Quantification of ethanol using a luminescence system derived from Photobacterium leiognathi

Abstract

We established a method based on a luminescence system driven by NADH conversion to quantitatively detect ethanol in alcoholic beverages. NADH produced by an ADH-catalyzed enzymatic reaction was used as an indicator to directly quantify luminescence in vitro. A strong linear relationship (R 2 ¼ 0.9942) was observed between the luminescence intensity and ethanol concentration over a range of 0.002-0.1 mol L À1 , providing a detection limit of 1.43 Â 10 À4 mol L À1 for ethanol. This system has advantages such as rapid detection, high specificity, and repeatability in operation; therefore, it could be an advantageous alternative method for accurate quantification of ethanol content in beer, and may be suitable for other applications in the food industry.

Rapid determination of ethanol content based on an optical fiber-device and R6G-indicator

“…2,3 In addition, other detecting methods could not meet the demands for rapid and on-site ethanol determination. For example, in chromatography, 4,5 the operation is complex and the equipment is heavy and expensive; in the enzyme method, the stability of the puried enzymes is poor, 6 which leads to low repeatability and accuracy; in near-infrared spectroscopy, the process is timeconsuming and calibration is sophisticated. 7,8 In recent years, some detection methods, based on uorescence, have been proposed.…”