. In the fourth step (analysis), tissue is analyzed and results are stored in a data set. Using this method, two consecutive measurements of 303 slides showed an intraclass correlation of 0.99. In conclusion, slideToolkit provides a free, powerful and versatile collection of tools for automated feature analysis of whole slide images to create reproducible and meaningful phenotypic data sets.

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Section: Discussionmentioning

confidence: 99%

SlideToolkit: An Assistive Toolset for the Histological Quantification of Whole Slide Images

et al. 2014

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“…Assessment and implementation of quality control in histopathology is not easy as its output is wholly qualitative rather than quantitative. [2] An integrated coordination between technical and managerial activities along with highly skilled pathologist is essential for the continuous, unimpeded realization of high quality, error-free, efficient and effective laboratory operations. [3]…”

Section: Introductionmentioning

confidence: 99%

Quality and safety aspects in histopathology laboratory

Adyanthaya

Jose

2013

J Oral Maxillofac Pathol

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Histopathology is an art of analyzing and interpreting the shapes, sizes and architectural patterns of cells and tissues within a given specific clinical background and a science by which the image is placed in the context of knowledge of pathobiology, to arrive at an accurate diagnosis. To function effectively and safely, all the procedures and activities of histopathology laboratory should be evaluated and monitored accurately. In histopathology laboratory, the concept of quality control is applicable to pre-analytical, analytical and post-analytical activities. Ensuring safety of working personnel as well as environment is also highly important. Safety issues that may come up in a histopathology lab are primarily those related to potentially hazardous chemicals, biohazardous materials, accidents linked to the equipment and instrumentation employed and general risks from electrical and fire hazards. This article discusses quality management system which can ensure quality performance in histopathology laboratory. The hazards in pathology laboratories and practical safety measures aimed at controlling the dangers are also discussed with the objective of promoting safety consciousness and the practice of laboratory safety.

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“…The laboratories are associated with hazardous chemical that may pose a potential risk to workers, as well as, the environment. For this reason, many nations have now passed standards and regulations that are designed to improve working conditions and overcome the potential threats [5]. However, this standards and regulations vary from one country to another, but the underlying theme is universal.…”

Section: Introductionmentioning

confidence: 99%

The Role of Technical Quality Control in Histology Laboratories

Mohammedsaleh¹

2014

J Cytol Histol

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The importance of laboratories cannot be gainsaid as far as the health of any country is concerned. This is especially considering that laboratories have a bearing on the accuracy of research carried out on varied ailments, as well as the effectiveness of any remedial substance. Of course, there are numerous categories of laboratories with histology laboratories coming as some of the most fundamental. Histology laboratories come with a number of distinctive characteristics that can only be comprehended through comparing the laboratories with other areas of medical laboratories. Nevertheless, they also have to ensure the efficacy and integrity of their results. This underlines the importance of quality control in histology laboratories. Quality control underlines a system pertaining to routine technical activities that are aimed at developing and controlling the quality and accuracy of inventory while it is being developed. This system comes up with consistent and routine checks that identify and address omissions and errors, safeguard the integrity, completeness and correctness of data, while also recording all quality control activities.This Review Article examines the role of quality control in histology laboratories. It examines the varied aspects of quality management including risk identification, risk analysis and risk elimination. On the same note, it examines the convergence between quality management and quality assurance while paying close attention to the role of technical quality control in histology laboratories.

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Quality control in the histopathology laboratory: An overview with stress on the need for a structured national external quality assessment scheme

Cited by 18 publications

References 0 publications

SlideToolkit: An Assistive Toolset for the Histological Quantification of Whole Slide Images

SlideToolkit: An Assistive Toolset for the Histological Quantification of Whole Slide Images

Quality and safety aspects in histopathology laboratory

The Role of Technical Quality Control in Histology Laboratories

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