Simultaneous Determination of 5 Flavonoids and 7 Saponins for Quality Control of Traditional Chinese Medicine Preparation Xinnaoshutong Capsule Using HPLC-VWD-ELSD

Abstract

Xinnaoshutong capsule (XC) is a traditional Chinese prescription derived from the ripe fruit of Tribulus terrestris L. (TT). Although XC has long been considered as an important herbal medicine, no analytical method of marker compounds for quality assessment is registered in the Chinese Pharmacopoeia. A simple analytical method of twelve marker components was developed and validated by HPLC-VWD-ELSD method. Chromatographic separation by HPLC was carried out on a Hedera ODS 2 column (4.6 × 250 mm, 5 μm) by gradient elution with acetonitrile-water (0.1% formic acid) as the mobile phase. Various extraction conditions were optimized to achieve twelve marker compounds with faster extraction and higher recovery. The analytical condition was then validated in terms of the linearity, accuracy and precision, repeatability, and stability. The twelve markers were successfully quantified in 30 batches of commercial samples. The developed HPLC-VWD-ELSD could be used as a rapid and reliable way in the assessment and quality control of XC and TT.

Diagnostic Fragment-Ion-Based for Rapid Identification of Chlorogenic Acids Derivatives in Inula cappa Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry

“…Based on the diversity and complexity of the chemical components of TCM, it is necessary to use advanced analytical techniques and methods to explain the pharmacological basis and mechanism of action of TCM because ultrahigh performance liquid chromatography-mass spectrometry (UHPLC-MS) with high sensitivity is to reduce sample complexity [ 2 ]. In addition, UHPLC-MS also has the advantages of good specificity, short analysis cycle, and good signal reproducibility [ 3 , 4 ]. UHPLC-HRMS has now been widely used in the analysis and identification of TCM components, especially most small molecules.…”

Green and Efficient Extraction of Polysaccharide and Ginsenoside from American Ginseng (Panax quinquefolius L.) by Deep Eutectic Solvent Extraction and Aqueous Two-Phase System

“…Linearity, precision, accuracy, and stability were used to evaluate the stability and feasibility of the method by the reported methods in the references [ 39 , 40 ]. Among them, the RSD value less than 3% was used as a standard to evaluate the stability of the method.…”

A review of HPLC technique covering its pharmaceutical, environmental, forensic, clinical and other applications

“…The proposed method could be readily utilized as a routine analysis and effective tool to evaluate the quality control of Traditional Chinese medicines. 41…”