Quantification of biofilm production on polystyrene by Listeria, Escherichia coli and Staphylococcus aureus isolated from a poultry slaughterhouse

Rodrigues, Laura Beatriz; Santos, Luciana Ruschel dos; Tagliari, Vinícius Zancanaro; Rizzo, Natalie Nadin; Trenhago, Graciela; Oliveira, Amauri Picollo de; Goetz, Franciane; Nascimento, Vladimir Pinheiro do

doi:10.1590/s1517-83822010000400029

Cited by 58 publications

(36 citation statements)

References 3 publications

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“…Bacterial study. Five collection strains and widely known biofilm producers, that is, S. aureus 15981 (77-80), S. epidermidis ATCC 35984 (81)(82)(83), E. coli ATCC 25922 (84)(85)(86), P. aeruginosa ATCC 27853 (87)(88)(89), and S. maltophilia ATCC 13637 (90,91), were used. All the strains were kept frozen at Ϫ80°C until the experiments were performed.…”

Section: Methodsmentioning

confidence: 99%

Microbiological and Cellular Evaluation of a Fluorine-Phosphorus-Doped Titanium Alloy, a Novel Antibacterial and Osteostimulatory Biomaterial with Potential Applications in Orthopedic Surgery

Aguilera-Correa

Mediero

Conesa-Buendía

et al. 2019

Appl Environ Microbiol

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Joint prosthesis failure is mainly related to aseptic loosening and prosthetic joint infections, both of which are associated with high morbidity and substantial costs for patients and health systems. The development of a biomaterial that is capable of stimulating bone growth while minimizing bacterial adhesion would reduce the incidence of prosthetic failure. We report antibacterial and osteostimulatory effects in a novel fluorine-phosphorus (F-P)-doped TiO2 oxide film grown on Ti-6Al-4V alloy with a nanostructure of bottle-shaped nanotubes (bNT) using five bacterial species (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia) and MCT3T3-E1 osteoblastic cells. The interaction between the bacteria and bNT Ti-6Al-4V was complex, as the adhesion of four bacterial species decreased (two staphylococcus species, E. coli, and S. maltophilia), and the viability of staphylococci and S. maltophilia also decreased because of the aluminum (Al) released by bNT Ti-6Al-4V. This released Al can be recruited by the bacteria through siderophores and was retained only by the Gram-negative bacteria tested. P. aeruginosa showed higher adhesion on bNT Ti-6Al-4V than on chemically polished (CP) samples of Ti-6Al-4V alloy and an ability to mobilize Al from bNT Ti-6Al-4V. The cell adhesion and proliferation of MCT3T3-E1 osteoblastic cells significantly increased at 48 and 168 h, as did the matrix mineralization of these cells and the gene expression levels of three of the most important markers related to bone differentiation. According to our results, the bNT Ti-6Al-4V alloy could have clinical application, preventing infection and stimulating bone growth and thus preventing the two main causes of joint prosthesis failure. IMPORTANCE This work evaluates F-P-doped bNT Ti-6Al-4V from microbiological and cellular approaches. The bacterial results highlight that the antibacterial ability of bNT Ti-6Al-4V is the result of a combination of antiadhesive and bactericidal effects exerted by Al released from the alloy. The cell results highlight that F-P bNT Ti-6Al-4V alloy increases osseointegration due to modification of the chemical composition of the alloy resulting from P incorporation and not due to the nanostructure, as reported previously. A key finding was the detection of Al release from inside the bNT Ti-6Al-4V nanostructures, a result of the nanostructure growth during the anodizing process that is in part responsible for its bactericidal effect.

