Quantification of Lung Damage in an Elastase-Induced Mouse Model of Emphysema

Muñoz-Barrutia, Arrate; Ceresa, Mario; Artaechevarria, Xabier; Montuenga, Luis M.; Ortiz‐de‐Solórzano, Carlos

doi:10.1155/2012/734734

Cited by 50 publications

(47 citation statements)

References 24 publications

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“…Histological and CT analyzes are intended for distinct purposes. For instance, histological assessment of lung tissue enables the evaluation of treatments and could be used as a reference for quantification techniques based on non-invasive procedures such as CT. 17,19 The main advantage of CT is given by its non-invasive nature, making possible the assessment of living individuals. Yet, the radiation exposure is a clear drawback of this technique.…”

Section: Discussionmentioning

confidence: 99%

“…12,22 Therefore, given an area of lung tissue, the first step to rank emphysema severity by means of SI is the computation of D 2 . For this purpose, an automated procedure similar to that described by Mun˜oz-Barrutia et al 19 was applied. It encompasses the following operations:…”

Section: Emphysema Severity Indexmentioning

confidence: 99%

“…The 8-bit grayscale green channel is extracted from the 24-bit RGB image of the lung area since it provides the greatest contrast between the background and the red-blue H&E stained tissue. 12,19 2. The image is binarized using the Otsu's method in order to separate the parenchymal tissue from the airspaces.…”

Section: Emphysema Severity Indexmentioning

confidence: 99%

“…Therefore, artifact rejection was based on the identification of abnormally thick lines in the image. 19 Once one of these thick borders is detected, we took into account that it might define, or not, a closed region. A specific procedure based on the identification of concavities was then designed to fill open regions.…”

Section: Calculation Of the Emphysema Mapmentioning

confidence: 99%

“…12 Indeed, D 2 has been used as the gold-standard metric for airspace enlargement quantification in the study of gas flow inside the lung tissue 15 or in the development of novel in vivo techniques of emphysema analysis based on microcomputed tomography (micro-CT). 2,19 It has been reported that larger values of D 2 are expected for areas of parenchymal tissue showing an increased enlargement of the airspaces as a consequence of emphysematous lesions. 22 However, while the value of D 2 provides a measure of airspace enlargement, it does not reflect emphysema severity in a given lung area.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Assessment of Emphysema Severity in Histological Lung Analysis

et al. 2015

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Abstract-Emphysema is a characteristic component of chronic obstructive pulmonary disease (COPD), which has been pointed out as one of the main causes of mortality for the next years. Animal models of emphysema are employed to study the evolution of this disease as well as the effect of treatments. In this context, measures such as the mean linear intercept and the equivalent diameter ðdÞ have been proposed to quantify the airspace enlargement associated with emphysematous lesions in histological sections. The parameter D 2 , which relates the second and the third moments of the variable d, has recently shown to be a robust descriptor of airspace enlargement. However, the value of D 2 does not provide a direct evaluation of emphysema severity. In our research, we suggest a Bayesian approach to map D 2 onto a novel emphysema severity index (SI) reflecting the probability for a lung area to be emphysematous. Addition-ally, an image segmentation procedure was developed to compute the severity map of a lung section using the SI function. Severity maps corresponding to 54 lung sections from control mice, mice induced with mild emphysema and mice induced with severe emphysema were computed, revealing differences between the distribution of SI in the three groups. The proposed methodology could then assist in the quantification of emphysema severity in animal models of pulmonary disease.

