Quantification of biotin in feed, food, tablets, and premixes using HPLC–MS/MS

Höller, Ulrich; Wachter, Frédérique; Wehrli, Christof; Fizet, Christian

doi:10.1016/j.jchromb.2005.11.021

Cited by 51 publications

(28 citation statements)

References 11 publications

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“…Due to the extensive phosphorylated and protein-bound vitamins in natural foods, quantitative analysis of the entire array of B-group vitamins at endogenous levels requires vigorous sample extraction to release the free vitamins using strong acids and enzyme hydrolysis. [19][20][21][22][23][24][25][26][27][28][29][30] However, these tedious, complex and harsh methods are not necessary to extract the vitamins from vitamin-supplemented food products.…”

Section: Resultsmentioning

confidence: 99%

“…[19][20][21][22]24,25,27,30 Interference in the chromatograms and poor recovery indicate degradation of some vitamins due to the harsh acid. Organic solvents can also be used to precipitate proteins and are milder toward the samples.…”

Section: Resultsmentioning

confidence: 99%

“…Extraction techniques based on different acid treatments and a These authors contributed equally to this work. enzymatic digestion such as hydrochloric acid, sulfuric acid, perchloric acid or amylase and taka-diastase [19][20][21][22][23][24][25][26][27][28][29][30] have been developed, but the acids or enzymes require a long incubation (normally over 10-h) and high heat. Thus, these processes may decrease the recovery of unstable vitamins, such as folic acid.…”

Section: -17mentioning

confidence: 99%

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Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Suh¹,

Yang²,

Lee³

et al. 2011

Bulletin of the Korean Chemical Society

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A simple HPLC-DAD method was developed and validated to determine B group vitamin content (thiamine, riboflavin, nicotinamide, pantothenic acid, pyridoxine and folic acid) in supplemented food samples, i.e., infant formula, cereal, low-calorie food, a multi-vitamin pill and a vitamin drink. In this study, the most significant advantages were simultaneous determination of the six B group vitamins in various food matrices and a small number of sample treatment steps that required only an organic solvent, acetonitrile. Moreover, this method prevents reduction of column durability, because the mobile phase does not contain ion-pairing reagents. Analytes were separated on a Develosil RPAQUEOUS C 30 (4.6 mm × 250 mm, 5 µm particle size) column with a gradient elution of acetonitrile and 20 mM phosphate buffer (pH 3.0) at a flow rate between 0.8 and 1.0 mL/min. Detection was performed at 275 nm, except for that of pantothenic acid (205 nm). The calibration curves for all six vitamins showed good linearity with correlation coefficients (r 2 ) higher than 0.995. The developed method was validated with respect to linearity, intra-and inter-day accuracy and precision, limit of quantification (LOQ), recovery and stability. The method showed good precision and accuracy, with intra-and inter-assay coefficients of variation less than 15% at all concentrations. The recovery was carried out according to the standard addition procedure, with yields ranging from 89.8 to 104.4%. This method was successfully applied to the determination of vitamin B groups in supplemented food products.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: -17mentioning

confidence: 99%

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Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Suh¹,

Yang²,

Lee³

et al. 2011

Bulletin of the Korean Chemical Society

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“…HPLC was coupled with UV/VIS [14][15][16][17], fluorescence [12] or MS detection [18,19]. There have also been some experiments employing electrochemical detectors.…”

Section: Introductionmentioning

confidence: 99%

Using of HPLC coupled with coulometric detector for the determination of biotin in pharmaceuticals

Zerzaňová

Žižkovský

Kučera

et al. 2007

Journal of Pharmaceutical and Biomedical Analysis

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“…Especially, it is very effective in the analysis of compounds from complex samples because of its low detection limit, high sensitivity and the possibility for short run time [31][32][33]. Signal suppression or enhancement of the target extracts by matrix components is a common phenomenon in LC-MS/MS analysis and should be considered [34]. Moreover, interfering matrix components can affect accuracy of the proposed method and maybe lead to some compromising or erroneous results [35].…”

Section: Introductionmentioning

confidence: 99%

Rapid and quantitative determination of solanesol in Nicotiana tabacum by liquid chromatography–tandem mass spectrometry

Zhao

2007

Journal of Pharmaceutical and Biomedical Analysis

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Quantification of biotin in feed, food, tablets, and premixes using HPLC–MS/MS

Cited by 51 publications

References 11 publications

Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Simultaneous Determination of B Group Vitamins in Supplemented Food Products by High Performance Liquid Chromatography-Diode Array Detection

Using of HPLC coupled with coulometric detector for the determination of biotin in pharmaceuticals

Rapid and quantitative determination of solanesol in Nicotiana tabacum by liquid chromatography–tandem mass spectrometry

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