Quality evaluation of Yanghuo Sanqi tablet through a simultaneous determination of five major active Flavonoids and three main saponins by HPLC-DAD-ELSD

Abstract

Summary. Yanghuo Sanqi tablet (YST), combined prescription mainly derived from the leaves of Herba epimedii and the roots of Panax notoginseng, is a traditional Chinese medicine (TCM). Flavonoids (icarrin, epimedin A, epimedin B, epimedin C, and baohuoside I) and saponins (notoginsenoside R1, ginsenoside Rgl, and ginsenoside Rbl) are considered as the main bioactive compounds of YST. However, there is no report on quality control of TCMs by simultaneous determination of above-mentioned flavonoids and saponins so far. In this work, for the first time, a high-performance liquid chromatography−diode array detector−evaporative light scattering detector (HPLC−DAD−ELSD) method was developed to evaluate the quality of YST through a simultaneous determination of five major active flavonoids and three main saponins. Optimum separations were obtained with a Zorbax SB-C18 column by gradient elution with acetonitrile−water as the mobile phase. The drift tube temperature of ELSD was set at 105 °C, and the nebulizing gas flow rate was 2.5 L min −1 . The fully validated method was successfully applied to quantify the eight bioactive components in three lot products. This simple, low-cost, and reliable HPLC−DAD−ELSD method provided a new basis for assessing the quality of traditional Chinese medicinal compound preparations (TCMCPs) consisting of many bioactive components.

Simultaneous Determination of 5 Flavonoids and 7 Saponins for Quality Control of Traditional Chinese Medicine Preparation Xinnaoshutong Capsule Using HPLC-VWD-ELSD

“…In recent years, high performance liquid chromatography-diode array detector-evaporative light scattering detector (HPLC-DAD-ELSD) method was widely used in detecting the low ultraviolet absorption compounds, which is a simple, rapid, and accurate method and can reflect variations in the content of constituent components in herbal preparations and has been widely used for quality assessment of traditional Chinese herbal medicines and their preparations [ 25 – 27 ]. For the purposes of the quality of XC, an HPLC-VWD-ELSD (high performance liquid chromatography-variable wavelength detector-evaporative light scattering detector) was developed and validated to quantify some components.…”