Rapid and quantitative determination of solanesol in Nicotiana tabacum by liquid chromatography–tandem mass spectrometry

Zhao, Chunjian; Li, Chunying; Zu, Yuangang

doi:10.1016/j.jpba.2007.01.021

Cited by 18 publications

(4 citation statements)

References 38 publications

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“…The amount of solanesol content in the tomato leaves is gradually increased until the leaf maturation and it also depends on the variety and conditions that plants grow [34]. The result of our preliminary studies and previous studies showed that the amount of solanesol in the leaves was nearly 6-fold higher than other parts of the plant [35, 36]. Therefore, in this study we assessed the effects of extraction period and solid to solvent ratio on the recovery of solanesol from tomato leaves.…”

Section: Resultsmentioning

confidence: 64%

A benign process for the recovery of solanesol from tomato leaf waste

Arab

Bahramian

Fathi

et al. 2019

Heliyon

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Solanesol, the precursor for the synthesis of coenzyme Q10, is currently recovered from tobacco leaves by conventional extraction techniques that require multiple purification steps and a large amount of organic solvents. We recently identified tomato leaves as an alternative source of solanesol and hypothesized that a high-pressure CO 2 extraction could be used as a clean extraction process. The effect of CO 2 pressure and temperature on the extraction of solanesol was determined to achieve high yield and purity. It was found that solanesol could be extracted efficiently by subcritical CO 2 at 25 °C from tomato leaves. The extract contained 40% solanesol and other active compounds such as vitamin K1. A higher level of purity of 93% was achieved using a secondary purification step. Different conventional methods for solanesol extraction was compared to determine the most efficient technique for production of solanesol from tomato leaf. The highest yield of solanesol was achieved at nearly 1% dry weight with using subcritical CO 2 , which was superior to conventional methods.

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Section: Resultsmentioning

confidence: 64%

A benign process for the recovery of solanesol from tomato leaf waste

Arab

Bahramian

Fathi

et al. 2019

Heliyon

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“…LC-MS has been widely used in the determination and analysis of solanesol. Zhao et al [67] utilized liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the determination of free solanesol in tobacco: the sample was prepared by ultrasonic extraction with methanol, liquid-liquid extraction, concentration, reconstitution, and filtration, followed by separation with the Symmetry Shield™ RP18 column. A mixture of acetonitrile-isopropanol (50:50, V/V) containing 2 mM ammonium acetate was used as mobile phase, and the determination was performed in positive-ion mode.…”

Section: Determination Methods Of Solanesolmentioning

confidence: 99%

Bioactivities and Medicinal Value of Solanesol and Its Accumulation, Extraction Technology, and Determination Methods

Ning

Liu

et al. 2019

Biomolecules

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Solanesol, an aliphatic terpene alcohol composed of nine isoprene units, is mainly found in solanaceous plants. Particularly, tobacco (Nicotiana tabacum), belonging to the Solanaceae family, is the richest plant source of solanesol, and its leaves have been regarded as the ideal material for solanesol extraction. Since the discovery of solanesol in tobacco, significant progress has been achieved in research on solanesol’s bioactivities, medicinal value, accumulation, extraction technology, and determination methods. Solanesol possesses strong free radical absorption ability and antioxidant activity owing to the presence of several non-conjugated double bonds. Notably, solanesol’s anti-inflammatory, neuroprotective, and antimicrobial activities have been previously demonstrated. Solanesol is a key intermediate in the synthesis of coenzyme Q10, vitamin K2, and the anticancer agent synergiser N-solanesyl-N,N′-bis(3,4-dimethoxybenzyl) ethylenediamine. Other applications of solanesol include solanesol derivative micelles for hydrophobic drug delivery, solanesol-derived scaffolds for bioactive peptide multimerization, and solanesol-anchored DNA for mediating vesicle fusion. Solanesol accumulation in plants is influenced by genetic and environmental factors, including biotic stresses caused by pathogen infections, temperature, illumination, and agronomic measures. Seven extraction technologies and seven determination methods of solanesol are also systematically summarized in the present review. This review can serve as a reference for solanesol’s comprehensive application.

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“…The content of solanesol in tobacco leaves is higher than that in any other organ [11]. A few methods for determination of solanesol in tobacco by HPLC [12][13], by GC [14], by HPLC-APCI-MS [15], and by LC-TMS [16], etc. have been reported.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of HPTLC Method for Quantification of Solanesol in Various Parts of <i>Nicotiana</i> <i>tabacum</i> Collected from Different Geographical Regions of India

Kaushik

Asif

2018

IJPPE

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Abstract. Solanesol is the starting material for many high value biochemicals, including Coenzyme Q10 and vitamin-K analogues. The aim of the current study was to develop and validate a reliable and fast analytical procedure for the determination of solanesol in Nicotiana tabacum using high-performance thin layer chromatography (HPTLC) method. The method was developed on TLC aluminium plates precoated with silica gel 60F-254 using solvent system hexane: ethyl acetate (5:1, v/v), which gives compact spot of solanesol (Rf value 0.41 ± 0.02). Densitometric analysis of solanesol was carried out in the absorbance mode at 210 nm. The linear regression analysis data for the calibration plot showed good linear relationship with r = 0.9978 with respect to peak area, in the concentration rang 100-5000 ng per spot of solanesol. The limit of detection and quantification were 13 and 30 ng per spot, respectively. The proposed method was applied for quantitative estimation of solanesol in different parts of Nicotiana tabacum from different geographical regions in India, which showed that maximum amount of solanesol was found to be present in leaf sample collected from Karnataka i.e. 3.52 mg/g. Statistical analysis proved that the method is repeatable, selective and accurate for the estimation of solanesol in Nicotiana tabacum.

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Rapid and quantitative determination of solanesol in Nicotiana tabacum by liquid chromatography–tandem mass spectrometry

Cited by 18 publications

References 38 publications

A benign process for the recovery of solanesol from tomato leaf waste

A benign process for the recovery of solanesol from tomato leaf waste

Bioactivities and Medicinal Value of Solanesol and Its Accumulation, Extraction Technology, and Determination Methods

Development and Validation of HPTLC Method for Quantification of Solanesol in Various Parts of <i>Nicotiana</i> <i>tabacum</i> Collected from Different Geographical Regions of India

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