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Section: Discussionmentioning

confidence: 99%

Section: Emphysema Severity Indexmentioning

confidence: 99%

Section: Emphysema Severity Indexmentioning

confidence: 99%

Section: Calculation Of the Emphysema Mapmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantitative Assessment of Emphysema Severity in Histological Lung Analysis

et al. 2015

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Perfluorochemical‐facilitated plasminogen activator delivery to the airways: A novel treatment for inhalational smoke‐induced acute lung injury

Wolfson

Enkhbaatar

Fukuda

et al. 2020

Clinical & Translational Med

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Background Effective clinical management of airway clot and fibrinous cast formation of severe inhalational smoke‐induced acute lung injury (ISALI) is lacking. Aerosolized delivery of tissue plasminogen activator (tPA) is confounded by airway bleeding; single‐chain urokinase plasminogen activator (scuPA) moderated this adverse effect and supported transient improvement in gas exchange and lung mechanics. However, neither aerosolized plasminogen activator (PA) yielded durable improvements in physiologic responses or reduction in cast burden. Here, we hypothesized that perfluorochemical (PFC) liquids would facilitate PA distribution and sustain improvements in physiologic outcomes in ISALI. Methods Spontaneously breathing adult sheep (n = 36) received anesthesia and analgesia and were instrumented, exposed to cotton smoke inhalation, and supported by mechanical ventilation for 48 h. Groups (n = 6/group) were studied without supplemental treatment, or, starting 4 h post injury, they received intratracheal low volume (8 mL) PFC liquid alone or a dose range of tPA/PFC or scuPA/PFC suspensions (4 or 8 mg in 8 mL PFC) every 8 h. Outcomes were evaluated by sequential measurements of cardiopulmonary parameters, lung histomorphology, and biochemical analyses of bronchoalveolar lavage fluid. Results Dose‐response and PA‐type comparisons of outcomes demonstrated sustained superiority with low‐volume PFC suspensions of scuPA over tPA or PFC alone, favoring the highest dose of scuPA/PFC suspension over lower doses, without airway bleeding. Conclusions We propose that this improved profile over previously reported aerosolized delivery is likely related to improved dose distribution. Sustained salutary responses to scuPA/PFC suspension delivery in this translational model are encouraging and support the possibility that the observed outcomes could be of clinical importance.

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Detection of early pulmonary emphysema by multi‐contrast x‐ray Talbot‐Lau interferometer

Lin,

Wu,

Huang

et al. 2024

Medical Physics

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BackgroundPulmonary emphysema is a part of chronic obstructive pulmonary disease, which is an irreversible chronic respiratory disease. In order to avoid further damage to lung tissue, early diagnosis and treatment of pulmonary emphysema is essential.PurposeEarly pulmonary emphysema diagnosis is difficult with conventional radiographic imaging. Recently, x‐ray phase contrast imaging has proved to be an effective and promising imaging strategy for soft tissue, due to its high sensitivity and multi‐contrast. The aim of this study is to diagnose pulmonary emphysema early utilizing an x‐ray Talbot‐Lau interferometer (TLI).MethodsWe successfully established the mouse model of emphysema by porcine pancreatic elastase treatment, and then used the established x‐ray TLI to perform imaging experiments on the mice with different treatment time. The traditional absorption CT and phase contrast CT were obtained simultaneously through TLI. The CT results and histopathology of mice lung in different treatment time were quantitatively analyzed.ResultsBy imaging mice lungs, it can be found that phase contrast has higher sensitivity than absorption contrast in early pulmonary emphysema. The results show that the phase contrast signal could distinguish the pulmonary emphysema earlier than the conventional attenuation signal, which can be consistent with histological images. Through the quantitative analysis of pathological section and phase contrast CT, it can be found that there is a strong linear correlation.ConclusionsIn this study, we quantitatively analyze mean linear intercept of histological sections and CT values of mice. The results show that the phase contrast signal has higher imaging sensitivity than the attenuation signal. X‐ray TLI multi‐contrast imaging is proved as a potential diagnostic method for early pulmonary emphysema in mice.

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Quantification of Lung Damage in an Elastase-Induced Mouse Model of Emphysema

Cited by 50 publications

References 24 publications

Quantitative Assessment of Emphysema Severity in Histological Lung Analysis

Quantitative Assessment of Emphysema Severity in Histological Lung Analysis

Perfluorochemical‐facilitated plasminogen activator delivery to the airways: A novel treatment for inhalational smoke‐induced acute lung injury

Detection of early pulmonary emphysema by multi‐contrast x‐ray Talbot‐Lau interferometer

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