Quantification of Marek's disease virus in chicken lymphocytes using the polymerase chain reaction with fluorescence detection

Bumstead, Nat; Sillibourne, Julie; Rennie, M.; Ross, Norman; Davison, Fred

doi:10.1016/s0166-0934(96)02172-6

Cited by 53 publications

(28 citation statements)

References 6 publications

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“…Bumstead et al (10) reported that the correlation between this PCR assay and plaque assays is high and suggested that MDV copy number per cell is rather uniform. It therefore seems reasonable to compare observations made in this study with the PCR-based assay with earlier findings that used plaque assays.…”

mentioning

confidence: 94%

“…This region has synteny with the mammalian lectin-like natural killer (NK) cell antigen complex (31,65) that contains the Cmv1 locus for resistance to murine cytomegalovirus (8,33,47). The outcome of MDV infection is associated with MDV load during the early stages of infection, a significant association between viral load in peripheral blood leukocytes and the development of clinical MD being evident as early as 4 dpi (10).…”

mentioning

confidence: 99%

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Differential Cytokine Responses following Marek's Disease Virus Infection of Chickens Differing in Resistance to Marek's Disease

Kaiser

Underwood

Davison

2003

J Virol

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187

114

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The production of cytokine mRNAs, in addition to viral DNA, was quantified by real-time quantitative reverse transcription-PCR (RT-PCR) (cytokines) or PCR (virus) in splenocytes during the course of Marek's disease virus (MDV) infection in four inbred chicken lines: two resistant (lines 6 1 and N) and two susceptible (lines 7 2 and P). Virus loads were only different after 10 days postinfection (dpi), increasing in susceptible lines and decreasing in resistant lines. Gamma interferon (IFN-␥) mRNA was expressed by splenocytes from all infected birds between 3 and 10 dpi, associated with increasing MDV loads. For other cytokines, differences between lines were only seen for interleukin-6 (IL-6) and IL-18, with splenocytes from susceptible birds expressing high levels of both transcripts during the cytolytic phase of infection, whereas splenocytes from resistant birds expressed neither transcript. These results indicate that these two cytokines could play a crucial role in driving immune responses, which in resistant lines maintain MDV latency but in susceptible lines result in lymphomas.

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mentioning

confidence: 94%

mentioning

confidence: 99%

Differential Cytokine Responses following Marek's Disease Virus Infection of Chickens Differing in Resistance to Marek's Disease

Kaiser

Underwood

Davison

2003

J Virol

Self Cite

187

114

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show abstract

“…In one, fluorescein-tagge d primers were detected by an automated sequencing machine (Bumstead et al, 1997;Burgess & Davison, 1999), one procedure including a PCR for chicken interferon gamma, as an internal standard and control (Burgess & Davison, 1999). An alternative approach has been described by Reddy et al (2000).…”

Section: Marek's Diseasementioning

confidence: 99%

Innovation and discovery: The application of nucleic acid-based technology to avian virus detection and characterization

Cavanagh¹

2001

Avian Pathology

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Polymerase chain reaction (PCR)-based approaches to the detection, differentiation and characterization of avian pathogens continue to be developed and refined. The PCRs, or reverse transcriptasePCRs, may be general, designed to detect all or most variants of a pathogen, or to be serotype, genotype or pathotype specific. Progress is being made with respect to making nucleic acid approaches more suitable for use in diagnostic laboratories. Robotic workstations are now available for extraction of nucleic acid from many samples in a short time, for routine diagnosis. Following general PCR, the DNA products are commonly analyzed by restriction endonuclease mapping (restriction fragment length polymorphism), using a small number of restriction endonucleases, based on a large body of sequence data. Increasingly, however, nucleotide sequencing is being used to analyze the DNA product, in part due to the expanding use of non-radioactive sequencing methods that are safe and enable high throughout. In this review, I highlight some recent developments with many avian viruses: Newcastle disease virus; circoviruses in canary and pigeon; infectious bursal disease virus (Gumboro disease virus); avian adenoviruses, including Angara disease/infectious hydropericardium virus, haemorrhagic enteritis virus of turkeys, and egg drop syndrome virus; avian herpesviruses, including infectious laryngotracheitis virus, duck plague virus, psittacine herpesvirus (Pacheco's parrot disease virus), Marek's disease virus and herpesvirus of turkeys; avian leukosis virus (associated with lymphoid leukosis or myeloid leukosis, and egg transmission); avian pneumoviruses (turkey rhinotracheitis virus); avian coronaviruses, including infectious bronchitis virus, turkey coronavirus and pheasant coronavirus; astrovirus, in the context of poult enteritis and mortality syndrome, and avian nephritis virus; and avian encephalomyelitis virus, a picornavirus related to hepatitis A virus.

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“…The speed and severity of these events largely depends on the virulence of virus, the genotype of host, and the maternal antibody and vaccination status of the host with regards to MD (Witter et al, 1980;Calnek et al, 1998). The severity of clinical MD is highly correlated with the number of copies of the viral genome present in host lymphoid cells (Bumstead et al, 1997;Lee et al, 1999). Viral load in the target cells at a given stage of infection is largely dependent on host genotype (Bumstead et al, 1997), vaccination status (Lee et al, 1999) and probably maternal antibody status of the host.…”

Section: Introductionmentioning

confidence: 99%

“…The severity of clinical MD is highly correlated with the number of copies of the viral genome present in host lymphoid cells (Bumstead et al, 1997;Lee et al, 1999). Viral load in the target cells at a given stage of infection is largely dependent on host genotype (Bumstead et al, 1997), vaccination status (Lee et al, 1999) and probably maternal antibody status of the host.…”

Section: Introductionmentioning

confidence: 99%

Marek's disease in broiler chickens: Effect of route of infection and herpesvirus of turkey-vaccination status on detection of virus from blood or spleen by polymerase chain reaction, and on weights of birds, bursa and spleen

Islam¹,

Walkden‐Brown²,

Burgess³

et al. 2001

Avian Pathology

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Quantification of Marek's disease virus in chicken lymphocytes using the polymerase chain reaction with fluorescence detection

Cited by 53 publications

References 6 publications

Differential Cytokine Responses following Marek's Disease Virus Infection of Chickens Differing in Resistance to Marek's Disease

Differential Cytokine Responses following Marek's Disease Virus Infection of Chickens Differing in Resistance to Marek's Disease

Innovation and discovery: The application of nucleic acid-based technology to avian virus detection and characterization

Marek's disease in broiler chickens: Effect of route of infection and herpesvirus of turkey-vaccination status on detection of virus from blood or spleen by polymerase chain reaction, and on weights of birds, bursa and spleen

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