Quality of fresh and cryopreserved semen and their influence on the rates of fertilization, hatching and quality of the larvae of Piaractus mesopotamicus

Galo, Juliana Minardi; Streit-Junior, D P; Oliveira, Cal; Povh, Jayme Aparecido; Fornari, Darci Carlos; Digmayer, Melanie; Ribeiro, Ricardo Pereira

doi:10.1590/1519-6984.182391

Cited by 18 publications

(20 citation statements)

References 11 publications

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“…It has been confirmed that the freezing process can cause injuries to both head and tail of sperm (Galo et al, 2019). CPAs' toxicity and temperature reduction are responsible for such sperm abnormalities.…”

Section: Discussionmentioning

confidence: 95%

Effects of Cryopreservation on Gene Expression and Post Thaw Sperm Quality of Pacific Abalone, Haliotis discus hannai

et al. 2021

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Pacific abalone, Haliotis discus hannai, is a high commercial seafood in South-East Asia. The aim of the present study was to determine effects of cryopreservation on gene expression and post thaw sperm quality of Pacific abalone. Two ions, Na+ (459.1 ± 3.1 mM) and Cl– (515.9 ± 1.1 mM), were predominant in the seminal plasma (pH: 6.8 ± 0.1; osmolarity: 1,126 ± 3 mOsmL–1). Cryopreservation reduced mRNA expression levels of protein kinase A (PKA-C) and heat shock proteins (HSP70 and HSP90) genes in sperm. Fluorescent technique was used to compare morphological defects, acrosome integrity (AI), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), and DNA integrity of sperm cryopreserved with five different cryopreservation solutions (8% Me2SO, 8% EG, 6% PG, 2% GLY, and 2% MeOH). Droplet in tail and coiled tail defects was not observed for sperm cryopreserved with 8% Me2SO or 2% GLY. Sperm cryopreserved with 8% Me2SO showed improved DNA integrity and lower cryodamage than sperm cryopreserved with other cryoprotectants. Sperm to egg ratio of 10,000:1 was found to be the most suitable ratio for in vitro fertilization among different ratios tested. The fertilization rate of sperm cryopreserved with 8% Me2SO was not significantly (p > 0.05) different from that of sperm cryopreserved with 2% GLY. DNA fragmentation showed strongly negative relationships with sperm quality parameters. Sperm cryopreserved with 8% Me2SO showed higher post thaw quality and mRNA expression of sperm motility associated gene than those cryopreserved with other cryoprotectants. The present research suggests to use 8% Me2SO for cryopreservation of Pacific abalone sperm as well as for hatchery production.

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Section: Discussionmentioning

confidence: 95%

Effects of Cryopreservation on Gene Expression and Post Thaw Sperm Quality of Pacific Abalone, Haliotis discus hannai

et al. 2021

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“…However, effects of cryopreservation could have been confounded with effects of intracytoplasmic sperm injection in their study. In fish, no effect of sperm cryopreservation on offspring performance could be found in some species [24][25][26][27][28][29], while significant effects on several developmental traits were reported in others [30][31][32][33]. In the latter case, effects of cryopreservation on potential indicators of offspring fitness were sometimes negative [30,31] and sometimes positive [32], and sometimes results were mixed [33].…”

Section: Introductionmentioning

confidence: 94%

Sperm cryopreservation reduces offspring growth

2019

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Sperm cryopreservation is routinely used in reproductive medicine, livestock production and wildlife management. Its effect on offspring performance is often assumed to be negligible, but this still remains to be confirmed in well-controlled within-subject experiments. We use a vertebrate model that allows us to experimentally separate parental and environmental effects to test whether sperm cryopreservation influences offspring phenotype under stress and non-stress conditions, and whether such effects are male-specific. Wild brown trout ( Salmo trutta ) were stripped for their gametes, and a portion of each male's milt was cryopreserved. Then, 960 eggs were simultaneously fertilized with either non-cryopreserved or frozen-thawed semen and raised singly in the presence or absence of a pathogen. We found no significant effects of cryopreservation on fertilization rates, and no effects on growth, survival nor pathogen resistance during the embryo stage. However, fertilization by cryopreserved sperm led to significantly reduced larval growth after hatching. Males varied in genetic quality as determined from offspring performance, but effects of cryopreservation on larval growth were not male-specific. We conclude that cryopreservation causes a reduction in offspring growth that is easily overlooked because it only manifests itself at later developmental stages, when many other factors affect growth and survival too.

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“…The removal of two of the studies (Galo et al, 2011; Streit et al, 2009) decreased the MD to −2.52% and −2.65% respectively. The removal of another study (Galo et al, 2019) increased the combined estimate, leaving the MD = −1.28%. For the percentage of cells with secondary abnormalities, the sensitivity analysis showed that the removal of three studies (Da Costa et al, 2019; Galo et al, 2011, 2019) increased the MD by 0.26% to 0.81, 0.46 and −0.32 respectively.…”

Section: Resultsmentioning

confidence: 95%

“…The removal of another study (Galo et al, 2019) increased the combined estimate, leaving the MD = −1.28%. For the percentage of cells with secondary abnormalities, the sensitivity analysis showed that the removal of three studies (Da Costa et al, 2019; Galo et al, 2011, 2019) increased the MD by 0.26% to 0.81, 0.46 and −0.32 respectively. On the other hand, the removal of the study by Streit et al (2009) decreased the combined estimate (MD = −0.15).…”

Section: Resultsmentioning

confidence: 95%

“…Gamete cryopreservation is considered an essential tool for the production of juveniles in laboratories (Galo et al, 2019); among reproductive biotechniques, semen storage was reported as being regularly used more than ten years ago by Mazur et al (2008). Cryopreservation involves adding a cryoprotectant solution to freezing and thawing semen samples.…”

Section: Introductionmentioning

confidence: 99%

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Morphological abnormalities in cryopreserved semen from South American neotropical fish: A meta‐analysis

Lassen

Canozzi

Costa

et al. 2021

Aquaculture Research

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A systematic review (SR) and meta‐analysis (MA) were performed to summarize the scientific evidence of the effects of cryopreservation of sperm from South American freshwater fish species on sperm morphology indicators. The search strategy was applied to four electronic databases, and specialists were contacted electronically. The main inclusion criteria involved studies on neotropical fish whose male gametes were submitted for cryopreservation. MA for random effects was performed for each indicator separately with the averages of the control and treated groups. A total of five publications, which comprise five studies and 15 trials, were included in the MA involving three different species. Heterogeneity was observed among the studies for all variables. In general, the percentage of normal cells did not change significantly after cryopreservation. Primary morphological abnormalities increased significantly at a level of 1.91% (95% confidence interval [CI]: −3.727–0.097; p = 0.021) after cryopreservation when compared with fresh semen. The percentage of secondary abnormalities did not show a significant change (p ≥ 0.05) between fresh semen and cryopreserved semen. Our results suggest a new classification for sperm abnormalities in South American neotropical fish based on the increase in primary abnormalities after cryopreservation.

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Quality of fresh and cryopreserved semen and their influence on the rates of fertilization, hatching and quality of the larvae of Piaractus mesopotamicus

Cited by 18 publications

References 11 publications

Effects of Cryopreservation on Gene Expression and Post Thaw Sperm Quality of Pacific Abalone, Haliotis discus hannai

Effects of Cryopreservation on Gene Expression and Post Thaw Sperm Quality of Pacific Abalone, Haliotis discus hannai

Sperm cryopreservation reduces offspring growth

Morphological abnormalities in cryopreserved semen from South American neotropical fish: A meta‐analysis

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