Quality of frozen-thawed testicular sperm and its preclinical use for intracytoplasmic sperm injection into in vitro-matured germinal-vesicle stage oocytes

Verheyen, Greta; Nagy, Zsolt; Joris, Hubert; Croo, Ilse De; Tournaye, Herman; Steirteghem, André Van

doi:10.1016/s0015-0282(97)81859-6

Cited by 71 publications

(40 citation statements)

References 20 publications

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“…Several studies have reported that the lower number and motility of frozen sperm might affect fertilization and pregnancy rates compared with fresh sperm [25][26][27]. However, no differences in fertilization, embryo cleavage, pregnancy, delivery, and spontaneous abortion rates were reported between fresh and frozen-thawed testicular sperm from men with OA and NOA patients [10,11,30,33]. On the other hand, Palermo et al [34] reported a lower fertilization rate for NOA patients (57 %) compared to OA patients (80 %); however, the clinical pregnancy rate was similar for both patient groups.…”

Section: Discussionmentioning

confidence: 99%

“…Previously Park et al [8] reported that acceptable fertilization and pregnancy rates were achieved using frozen testicular spermatozoa as opposed to fresh testicular spermatozoa in obstructive azoospermia (OA). Although the use of cryopreserved testicular sperm is not easy because of their low numbers and motility [3,9], there were no differences in fertilization and pregnancy rates for fresh and thawed testicular sperm from men with OA and non-obstructive azoospermia (NOA) [10,11]. Also, fertilization and pregnancies have been reported using thawed testicular sperm and testicular tissue [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of cryopreservation of embryos generated by intracytoplasmic sperm injection with spermatozoa from frozen testicular tissue

Park

Kim

Lim

et al. 2014

J Assist Reprod Genet

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Purpose To evaluate the viability of frozen embryos generated by intracytoplasmic sperm injection (ICSI) with frozen testicular spermatozoa. Methods A total of 68 fresh embryo transfer (ET) cycles and 85 subsequent frozen-thawed ET (FET) cycles were grouped according to the source of spermatozoa: fresh testicular spermatozoa (TESE) or frozen-thawed testicular spermatozoa (t-TESE). Results There were no significant differences in the age of female patients, number of oocytes, or fertilization rates in fresh ET cycles with TESE (TESE-fresh ET) versus t-TESE (t-TESE-fresh ET). The rate of embryo survival after thawing (95.7 % vs. 94.0 %) was similar in frozen ET cycles (FET) with TESE (TESE-FET) and with t-TESE (t-TESE-FET). While there were significant differences in the proportion of good quality embryos, no statistical differences were found in the pregnancy or clinical abortion rates between the two groups. Moreover, delivery rates were not significantly different. Conclusions Although the proportion of good quality embryos was affected by cryopreservation of testicular tissue, embryo survival rate was not. As well, subsequent pregnancy could be achieved successfully via t-TESE-FET cycles. Therefore, FET is not affected by the cryopreservation of testicular tissue, and avoids further oocyte retrieval and TESE procedures.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficacy of cryopreservation of embryos generated by intracytoplasmic sperm injection with spermatozoa from frozen testicular tissue

Park

Kim

Lim

et al. 2014

J Assist Reprod Genet

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“…More studies are needed to clarify the optimal physical and chemical methods for extended culture of testicular tissue. Although there are some reports indicating the superiority of fresh versus frozen-thawed testicular sperm from men with OA and NOA (27,30,31), many other studies found no difference in fertilization rates, PRs, and spontaneous abortion rates between fresh and frozen-thawed testicular sperm (24,32). Wu et al (15) observed a similar rate on day 3 high quality embryos and clinical PRs with fresh motile and frozen-thawed motile spermatozoa (54.2% vs. 54.1% and 59% vs. 55.5%, respectively) in ICSI cycles.…”

Section: Discussionmentioning

confidence: 99%

Outcome of intracytoplasmic sperm injection cycles with fresh testicular spermatozoa obtained on the day of or the day before oocyte collection and with cryopreserved testicular sperm in patients with azoospermia

