Quantification in bioimaging by LA-ICPMS - Evaluation of isotope dilution and standard addition enabled by micro-droplets

A systematic study of high resolution multielemental quantitative bioimaging of animal tissue using LA-ICP-TOFMS

“…22 The developments in the ICP-TOFMS provide a solid analytical platform for all areas of solid-state analysis and, in particular, for bioimaging where fast multielemental detection is a paramount. 10,18,[23][24][25][26][27][28][29][30] Quantication strategies for silicate materials in LA-ICP-MS typically include external calibration using one or more certi-ed reference materials (CRMs) with a silicate glass matrix and with the intensity corrected by the intensity of an internal standard (IS). The IS is typically one of the major components of the sample and it may be determined by an independent technique.…”

“…Both materials can be spiked with the elements of interest at different concentration levels, providing multi-point calibration curves, and can be frozen and sliced to the same thickness as the material to be imaged, to provide direct comparison. [35][36][37][38] Over the past few years, calibration with spiked gelatin presented as either cryosections [39][40][41][42] or droplets, deposited manually, 43,44 in microarrays 45 or using a microspotter 27,28 or a bioprinter, 30 has largely replaced matrix-matched calibration with spiked homogenised tissue in LA-ICP-MS bioimaging. Despite the wide use of gelatin standards, quantication using gelatin cryosections has not yet been studied systematically and applied to single-shot high-resolution imaging.…”

“…22 The developments in the ICP-TOFMS provide a solid analytical platform for all areas of solid-state analysis and, in particular, for bioimaging where fast multielemental detection is a paramount. 10,18,23–30…”

“…Over the past few years, calibration with spiked gelatin presented as either cryosections 39–42 or droplets, deposited manually, 43,44 in microarrays 45 or using a microspotter 27,28 or a bioprinter, 30 has largely replaced matrix-matched calibration with spiked homogenised tissue in LA-ICP-MS bioimaging. Despite the wide use of gelatin standards, quantification using gelatin cryosections has not yet been studied systematically and applied to single-shot high-resolution imaging.…”

The entire periodic table in one droplet - semiquantiative analysis for high-speed mapping applications using LA-ICP-TOFMS

“…The latter ones were further developed as micro-droplet standards 19,20 to increase throughput in total ablation approaches by decreasing their size through automated and precise deposition by a micro-spotting device. 20,21 Moreover, aspiration of a standard solution (in-cell or in-torch) during laser sampling and applications of a polymer-based thin film standard on/under a biological sample combined with total consumption (i.e. ablation of the entire depth) of the assembly have been reported.…”