Quantification of 2-NBDG, a probe for glucose uptake, in GLUT1 overexpression in HEK293T cells by LC–MS/MS

Sun, Yanhong; Hu, Minwan; Wang, Fenghe; Tan, Huixin; Hu, Jiahuan; Wang, Xinbo; Wang, Baolian; Li, Yan

doi:10.1016/j.ab.2021.114357

Cited by 6 publications

(2 citation statements)

References 30 publications

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“…Hence, the HepG2 cell line has extensive applications in cell toxicity experiments. On the other hand, the HEK293T cell line has been utilized in research related to mitochondria and antiviral drugs, cell apoptosis, and glucose transport proteins [41,42]. Considering these factors, both of these cell lines were chosen for our experimental work.…”

Section: Discussionmentioning

confidence: 99%

Anisomeles indica Extracts and Their Constituents Suppress the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro

Chen,

Jiang,

Deng

et al. 2023

IJMS

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Coronavirus disease 2019 (COVID-19), stemming from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has had a profound global impact. This highly contagious pneumonia remains a significant ongoing threat. Uncertainties persist about the virus’s effects on human health, underscoring the need for treatments and prevention. Current research highlights angiotensin-converting enzyme 2 (ACE2) and transmembrane protease serine 2 (TMPRSS2) as key targets against SARS-CoV-2. The virus relies on ACE2 to enter cells and TMPRSS2 to activate its spike protein. Inhibiting ACE2 and TMPRSS2 expression can help prevent and treat SARS-CoV-2 infections. Anisomeles indica (L.) Kuntze, a medicinal plant in traditional Chinese medicine, shows various promising pharmacological properties. In this study, ethanolic extracts of A. indica were examined both in vivo (250 and 500 μM) and in vitro (500 μM). Through Western blotting analysis, a significant reduction in the expression levels of ACE2 and TMPRSS2 proteins was observed in HepG2 (human hepatocellular carcinoma) cells and HEK 293T (human embryonic kidney) cell lines without inducing cellular damage. The principal constituents of A. indica, namely, ovatodiolide (5 and 10 μM), anisomlic acid (5 and 10 μM), and apigenin (12.5 and 25 μM), were also found to produce the same effect. Furthermore, immunohistochemical analysis of mouse liver, kidney, and lung tissues demonstrated a decrease in ACE2 and TMPRSS2 protein expression levels. Consequently, this article suggests that A. indica and its constituents have the potential to reduce ACE2 and TMPRSS2 protein expression levels, thus aiding in the prevention of SARS-CoV-2 infections.

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Section: Discussionmentioning

confidence: 99%

Anisomeles indica Extracts and Their Constituents Suppress the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro

Chen,

Jiang,

Deng

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…It was found that 93% of the compounds could be tested for toxicity by HepG2 because of the low metabolic capacity of HepG2 [36] . Human embryonic kidney 293T cells have been used in experiments to study apoptosis, glucose transporters, mitochondria, and antiviral drugs [38] , [39] , [40] , [41] . Therefore, we chose these two cell lines for the subsequent experiments.…”

Section: Discussionmentioning

confidence: 99%

Study on the potential of Sanghuangporus sanghuang and its components as COVID-19 spike protein receptor binding domain inhibitors

Chien

Deng

Jiang

et al. 2022

Biomedicine & Pharmacotherapy

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Strain differences in the drug transport capacity of intestinal glucose transporters in Sprague–Dawley versus Wistar rats, C57BL/6J versus Kunming mice

Huang

Lin

Chen

et al. 2023

International Journal of Pharmaceutics

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Quantification of 2-NBDG, a probe for glucose uptake, in GLUT1 overexpression in HEK293T cells by LC–MS/MS

Cited by 6 publications

References 30 publications

Anisomeles indica Extracts and Their Constituents Suppress the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro

Anisomeles indica Extracts and Their Constituents Suppress the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro

Study on the potential of Sanghuangporus sanghuang and its components as COVID-19 spike protein receptor binding domain inhibitors

Strain differences in the drug transport capacity of intestinal glucose transporters in Sprague–Dawley versus Wistar rats, C57BL/6J versus Kunming mice

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