Quantification by affinity perfusion chromatography of phosphorylated BRCAl and BRCA2 proteins from tumor cells after lycopene treatment

Chalabi, Nasséra; Maurizis, Jean‐Claude; Corre, Ludovic Le; Delort, Laëtitia; Bignon, Yves‐Jean; Bernard‐Gallon, Dominique

doi:10.1016/j.jchromb.2005.05.006

Cited by 9 publications

(3 citation statements)

References 35 publications

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“…These results were due to posttranscriptional and posttranslational modifications. Although no variation was observed for BRCA1 and BRCA2 proteins upon exposure to lycopene, increased levels of phosphorylated BRCA1 and BRCA2 proteins were observed (Chalabi et al 2005). It would therefore be of interest to assess the phosphorylation status of RARβ protein, along with its acetylation or ubiquitination levels.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Expression of RARα, RARβ, and Cx43 in Breast Tumor Cell Lines After Treatment With Lycopene and Correlation With RT-QPCR

Chalabi

Delort

Satih

et al. 2007

J Histochem Cytochem.

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Lycopene, the major carotenoid found in tomatoes, is a potent antioxidant associated with the prevention of degenerative diseases such as breast cancer. This effect could be due to the interaction between lycopene and retinoic acid receptors as well as the stimulation of gap junction communication and synthesis of connexin 43. The expression of the RARalpha, RARbeta, and Cx43 proteins was analyzed using immunohistochemistry in two breast cancer cell lines, MCF-7 and MDA-MB-231, and in a fibrocystic dystrophy cell line, MCF-10a, after a 48-hr exposure to 10 microM lycopene. A real-time quantitative PCR analysis was then performed to measure mRNA expression. RARalpha and Cx43 expression were increased at both mRNA and protein levels in two breast cell lines.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical Expression of RARα, RARβ, and Cx43 in Breast Tumor Cell Lines After Treatment With Lycopene and Correlation With RT-QPCR

Chalabi

Delort

Satih

et al. 2007

J Histochem Cytochem.

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“…Treatment with the lycopene increased the mRNA expression levels of BRCA1 (55). In addition, lycopene increases phosphorylation of BRCA1 in breast cancer cells (56). Treatment with phytoestrogens result in higher expression levels of BRCA1 by reversing DNA hypermethylation (57), and individuals with phytoestrogen-rich diets possessed increased expression levels of BRCA1 mRNA (58).…”

Section: Certain Dietary Compounds Exhibit Neuroprotective Effects Bymentioning

confidence: 99%

Special bioactive compounds and functional foods may exhibit neuroprotective effects in patients with dementia (Review)

et al. 2020

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Dementia is a failure of cognitive ability characterized by severe neurodegeneration in select neural systems, and Alzheimer's disease (AD) is the most common type of neurodegenerative disease. Although numerous studies have provided insights into the pathogenesis of AD, the underlying signaling and molecular pathways mediating the progressive decline of cognitive function remain poorly understood. Recent progress in molecular biology has provided an improved understanding of the importance of molecular pathogenesis of AD, and has proposed an association between DNA repair mechanisms and AD. In particular, the fundamental roles of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) and breast cancer gene 1 (BRCA1) tumor suppressors have been shown to regulate the pathogenesis of neurodegeneration. Consequently, onset of neurodegenerative diseases may be deferred with the use of dietary neuroprotective agents which alter the signaling mediated by the aforementioned tumor suppressors. In a healthy neuron, homeostasis of key intracellular molecules is of great importance, and preventing neuronal apoptosis is one of the primary goals of treatments designed for dementia-associated diseases. In the present review, progress into the understanding of dietary regulation for preventing or limiting development of dementia is discussed with a focus on the modulatory roles of PTEN and BRCA1 signaling.

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“…Treatment with lycopene increases BRCA1 gene expression in breast cancer cell line MCF-7 and HBL-100 [ 57 ]. Furthermore, treatment with lycopene increases phospho-BRCA1 in MCF-7 cells [ 58 ]. Manganese-induced perturbation in the antioxidant system with increase of acetylcholinesterase as demonstrated by AD is prevented by lycopene treatment [ 59 ], suggesting potential protective effects of lycopene against AD.…”

Section: Some Diets Involved In Tumor Suppressor Expression May Comentioning

confidence: 99%

BRCA1 and p53 Tumor Suppressor Molecules in Alzheimer’s Disease

Nakanishi

Minami

Kitagishi

et al. 2015

IJMS

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Tumor suppressor molecules play a pivotal role in regulating DNA repair, cell proliferation, and cell death, which are also important processes in the pathogenesis of Alzheimer’s disease. Alzheimer’s disease is the most common neurodegenerative disorder, however, the precise molecular events that control the death of neuronal cells are unclear. Recently, a fundamental role for tumor suppressor molecules in regulating neurons in Alzheimer’s disease was highlighted. Generally, onset of neurodegenerative diseases including Alzheimer’s disease may be delayed with use of dietary neuro-protective agents against oxidative stresses. Studies suggest that dietary antioxidants are also beneficial for brain health in reducing disease-risk and in slowing down disease-progression. We summarize research advances in dietary regulation for the treatment of Alzheimer’s disease with a focus on its modulatory roles in BRCA1 and p53 tumor suppressor expression, in support of further therapeutic research in this field.

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Quantification by affinity perfusion chromatography of phosphorylated BRCAl and BRCA2 proteins from tumor cells after lycopene treatment

Cited by 9 publications

References 35 publications

Immunohistochemical Expression of RARα, RARβ, and Cx43 in Breast Tumor Cell Lines After Treatment With Lycopene and Correlation With RT-QPCR

Immunohistochemical Expression of RARα, RARβ, and Cx43 in Breast Tumor Cell Lines After Treatment With Lycopene and Correlation With RT-QPCR

Special bioactive compounds and functional foods may exhibit neuroprotective effects in patients with dementia (Review)

BRCA1 and p53 Tumor Suppressor Molecules in Alzheimer’s Disease

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