Quantification of betamethasone in human plasma by liquid chromatography–tandem mass spectrometry using atmospheric pressure photoionization in negative mode

Pereira, Alberto dos Santos; Oliveira, Lina S.O.B.; Mendes, Gustavo Duarte; Gabbai, Jorge J.; Nucci, Gilberto De

doi:10.1016/j.jchromb.2005.09.018

Cited by 23 publications

(18 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Liquid-liquid extraction is a more selective procedure with the possibility to concentrate the analyte by evaporation of the extract. It has been successfully applied for several LC-MS/MS assays for corticosteroids in plasma [7][8][9]. The hexane-diethyl ether mixture was chosen as the extraction solvent mixture because of the possibility to optimize the ratio of both solvents.…”

Section: Methods Developmentmentioning

confidence: 99%

Liquid chromatography–tandem mass spectrometric assay for clobetasol propionate in human serum from patients with atopic dermatitis

Sparidans¹,

Velsen²,

Roos³

et al. 2010

Journal of Chromatography B

View full text Add to dashboard Cite

Section: Methods Developmentmentioning

confidence: 99%

Liquid chromatography–tandem mass spectrometric assay for clobetasol propionate in human serum from patients with atopic dermatitis

Sparidans¹,

Velsen²,

Roos³

et al. 2010

Journal of Chromatography B

View full text Add to dashboard Cite

“…This ionization mode has been shown to be a powerful technique in analyzing different drugs in biological matrixes, including polar compounds such as chloramphenicol [16]. This procedure opens a new window to compounds that can be analyzed by LC-MS/MS, as for example in the present case of isosorbide 5-mononitrate.…”

Section: Methods Developmentmentioning

confidence: 99%

Quantification of isosorbide 5-mononitrate in human plasma by liquid chromatography–tandem mass spectrometry using atmospheric pressure photoionization

Silva

Oliveira²,

Mendes³

et al. 2006

Journal of Chromatography B

Self Cite

View full text Add to dashboard Cite

“…As the free alcohol, betamethasone finds widespread clinical applications related to its anti-inflammatory and immunosuppressant activity [3]. BE is frequently employed for allergic disease treatment and it is an active component of drops, injections, and topical creams.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, there is only one publication describing simultaneous determination of both compounds, but CL was used as internal standard [3]. This paper describes a specific, simple, and fast sequential injection chromatography method for the simultaneous determination of BE and CL in pharmaceutical ophthalmic solutions.…”

Section: Introductionmentioning

confidence: 99%

Simple determination of betamethasone and chloramphenicol in a pharmaceutical preparation using a short monolithic column coupled to a sequential injection system

Šatı́nský¹,

Chocholouš

Salabová

et al. 2006

J of Separation Science

View full text Add to dashboard Cite

This contribution describes the use of a new separation method based on a reversed-phase sequential injection chromatography (SIC) technique for simultaneous determination of chloramphenicol and betamethasone in pharmaceutical eye drops. A short monolithic column coupled with a sequential injection analysis (SIA) system enabled separation of two compounds in one step. A Chromolith Flash RP-18e, 25 x 4.6 mm column with a 5 mm precolumn (Merck, Germany) and a FIA1ab 3000 system (USA) with a 6-port selection valve and 5 mL syringe were used for sequential injection chromatographic separations in this study. The mobile phase used was acetonitrile-water (30:80, v/v), flow rate 0.48 mL/min; UV detection was at two wavelengths, i.e., 241 and 278 nm (absorption maxima of betamethasone and chloramphenicol, respectively). The basic validation parameters showed good results: linearity of determination for both compounds including internal standard (propylparaben) >0.999; repeatability of determination (RSD) in the range 0.8-1.7% at two different concentration levels, and detection limits in the range 0.5-1.0 mg/mL. The chromatographic resolution between compound peaks was greater than 2.1 and the analysis time was less than 8 min under optimal conditions. The developed sequential injection chromatography method was compared with the HPLC method and was found to be applicable for routine analysis of active compounds in pharmaceutical preparations.

show abstract

Quantification of betamethasone in human plasma by liquid chromatography–tandem mass spectrometry using atmospheric pressure photoionization in negative mode

Cited by 23 publications

References 26 publications

Liquid chromatography–tandem mass spectrometric assay for clobetasol propionate in human serum from patients with atopic dermatitis

Liquid chromatography–tandem mass spectrometric assay for clobetasol propionate in human serum from patients with atopic dermatitis

Quantification of isosorbide 5-mononitrate in human plasma by liquid chromatography–tandem mass spectrometry using atmospheric pressure photoionization

Simple determination of betamethasone and chloramphenicol in a pharmaceutical preparation using a short monolithic column coupled to a sequential injection system

Contact Info

Product

Resources

About