Quantification of Carbohydrates and Related Materials Using Sodium Ion Adducts Produced by Matrix-Assisted Laser Desorption Ionization

Abstract

Abstract. The utility of sodium ion adducts produced by matrix-assisted laser desorption ionization for the quantification of analytes with multiple oxygen atoms was evaluated. Uses of homogeneous solid samples and temperature control allowed the acquisition of reproducible spectra. The method resulted in a direct proportionality between the ion abundance ratio I([A + Na] + )/I([M + Na] + ) and the analyte concentration, which could be used as a calibration curve. This was demonstrated for carbohydrates, glycans, and polyether diols with dynamic range exceeding three orders of magnitude.

Analysis of Carbohydrates and Glycoconjugates by Matrix‐assisted Laser Desorption/Ionization Mass Spectrometry: An Update for 2015–2016

“…Nevertheless, the authors found that the use of these ions from both sample and matrix produced similar results for quantification. Calibration curves were essentially linear over three orders of magnitude with results reported from stachyose ( 1/19 ), and the N ‐glycans Man 5 GlcNAc 2 ( 39 ) and a fucosylated biantennary complex glycan ( 40 ) (Ahn et al, 2016).

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KatG-Mediated Oxidation Leading to Reduced Susceptibility of Bacteria to Kanamycin

“…The sample was incubated with (b) 1 μg/mL KatG for 15 min and (c) 1 μg/mL KatG supplemented with 5 mg/mL glucose oxidase and 5 mM glucose for 15 min. Sodium ion adducts of carbohydrates are a well-documented product of MALDI …”