Quantification of candidate prostate cancer metabolite biomarkers in urine using dispersive derivatization liquid–liquid microextraction followed by gas and liquid chromatography–mass spectrometry

Shamsipur, Mojtaba; Naseri, Mahmood; Babri, Mehran

doi:10.1016/j.jpba.2013.03.019

Cited by 49 publications

(32 citation statements)

References 30 publications

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“…When compared to other methods used for sarcosine separation and detection, such as LC–MS, used by Meyer et al with LOD of 5 nM , HPLC–MS/MS used by Jiang et al with an LOD of 3 nM . Using a method of derivatization with liquid–liquid microextraction in combination with LC–MS as well as GC–MS, Shamsipur et al reached a LOD of 1 nM . Although commonly used MS analysis shows better LODs, there is the advantage of possible electrochemical detector miniaturization, offering the option of detection via self‐standing sensors based on paramagnetic particles.…”

Section: Resultsmentioning

confidence: 99%

Preconcentration based on paramagnetic microparticles for the separation of sarcosine using hydrophilic interaction liquid chromatography coupled with coulometric detection

et al. 2014

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Sarcosine has been identified as a potential prostate cancer marker. To provide determination of this compound, a number of methods are developing. In this study, we optimized a method for its separation by hydrophilic interaction LC with electrochemical detection (ED). Due to the fact that mobile phases commonly used for this type of separation altered the LODs measured by electrochemical detectors, we applied postcolumn dosing of buffer suitable for ED. The optimized conditions were mobile phase A acetonitrile, mobile phase B water in the ratio A/B 70:30, with postcolumn addition of mobile phase C (200 mM phosphate buffer pH 9). The optimal mixing ratio was A + B/C 1:1 with a flow rate of 0.80 mL/min (0.40 + 0.40 mL/min) and detection potential of 1000 mV. Due to the optimization of the parameters for effective separation, which had to meet the optimal parameters of ED, we reached a good resolution for separation also with a good LOD (100 nM). In addition, we successfully carried out sarcosine analysis bound on our modified paramagnetic microparticles with the ability to preconcentrate sarcosine isolated from artificial urine.

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Section: Resultsmentioning

confidence: 99%

Preconcentration based on paramagnetic microparticles for the separation of sarcosine using hydrophilic interaction liquid chromatography coupled with coulometric detection

et al. 2014

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“…After centrifuging at 13,000×g for 15 min at 4°C, the deproteinized serum was collected and diluted 26 times, before analysis. Finally, the obtained results were compared with the those obtained from a method based on dispersive derivatization liquid-liquid microextraction (DDLLME) combined with gas chromatography-mass spectrometry (GC-MS) [17]. All fluorescence measurements were performed at room temperature under ambient conditions.…”

Section: Synthesis Of Hemoglobin-capped Auncsmentioning

confidence: 99%

“…Consequently, based on the necessary information obtained from the corresponding Förster relation and equation mentioned in ESM, we calculated k F as 6.07×10 7 s −1 , J as 7.14×10 16 M −1 cm −1 , E as 0.135, and R 0 as 65.1 Å. Such short R 0 value is an indication for efficient energy transfer between the donor-acceptor pair [17,29].…”

Section: Study Of Fluorescence Resonance Energy Transfer (Fret) From mentioning

confidence: 99%

Novel blue-emitting gold nanoclusters confined in human hemoglobin, and their use as fluorescent probes for copper(II) and histidine

Shamsipur

Molaabasi

Shanehsaz³

et al. 2014

Microchim Acta

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We report on the synthesis and characterization of highly fluorescent gold nanoclusters (Au-NCs) from a gold precursor using a single-step chemical reduction in the presence of human adult hemoglobin (Hb). The conformational changes of Hb before and after cluster formation were studied by various spectroscopic techniques. The Au-NCs in Hb display blue fluorescence with a peak centered at 450 nm (photoexcited at 365 nm) and a quantum yield of 2.8 %. The Au-NCs exhibit excellent photostability and long-term stability, and can be applied in the pH range 5-12 even in the presence of high electrolyte concentrations. The Au-NCs in Hb can act as a highly sensitive and selective fluorescent turnoff probe for Cu(II) ion. The observed reversible fluorescence recovery of Hb-AuNCs/Cu(II) aggregates was exploited to develop a selective and sensitive turn-on fluorescence assay for His. Under optimized conditions, the probe gives a fluorescent response that is linear in the 0.1 to 20.0 μM concentration range of Cu(II), with a limit of detection of 28 nM. The probe for His, in turn, has a linear range in the 1-21 μM concentration range, and the limit of detection is 0.6 μM.

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“…Several metabolic disorders and diseases can be diagnosed by determining amino acid concentration levels [2][3][4][5][6]. Although some amino acids have also been studied and proposed as candidate cancer biomarkers [7][8][9][10][11][12][13][14][15][16][17], their validity as cancer biomarkers remains unclear, and more studies and analysis of a large number of samples are needed [18]. However, the development of new analytical methods is a very important point for the research and discovery of sensitive biomarkers and for early disease detection [18,19].…”

Section: Introductionmentioning

confidence: 99%

Derivatization coupled to headspace programmed‐temperature vaporizer gas chromatography with mass spectrometry for the determination of amino acids: Application to urine samples

Paredes

Pinto

Pavón

et al. 2016

J of Separation Science

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A new method based on headspace programmed-temperature vaporizer gas chromatography with mass spectrometry has been developed and validated for the determination of amino acids (alanine, sarcosine, ethylglycine, valine, leucine, and proline) in human urine samples. Derivatization with ethyl chloroformate was employed successfully to determine the amino acids. The derivatization reaction conditions as well as the variables of the headspace sampling were optimized. The existence of a matrix effect was checked and the analytical characteristics of the method were determined. The limits of detection were 0.15-2.89 mg/L, and the limits of quantification were 0.46-8.67 mg/L. The instrumental repeatability was 1.6-11.5%. The quantification of the amino acids in six urine samples from healthy subjects was performed with the method developed with the one-point standard additions protocol, with norleucine as the internal standard.

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Quantification of candidate prostate cancer metabolite biomarkers in urine using dispersive derivatization liquid–liquid microextraction followed by gas and liquid chromatography–mass spectrometry

Cited by 49 publications

References 30 publications

Preconcentration based on paramagnetic microparticles for the separation of sarcosine using hydrophilic interaction liquid chromatography coupled with coulometric detection

Preconcentration based on paramagnetic microparticles for the separation of sarcosine using hydrophilic interaction liquid chromatography coupled with coulometric detection

Novel blue-emitting gold nanoclusters confined in human hemoglobin, and their use as fluorescent probes for copper(II) and histidine

Derivatization coupled to headspace programmed‐temperature vaporizer gas chromatography with mass spectrometry for the determination of amino acids: Application to urine samples

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