Quantification of diethylstilbestrol residues in meat samples by gas chromatography-isotope-dilution mass spectrometry

Peteghem, Carlos H. Van; Lefevere, M.F.; Haver, G.M. Van; Leenheer, A.P. De

doi:10.1021/jf00074a015

Cited by 22 publications

(1 citation statement)

References 13 publications

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“…1 In the literature several other methods for the detection of one or of a group of substances, e.g., oestrogens in biological matrices, have been published. [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] There is, however, a need for validated multi-residue methods for the detection of anabolic steroids, in which new compounds, introduced on the black market, can be integrated. Another necessity in the framework of accreditation is validation of the developed methods and of the results obtained.…”

Section: Introductionmentioning

confidence: 99%

Validation of multi-residue methods for the detection of anabolic steroids by GC-MS in muscle tissues and urine samples from cattle†

Daeseleire¹,

Vandeputte²,

Peteghem³

1998

Analyst

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The use of anabolic steroids is prohibited in the European Community by Directive 96/22/EC and the control of compliance is regulated by Directive 96/23/EC. Multi-residue methods are necessary for screening for the use of forbidden substances. Because accreditation is gaining more and more importance, validation of the methods used and of the results obtained has become indispensable. The developed GC-MS methods for the detection of anabolic steroids in urine and muscle tissue were validated with regard to the following parameters: specificity, recovery at the 2 micrograms kg-1 level and limit of detection. For urine the recoveries ranged from 17 to 81% and for muscle tissue from 26 to 65%. The limit of detection ranged from 0.1 to 2.6 micrograms kg-1 for urine and from 0.3 to 4.6 micrograms kg-1 for muscle tissue. Specificity was guaranteed in both matrices by the selection of four specific ions. Blank samples were evaluated for interferences and it could be concluded that in no case did the four selected ions appear simultaneously at the correct retention time. The practicability of the criteria for low resolution mass spectrometry set in Decision 93/256 in the low micrograms kg-1 range is also briefly discussed.

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Section: Introductionmentioning

confidence: 99%

Validation of multi-residue methods for the detection of anabolic steroids by GC-MS in muscle tissues and urine samples from cattle†

Daeseleire¹,

Vandeputte²,

Peteghem³

1998

Analyst

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Development of Monoclonal Antibody‐based Enzyme‐linked Immunosorbent Assay to the Estrogen Diethylstilbestrol

Wang

Li²,

Zhao

et al. 2006

Chin. J. Chem.

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Diethylstilbestrol (DES) is a synthetic estrogen that has ever been used worldwide. Polyclonal antibodies (PAbs) were used in immunoassay for detection of DES residues in environmental and agricultural samples in previous paper. In this paper, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed based on monoclonal antibody (MAb) for the determination of diethylstilbestrol. Mono-o-carboxypropyldiethylstilbestrol (DES-CP) and mono-o-carboxymethyldiethylstilbestrol (DES-CME) were synthesized to be haptens. DES-CP was coupled to bovine serum albumin (BSA) to be an immunogen in BALB/c female mouse for MAb production. The MAb was characterized for specificity and affinity to DES in icELISA. Under the optimum condition, the icELISA showed an IC 50 of 9.8 ng/mL, the limit of detection (IC 20 ) of 2.3 ng/mL and a working range of 2-42 ng/mL. Hexestrol and dienestrol exhibited cross-reactivity values were 44% and 27%, respectively. Cross-reactivity of natural estrogen 17β-estradiol was less than 0.1%. The influences of some factors such as salt concentration, pH and organic solvent concentration on the assay were evaluated. The concentrations of DES in the fortified water samples determined by the assay were correlated well with the fortification levels. The results were confirmed with analysis by HPLC.

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On‐line high performance liquid chromatography — multidimensional gas chromatography and its application to the determination of stilbene hormones in corned beef

Chappell

Creaser

Shepherd

1993

J. High Resol. Chromatogr.

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SummaryThe combination of high performance liquid chromatography interfaced on-line with multidimensional gas chromatography (HPLC-GC-GC) is described. The HPLC column was interfaced to the GC via an on column interface, with automated pneumatic control of solvent evaporation and GC column switching. Cryogenic cold trapping was used for analyte focusing at the head of the first, non-polar GC capillary column and optionally at the head of the second, polar column. The determination of stilbene hormones in corned beef as their methylated derivatives by flame ionization detection i s described.

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Quantification of diethylstilbestrol residues in meat samples by gas chromatography-isotope-dilution mass spectrometry

Cited by 22 publications

References 13 publications

Validation of multi-residue methods for the detection of anabolic steroids by GC-MS in muscle tissues and urine samples from cattle†

Validation of multi-residue methods for the detection of anabolic steroids by GC-MS in muscle tissues and urine samples from cattle†

Development of Monoclonal Antibody‐based Enzyme‐linked Immunosorbent Assay to the Estrogen Diethylstilbestrol

On‐line high performance liquid chromatography — multidimensional gas chromatography and its application to the determination of stilbene hormones in corned beef

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