Karacan

Alwaeely

Erkan

et al. 2013

Fertility and Sterility

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“…A period of 24 hours has been suggested as optimal for the development of motility in a sufficient number of testicular sperm to give choice in the treatment cycle [37]. However, the process of freeze-thawing has been found to significantly decrease the vitality of testicular sperm immediately post-thaw [95]. Ultrastructural cryoinjuries have also been observed in thawed testicular sperm in the form of rupturing of the plasma and acrosomal membranes [59].…”

Section: C R Y O I N J U R Y a N D T E S T I C U L A R S P E R M mentioning

confidence: 99%

“…Cryopreservation has been found to have a detrimental effect on the viability [66] and morphology of testicular and ejaculated sperm [95,60] due to the formation of intracellular ice, which causes the rupturing of plasma membranes [53]. Another group detected swelling and rupturing of the acrosomal and plasma membranes after freeze-thawing, also in testicular sperm [59].…”

Section: C R Y O I N J U R Y a N D T E S T I C U L A R S P E R M mentioning

confidence: 99%

Traitement de l’azoospermie obstructive par injection Intracytoplasmique d’un spermatozoïde

Lewis

2006

Androl.

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Intracytoplasmic sperm injection (ICSI) allows the treatment of virtually every type of male infertility. Unlike in vitro fertilization (IVF), its success does not depend on sperm concentration, motility or morphology and most of the physical barriers to fertilisation are by-passed. Since ICSl does not require strongly motile sperm, its use has now been expanded to incorporate immature sperm from the testes and epididymides. Successful fertilisation, pregnancies and healthy babies have all been reported. However, concerns about the safety of ICSl remain due to its short clinical history and the lack of testing on animal models.Male fertility potential for assisted reproduction by ICSl cannot be measured by conventional parameters. Sperm DNA integrity is increasingly recognised as a more useful indicator. Studies have shown that sperm with higher levels of DNA damage have lower fertilisation rates after IVF and ICSI. Sperm with DNA damage above a certain threshold are associated with a longer time to conceive in otherwise apparently fertile couples and a higher miscarriage rate. DNA damage has been shown to be associated with impaired embryo cleavage. Our group has shown that sperm DNA from testicular sperm is less fragmented than that from epididymal sperm and suggest its preferred use in ICSI.In addition to nuclear (n) DNA we also assessed the quality of mitochondrial (mt) DNA from testicular sperm from men with obstructive azoospermia undergoing ICSI. We observed that couples achieving a pregnancy had both less mtDNA deletions and less nDNA fragmentation. We found inverse relationships between pregnancy and sperm mtDNA deletion numbers, size and nDNA fragmentation. No relationships were observed with fertilisation rates. With this knowledge, we designed an algorithm for the prediction of pregnancy based on the quality of sperm nDNA and mtDNA.Each year 40,000 men have a vasectomy in the UK but every year 2500 request a reversal to begin a second family. For such men, vasectomy reversal has recently been replaced in part by testicular biopsy via fine-needle testicular sperm aspiration (TESA) or percutaneous epididymal sperm aspiration (PESA) performed at an outpatient clinic and subsequently used in ICSI. Since these were previously fertile men it has been assumed that they had 'fertile' sperm. However the assisted conception success rates of these mens partners has not been assessed until recently. We have shown a significant reduction in the clinical pregnancy rates in the partners of men who had had a vasectomy >10yrs previously. There is also evidence to suggest that spermatogenesis is significantly impaired in vasectomised men. Marked decreases in spermatocytes, spermatids and spermatozoa have been observed. We have found this to be associated with concomitant increases in apoptotic markers, such as Fas, FasL and Bax. The quality of the remaining sperm is also compromised. Sperm DNA from vasectomized men shows substantial damage which increases with time after surgery. This new use of ICSI will be d...

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Quality of frozen-thawed testicular sperm and its preclinical use for intracytoplasmic sperm injection into in vitro-matured germinal-vesicle stage oocytes

Cited by 71 publications

References 20 publications

Efficacy of cryopreservation of embryos generated by intracytoplasmic sperm injection with spermatozoa from frozen testicular tissue

Efficacy of cryopreservation of embryos generated by intracytoplasmic sperm injection with spermatozoa from frozen testicular tissue

Outcome of intracytoplasmic sperm injection cycles with fresh testicular spermatozoa obtained on the day of or the day before oocyte collection and with cryopreserved testicular sperm in patients with azoospermia

Traitement de l’azoospermie obstructive par injection Intracytoplasmique d’un spermatozoïde

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