Quality Control Procedures for Stem Cell Lines

Stacey, Glyn; Auerbach, Jonathan M.

doi:10.1002/9780470167526.ch1

Cited by 12 publications

(4 citation statements)

References 52 publications

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“…Use of antibiotics should be avoided as it can mask the presence of microbial organisms, which then become detectable upon removal of the antibiotic, or worse still, initiate pathogenic events upon transplantation. Cells should be tested for the presence of bacteria, fungus and mycoplasma routinely in any cell culture lab (Stacey and Stacey, 2000), but adherence to strict testing protocols becomes even more important when working with cells that might be used for therapy, and microbial detection tests should be conducted after a minimum of 5 days' culture and preferably after two antibiotic-free passages to ensure that any suppressed microorganisms do not go undetected (Stacey and Auerbach, 2007). Microbial testing kits are therefore routinely used to test for microbial presence as a primary QC measure.…”

Section: Safetymentioning

confidence: 99%

Quality control in cell and tissue engineering

Wall

Davie

2013

Standardisation in Cell and Tissue Engineering

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Section: Safetymentioning

confidence: 99%

Quality control in cell and tissue engineering

Wall

Davie

2013

Standardisation in Cell and Tissue Engineering

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“…Mycoplasma iPSC banks should be tested for mycoplasma using US, European, Japanese pharmacopeia or otherwise nationally accredited pharmacopeia methods. Potential limitations of particular tests are sensitivity and test inhibition [8].…”

Section: Short Tandem Repeat Analysismentioning

confidence: 99%

Quality Control Guidelines for Clinical-Grade Human Induced Pluripotent Stem Cell Lines

Sullivan¹,

Stacey²,

et al. 2018

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Use of clinical-grade human induced pluripotent stem cell (iPSC) lines as a starting material for the generation of cellular therapeutics requires demonstration of comparability of lines derived from different individuals and in different facilities. This requires agreement on the critical quality attributes of such lines and the assays that should be used. Working from established recommendations and guidance from the International Stem Cell Banking Initiative for human embryonic stem cell banking, and concentrating on those issues more relevant to iPSCs, a series of consensus workshops has made initial recommendations on the minimum dataset required to consider an iPSC line of clinical grade, which are outlined in this report. Continued evolution of this field will likely lead to revision of these guidelines on a regular basis.

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“…Stacey (2007) 27 indicates that there are three fundamental characteristics to ensure the quality of work with cell lines: 1) purity, that is, that they are free of microorganisms; 2) identity, which refers to the cells’ being who they say they are; and 3) stability, indicating that the genotype and phenotype must remain unchanged during growth and in vitro passages. Other criteria highlighted by cell culture researchers are the verification of viability, karyotyping, confirmation of the species of origin, specific cell identification (genomic fingerprinting), cell markers, genetic expressions, pluripotency (in the case of stem cell tissues), as well as quality controls in culture.…”

Section: Introductionmentioning

confidence: 99%

“…Other criteria highlighted by cell culture researchers are the verification of viability, karyotyping, confirmation of the species of origin, specific cell identification (genomic fingerprinting), cell markers, genetic expressions, pluripotency (in the case of stem cell tissues), as well as quality controls in culture. 27 Cell morphology (phenotypic changes) and ploidy (genotypic changes) are also things to keep an eye on in cell lines to make sure they have the right biological properties. 28 …”

Section: Introductionmentioning

confidence: 99%

Establishment, authenticity, and characterization of cervical cancer cell lines

Martinez

Mendoza

Salazar

et al. 2022

Molecular & Cellular Oncology

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Cell lines have been considered excellent research models in many areas of biomedicine and, specifically, in the study of carcinogenesis. However, they cease to be effective models if their behavior changes. Although studies on the cross-contamination of cell lines originating from different tissues have been performed, little is known about cell lines derived from cervical neoplasia. We know that high-risk HPV (HR-HPV) is associated with the development of this type of cancer. This link between HPV infection and cancer was first established over 35 years ago when HPV16 DNA was found to be present in a large proportion of cervical cancer biopsies. The present review paper aims to report the status of the establishment, authenticity, and characterization of cervical cancer (CC) cell lines. This is a systematic review of articles on the establishment, authenticity, and characterization of CC cell lines, published from 1960 to date in the databases and in cell repository databases. 52 cell lines were identified in the literature. Only 25 cell lines were derived from cervical neoplasia, of which only 45.8% have a reported identity test (genomic fingerprint). Despite the increase in the establishment of cell lines of cervical neoplasia and the standards for the regulation of these study models, the criteria for their characterization continue to be diverse.

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Quality Control Procedures for Stem Cell Lines

Cited by 12 publications

References 52 publications

Quality control in cell and tissue engineering

Quality control in cell and tissue engineering

Quality Control Guidelines for Clinical-Grade Human Induced Pluripotent Stem Cell Lines

Establishment, authenticity, and characterization of cervical cancer cell lines

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