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Section: Methodsmentioning

confidence: 99%

Microbiological and Cellular Evaluation of a Fluorine-Phosphorus-Doped Titanium Alloy, a Novel Antibacterial and Osteostimulatory Biomaterial with Potential Applications in Orthopedic Surgery

Aguilera-Correa

Mediero

Conesa-Buendía

et al. 2019

Appl Environ Microbiol

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“…These isolates were previously identified by Martins et al (2013) and Riboldi et al (2009 Quantitative determination of biofilm was determined by crystal violet assay (CV) in accordance with Stepanović et al (2007) with some modifications. This methodology was chosen, for being the reference protocol to detection of biofilm formed by isolated food pathogens (Rodrigues et al 2010, Marinho et al 2013, Pinto et al 2015. In summary, three to five colonies were suspended in 4 ml of 0.85% (w/v) NaCl solution and compared with 0. glucose was added to each well and the microplate was incubated at 37ºC for 24 h. After incubation the plate was washed with 0.85% (w/v) NaCl solution for three times to remove non-adherent cells, 200 µl of 100% methyl alcohol was added for 20 min to fix the adhered cells.…”

Section: Bacterial Strainsmentioning

confidence: 99%

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Costa

Rossatto

Medeiros

et al. 2018

An. Acad. Bras. Ciênc.

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The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocinlike substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods. The BLS P34 showed inhibitory effect against all planktonic cells of E. faecalis. The inhibition of biofilm formation and the eradication of pre-formed biofilm were evaluated with the crystal violet assay and with the reduction of 3-bromide [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium. The BLS P34 promoted a reduction of percentage of adhered microbial cells on the surface, not being able to perform the complete elimination of biofilm formation. The metabolic activity of S. aureus biofilms decreased considerably between 41-95%. However, E. faecalis cells showed up metabolically stimulated. The BLS P34 has the potential antibiofilm for the species S. aureus. Studies suggest more detailed approaches to a better understanding of the interactions between the antimicrobial and bacterial cells within the biofilm structure.

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“…Several studies have reported high biofilm formation capacity of L. monocytogenes on polystyrene material (Rodrigues et al, 2010;Kadam et al, 2013) and thus reveal that the material is propitious to colonization by L. monocytogenes biofilms.…”

Section: Resultsmentioning

confidence: 99%

Resistance to disinfectants and antibiotics of Pseudomonas spp. and Listeria spp. biofilms on polystyrene and stainless steel

Demoliner¹,

Souza²,

Pacheco³

et al. 2015

Afr. J. Microbiol. Res.

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The capacity of Pseudomonas spp. and Listeria spp. isolates in forming polystyrene and stainless steel biofilms was assessed and their resistance to disinfectants and antibiotics agents was verified. Isolates originated from chicken and buffalo meat cuts in abattoirs and retail outlets in the southern region of the state of Rio Grande do Sul, Brazil. Isolates which formed stainless steel biofilm were tested with regard to the activities of the disinfectant agents organic chlorine and ammonium quaternary. Isolates of L. monocytogenes formed polystyrene and stainless steel biofilm. Further, 32 and 72% of Pseudomonas spp. isolates respectively formed polystyrene and stainless steel biofilm. The disinfectant agent ammonium quaternary was more efficient than organic chlorine in the decrease of biofilms on stainless steel surfaces for Listeria isolates. Multi-resistance to antibiotics was high for Listeria spp. (94.7%) and Pseudomonas spp (84%). From these results, isolates from chicken and buffalo meat cuts were developers of biofilm on polystyrene and stainless steel, and resistants' to antibiotics, putting at risk consumers´ health.

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Quantification of biofilm production on polystyrene by Listeria, Escherichia coli and Staphylococcus aureus isolated from a poultry slaughterhouse

Cited by 58 publications

References 3 publications

Microbiological and Cellular Evaluation of a Fluorine-Phosphorus-Doped Titanium Alloy, a Novel Antibacterial and Osteostimulatory Biomaterial with Potential Applications in Orthopedic Surgery

Microbiological and Cellular Evaluation of a Fluorine-Phosphorus-Doped Titanium Alloy, a Novel Antibacterial and Osteostimulatory Biomaterial with Potential Applications in Orthopedic Surgery

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Resistance to disinfectants and antibiotics of Pseudomonas spp. and Listeria spp. biofilms on polystyrene and stainless steel

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