Quality Related Safety Evaluation of a South African Traditional Formulation (PHELA®) as Novel Anti-Biofilm Candidate

Das, Bhaskar; Kar, Amit; Bhowmik, Rudranil; Karmakar, Sanmoy; Tripathy, Satyajit; Matsabisa, Motlalepula G.; Mukherjee, Pulok K.

doi:10.3390/molecules27041219

Cited by 4 publications

(4 citation statements)

References 51 publications

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“…The toxic effect of the extracts in HepG2 cells was performed via MTT cytotoxicity assay as described by Das et al, 2022. HepG2 cells were cultured and maintained in DMEM supplemented with 10% (v/v) FBS, L-glutamine (4 mM), penicillin (100 U/mL) and streptomycin (100 μg/ml). In each well of the 96-well microtiter plate, 100 µL of the media containing cell suspension (1×10 4 cells/mL) was seeded and incubated at 37 °C with 5% CO 2 for 24 h to allow the cells to attach to the bottom of the wells.…”

Section: In Vitro Cytotoxicity Assessmentmentioning

confidence: 99%

Section: Evaluation Of Anti-alzheimer’s Potential By An In-...mentioning

confidence: 99%

“…A fluorogenic assay was performed in black 96-well microplates following the method described by Das et al, 2022 . The test extract, 10 μM ketoconazole (CYP3A4), and 10 μM fluoxetine (CYP2D6) were dissolved in 100 mM Vivid CYP450 reaction buffer and diluted in 2-fold serial dilution.…”

Section: Evaluation Of Anti-alzheimer’s Potential By An In-...mentioning

confidence: 99%

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Evaluation of Mollugo oppositifolia Linn. as cholinesterase and β-secretase enzymes inhibitor

Das

Bhardwaj²,

Sharma³

et al. 2023

Front. Pharmacol.

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Mollugo oppositifolia Linn. is traditionally used in neurological complications. The study aimed to investigate in-vitro neuroprotective effect of the plant extracts through testing against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and β-secretase linked to Alzheimer’s disease (AD). To understand the safety aspects, the extracts were tested for CYP450 isozymes and human hepatocellular carcinoma cell (HepG2) inhibitory potential. The heavy metal contents were estimated using atomic absorption spectroscopy (AAS). Further, the antioxidant capacities as well as total phenolic content and total flavonoid content (TFC) were measured spectrophotometrically. UPLC-QTOF-MS/MS analysis was employed to identify phytometabolites present in the extract. The interactions of the ligands with the target proteins (AChE, BChE, and BACE-1) were studied using AutoDockTools 1.5.6. The results showed that M. oppositifolia extract has more selectivity towards BChE (IC50 = 278.23 ± 1.89 μg/ml) as compared to AChE (IC50 = 322.87 ± 2.05 μg/ml). The IC50 value against β-secretase was 173.93 μg/ml. The extract showed a CC50 value of 965.45 ± 3.07 μg/ml against HepG2 cells and the AAS analysis showed traces of lead 0.02 ± 0.001 which was found to be within the WHO prescribed limits. Moreover, the IC50 values against CYP3A4 (477.03 ± 2.01 μg/ml) and CYP2D6 (249.65 ± 2.46 μg/ml) isozymes justify the safety aspects of the extract. The in silico molecular docking analysis of the target enzymes showed that the compound menthoside was found to be the most stable and showed a good docking score among all the identified metabolites. Keeping in mind the multi-targeted drug approach, the present findings suggested that M. oppositifolia extract have anti-Alzheimer’s potential.

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Section: In Vitro Cytotoxicity Assessmentmentioning

confidence: 99%

Section: Evaluation Of Anti-alzheimer’s Potential By An In-...mentioning

confidence: 99%

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Evaluation of Mollugo oppositifolia Linn. as cholinesterase and β-secretase enzymes inhibitor

Das

Bhardwaj²,

Sharma³

et al. 2023

Front. Pharmacol.

Self Cite

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“…Instead of microbial cells, hydrated EPS makes up the majority of the biomass in the biofilms [48]. Microcolonies are created from the biofilm cluster by the continuous, dynamic spatial structuring of EPS molecules in the matrix [49]. It is also known that CUR prevents uropathogens from swimming and swarming [50].…”

Section: Mechanism Of Cur For Modulating Joint Inflammationmentioning

confidence: 99%

Role of Natural Products in Inflammatory Pharmacology: Harnessing Nature's Potential Through Drug Delivery for Therapeutic Intervention

Bhowmik,

Shaharyar,

Sarkar

et al. 2023

jchr

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Inflammatory disorders encompass a broad spectrum of conditions marked by abnormal immune responses and persistent inflammation, affecting healthcare and patient wellbeing. Seeking alternative, safe, and efficacious treatments, researchers are turning to natural products within the realm of inflammatory pharmacology. These products, sourced from plants, animals, and microorganisms, hold promise as therapeutic interventions. Natural products exhibit complex interactions with molecular targets and signaling pathways involved in the inflammatory cascade across various diseases. These interactions result in anti-inflammatory (AI) effects and provide potential therapeutic benefits. These advantages may be harnessed via diverse drug delivery methods used for administering these natural products. Plant-derived compounds like CUR (CUR), found in turmeric, exhibit robust AI effects by modulating inflammation-associated pathways. Resveratrol (RES), present in grapes and berries, mitigates inflammation and oxidative stress. Similarly, animal-derived products such as omega-3 fatty acids (OFG) and bee venom components, along with microbial-derived substances like probiotics and fungal metabolites, display notable AI properties. It presents an all-encompassing perspective on the role of natural products in inflammatory pharmacology, scrutinizing their molecular mechanisms, efficacy, and applicability through drug delivery approaches in diverse inflammatory disorders. By synthesizing the latest scientific strides, the article deepens our comprehension of the therapeutic potential of natural products in managing inflammatory conditions through various drug delivery approaches, thereby offering valuable insights for future research and clinical utilization.

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Advances in biofilm characterization: utilizing rheology and atomic force microscopy in foods and related fields

Wang,

Xue,

Zhu

et al. 2024

Adv Compos Hybrid Mater

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Quality Related Safety Evaluation of a South African Traditional Formulation (PHELA®) as Novel Anti-Biofilm Candidate

Cited by 4 publications

References 51 publications

Evaluation of Mollugo oppositifolia Linn. as cholinesterase and β-secretase enzymes inhibitor

Evaluation of Mollugo oppositifolia Linn. as cholinesterase and β-secretase enzymes inhibitor

Role of Natural Products in Inflammatory Pharmacology: Harnessing Nature's Potential Through Drug Delivery for Therapeutic Intervention

Advances in biofilm characterization: utilizing rheology and atomic force microscopy in foods and related fields

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