Quantification of isoorientin in Passiflora edulis rinds by HPTLC-densitometry and HPLC/DAD methods and evaluation of radical scavenging capacity of the extracts

Zeraik, ML; Wauters, Jean-Noël; Angenot, Luc; Yariwake, JH

doi:10.1055/s-0033-1352256

Cited by 2 publications

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“…SILVA et al, 2014;ICHIMURA et al, 2006;ZIBADI et al, 2007). Nos últimos anos, alguns estudos trazem informações sobre os possíveis benefícios da farinha do maracujá, a qual é produzida com a casca do fruto, indicando esta como um alimento funcional (DA SILVA et al, 2014;JANEBRO et al, 2008;RAMOS et al, 2007 Os flavonoides foram as moléculas mais estudadas, sendo que os flavonoides C-glicosilados, como iso-orientina, orientina, vitexina e isovitexina, se destacam por conta de suas altas capacidades antioxidantes (ZERAIK et al, 2013). O grupo de alcaloides presente nessas espécies de Passiflora é o grupo dos alcaloides β-carbolínicos.…”

Section: Freire V F Estudo Dos Alcaloides β-Carbolínicos Dos Frutounclassified

Estudo de alcaloides β-carbolínicos dos frutos de Passiflora alata e de Passiflora edulis utilizando SBSE, LC/Flu e LC/MS

Freire¹

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AGRADECIMENTOSAgradeço imensamente a todos que contribuíram para minha formação ao longo desses dois anos de mestrado.Começo agradecendo muito ao meu pai, Petronilio, minha mãe, Tania, e minha irmã, Dayane, por todo o apoio demonstrado ao longo de meu caminho até aqui.Não posso deixar de agradecer minha orientadora, Janete, que tanto auxiliou em minha formação, sendo uma orientadora muito presente, sempre disposta a discutir e me questionando sobre cada aspecto do trabalho. Ao técnico do laboratório, Benedito, um amigo que fiz durante o mestrado, e que me ajudou muito no desenvolvimento do trabalho. À Renata Colombo, que sempre esteve disposta a ajudar como fosse possível ao decorrer do trabalho. Ao Leandro Arriveti pelo auxílio nas análises por LC/MS.Agradeço também a todos meus amigos, aos antigos, que continuaram muito presentes ao longo desses dois anos e aos que fiz durante esse período do mestrado. Em especial, gostaria de agradecer a parceria de uma pessoa com quem já tinha amizade desde a época de graduação, mas que durante o mestrado se tornou uma pessoa importantíssima nesse tempo em São Carlos, a grande amiga Mirelle Takaki.Agradeço ao CNPq pela bolsa concedida (Processo: 131094/2015-7) e à FAPESP pelo auxílio financeiro ao projeto (Processo: 21886-7/2013) alkaloids is a minor component of these fruits, however, the literature indicates that substances belonging to this group may have toxic activity. This work presents three specific objectives, in order to increase knowledge about the phytochemistry of P. edulis and P. alata and the application of SBSE as a sample preparation method:quantification of harmane and harmine in the pulp and in the seeds of P. alata using SBSE-PDMS and HPLC/Flu analysis; identification of β-carboline alkaloids in P. edulis peels using HPLC/Flu and UHPLC/MS analysis; study of the SBSE extraction process of norharmane using two different phases (PDMS and EGSilicone), by HPLC/Flu analysis. Quantification of harmane and harmine in the pulp and the seeds of P. alata was performed after sample preparation by SBSE-PDMS and analysis by HPLC/Flu. The concentration of harmane was 1,0328 . 10 -1 ± 3,1217 . 10 -3 µg L -1 in the pulp while the in the seeds it was found 7.4391 . 10 -5 ± 2.5501 . 10 -6 µg g -1 harmane. The concentrations of harmine were below the LOD and LOQ in the pulp and in the seeds of P. alata, respectively. For the identification of the β-carboline alkaloids in the peels of P. edulis, the extracts were prepared by using the classic method and analyzed by HPLC/Flu and UHPLC/MS, and the alkaloids norharmane, harmane, harmine, harmol and harmalol were identified. In the HPLC/Flu analysis of the extract of P. edulis peels prepared using SBSE-PDMS, the alkaloid norharmane was identified as a major constituent and, for this reason, it was performed a study about the extraction process of norharmane by SBSE, evaluating two different phases, PDMS and EG-Silicone, by using fractional factorial design, aiming to get the best percentage recovery. Defined the best condition...

