Quantification of arginine and its mono- and dimethylated analogs NMMA, ADMA and SDMA in biological fluids by LC–MS/MS: Is LC superfluous?

Martens‐Lobenhoffer, Jens; Schwedhelm, Edzard; Tsikas, Dimitrios

doi:10.1016/j.jchromb.2009.07.003

Cited by 23 publications

(28 citation statements)

References 27 publications

(78 reference statements)

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“…This conclusion is greatly supported by the results and chromatograms reported by this group [117] (see also Ref. [3]). Indeed, Sicilia et al [117] obtained completely different results from the LC-MS/MS quantification of 8-iso-PGF 2␣ in rat urine after direct injection of the diluted urine sample, i.e., without any sample treatment, as compared to the use of IAC.…”

Section: Review Of Applications In the Decade 2000-2009supporting

confidence: 67%

“…There are examples in the literature that minimization of sample preparation, for instance by simple matrix dilution, or renunciation of any sample preparation step or even the main LC separation step in quantitative analyses will definitely fail for numerous analytes [3]. Therefore, reliable quantitative analysis by LC-MS/MS regularly requires performance of adequate sample preparation procedures prior to quantification in addition to the LC step and the use of stable-isotope labelled analogs as internal standards (IS) at a proper concentration [4] (see Scheme 1).…”

mentioning

confidence: 99%

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Quantitative analysis of biomarkers, drugs and toxins in biological samples by immunoaffinity chromatography coupled to mass spectrometry or tandem mass spectrometry: A focused review of recent applications

Tsikas

2010

Journal of Chromatography B

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Section: Review Of Applications In the Decade 2000-2009supporting

confidence: 67%

mentioning

confidence: 99%

Quantitative analysis of biomarkers, drugs and toxins in biological samples by immunoaffinity chromatography coupled to mass spectrometry or tandem mass spectrometry: A focused review of recent applications

Tsikas

2010

Journal of Chromatography B

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“…The values were calculated as the mean ± SD. [26], and some pervious methods selected different product ion [18,19]. In our MS system, the product ions were as follows: m/z 40.1 for ADMA and m/z 129.1 for SDMA.…”

Section: Statistical Analysesmentioning

confidence: 99%

Determination of Asymmetric Dimethylarginine and Symmetric Dimethylarginine in Biological Samples of Mice Using LC/MS/MS

Saigusa¹,

Takahashi²,

Kanemitsu³

et al. 2011

AJAC

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Herein, we present a novel method of asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) determination within biological samples using protein precipitation and LC/MS/MS. Chromatographic separation of ADMA and SDMA was successfully performed using a silica column with optimized elution, or mobile phase, of 10 mM ammonium acetate buffer H 2 O/methanol/acetonitrile (20/35/45, v/v) at pH 4. The calibration ranges were 0.50 -50.0 µg·mL -1 , and good linearities were obtained for all compounds ( r > 0.99). The intra-and inter-assay accuracies with recoveries and precisions at three concentration levels (i.e. 1.00, 5.00 and 25.0 µg·mL -1 ) were better than 86.9% and 7.36%, respectively. The analytical performance of the method was evaluated by determination of compounds in plasma, urine and tissues from male BALBc/J mice. For the first time, we were able to characterize the distribution of ADMA, SDMA and AD-MA/SDMA in plasma, urine, brain, heart, kidneys, liver, lungs, pancreas and spleen. Additionally, we demonstrated that the ADMA/SDMA ratio in the brain was approximately 10-fold lower than all the other biological samples. Only 10 µL of plasma, 1 µL of urine and about 25 mg of tissues were required. These results suggest that the developed methodology was useful in ADMA and SDMA determination within biological samples.

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“…However, as comments Martens-Lobenhoffer [49], this is not suitable because this bring to loss of selectivity and accuracy in the quantitative determination and it is necessary to minimize matrix-associated effects.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantitative determination of NG,NG-dimethyl-l-arginine or asymmetric dimethylarginine and related pathway's metabolites in biological fluids by ultrahigh-performance liquid chromatography/electrospray ionization-tandem mass spectrometry

Gangi

Chiandetti

Gucciardi

et al. 2010

Analytica Chimica Acta

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Quantification of arginine and its mono- and dimethylated analogs NMMA, ADMA and SDMA in biological fluids by LC–MS/MS: Is LC superfluous?

Cited by 23 publications

References 27 publications

Quantitative analysis of biomarkers, drugs and toxins in biological samples by immunoaffinity chromatography coupled to mass spectrometry or tandem mass spectrometry: A focused review of recent applications

Quantitative analysis of biomarkers, drugs and toxins in biological samples by immunoaffinity chromatography coupled to mass spectrometry or tandem mass spectrometry: A focused review of recent applications

Determination of Asymmetric Dimethylarginine and Symmetric Dimethylarginine in Biological Samples of Mice Using LC/MS/MS

Simultaneous quantitative determination of NG,NG-dimethyl-l-arginine or asymmetric dimethylarginine and related pathway's metabolites in biological fluids by ultrahigh-performance liquid chromatography/electrospray ionization-tandem mass spectrometry

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