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Section: Freire V F Estudo Dos Alcaloides β-Carbolínicos Dos Frutounclassified

Estudo de alcaloides β-carbolínicos dos frutos de Passiflora alata e de Passiflora edulis utilizando SBSE, LC/Flu e LC/MS

Freire¹

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A systematic review on phenolic compounds inPassifloraplants: Exploring biodiversity for food, nutrition, and popular medicine

Gadioli

Cunha

Carvalho

et al. 2017

Critical Reviews in Food Science and Nutrition

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Passiflora plants are strategic in the context of biodiversity for food and nutrition. We applied the procedures of a systematic review protocol to study the state of the art on identification of phenolic compounds from Passiflora plants. An automated literature search was conducted using six databases and a combination of seven keywords. All the analytical, chromatographic, and spectroscopic methods were included. The studies were classified according to their method of identification, phenolic classes, and method of extraction. In total, 8,592 abstracts were found, from which 122 studies were selected for complete reading and 82 were selected for further analysis. Techniques of extraction, evaluated parts of the plant and methods of identification were systematized. Studies with leaves were most conspicuous (54.4%), 34 species of Passiflora were evaluated and orientin, isoorientin, vitexin, isovitexin were commonly found structures. A High Performance Liquid Chromatography-diode array detector was the technique most applied, with which the same structures were identified all through the studies, although other unknown structures were detected, but not elucidated. The use of Nuclear Magnetic Resonance and Mass Spectrometry, which are more sensitive techniques, needs to be intensified, to identify other unconventional compounds detected in Passiflora, to enhance the comprehension of the bioactive compounds in these plants.

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Quantification of isoorientin in Passiflora edulis rinds by HPTLC-densitometry and HPLC/DAD methods and evaluation of radical scavenging capacity of the extracts

Cited by 2 publications

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Estudo de alcaloides β-carbolínicos dos frutos de <i>Passiflora alata</i> e de <i>Passiflora edulis</i> utilizando SBSE, LC/Flu e LC/MS

Estudo de alcaloides β-carbolínicos dos frutos de <i>Passiflora alata</i> e de <i>Passiflora edulis</i> utilizando SBSE, LC/Flu e LC/MS

A systematic review on phenolic compounds inPassifloraplants: Exploring biodiversity for food, nutrition, and popular medicine

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Quantification of isoorientin in Passiflora edulis rinds by HPTLC-densitometry and HPLC/DAD methods and evaluation of radical scavenging capacity of the extracts

Cited by 2 publications

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Estudo de alcaloides &#946;-carbolínicos dos frutos de <i>Passiflora alata</i> e de <i>Passiflora edulis</i> utilizando SBSE, LC/Flu e LC/MS

Estudo de alcaloides &#946;-carbolínicos dos frutos de <i>Passiflora alata</i> e de <i>Passiflora edulis</i> utilizando SBSE, LC/Flu e LC/MS

A systematic review on phenolic compounds inPassifloraplants: Exploring biodiversity for food, nutrition, and popular medicine

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Estudo de alcaloides β-carbolínicos dos frutos de <i>Passiflora alata</i> e de <i>Passiflora edulis</i> utilizando SBSE, LC/Flu e LC/MS

Estudo de alcaloides β-carbolínicos dos frutos de <i>Passiflora alata</i> e de <i>Passiflora edulis</i> utilizando SBSE, LC/Flu e LC/MS