Quantification of morphometric changes in murine experimental osteoarthritis using image analysis

Valburg, A. A. Van; Osch, Gerjo J. V. M. van; Kraan, P.M. van der; Berg, Wim B. van den

doi:10.1007/bf00290519

Cited by 9 publications

(4 citation statements)

References 19 publications

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“…Joints were embedded intact into paraffin with the patella down and the femur and tibia forming equal angles to the rim of the embedding cassette, ensuring that the joint was not rotated medially or laterally. Joints were then serially sectioned in a coronal plane, and 4 to 5 representative midcoronal 4-μm-thick sections that spanned approximately 100 μm were selected and stained as previously described 15 with hematoxylin & eosin (H&E) and Safranin-O.…”

Section: Methodsmentioning

confidence: 99%

“…A previous study developed a method to quantitate histological changes in murine stifle joints using image analysis on a single midcoronal section per joint and found that measurements of articular cartilage degradation and osteophyte size were reproducible. 15 Cellular parameters (e.g., chondrocyte numbers) and changes in subchondral bone were not evaluated.…”

Section: Introductionmentioning

confidence: 99%

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A Comprehensive Histological Assessment of Osteoarthritis Lesions in Mice

et al. 2011

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Objective:Accurate histological assessment of osteoarthritis (OA) is critical in studies evaluating the effects of interventions on disease severity. The purpose of the present study was to develop a histological grading scheme that comprehensively and quantitatively assesses changes in multiple tissues that are associated with OA of the stifle joint in mice.Design:Two representative midcoronal sections from 158 stifle joints, including naturally occurring and surgically induced OA, were stained with H&E and Safranin-O stains. All slides were evaluated to characterize the changes present. A grading scheme that includes both measurements and semiquantitative scores was developed, and principal components analysis (PCA) was applied to the resulting data from the medial tibial plateaus. A subset of 30 tibial plateaus representing a wide range of severity was then evaluated by 4 observers. Reliability of the results was evaluated using intraclass correlation coefficients (ICCs) and area under the receiver operating characteristic (ROC) curve.Results:Five factors were retained by PCA, accounting for 74% of the total variance. Interobserver and intraobserver reproducibilities for evaluations of articular cartilage and subchondral bone were acceptable. The articular cartilage integrity and chondrocyte viability factor scores were able to distinguish severe OA from normal, minimal, mild, and moderate disease.Conclusion:This newly developed grading scheme and resulting factors characterize a range of joint changes in mouse stifle joints that are associated with OA. Overall, the newly developed scheme is reliable and reproducible, characterizes changes in multiple tissues, and provides comprehensive information regarding a specific site in the stifle joint.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Comprehensive Histological Assessment of Osteoarthritis Lesions in Mice

et al. 2011

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“…It appeared to be possible with this method to measure the amount of cartilage loss in the medial and lateral tibial plateau reproducibly. Unfortunately, cartilage damage of the femoral condyle could not be measured with sufficient reproducibility [12].…”

Section: Quantification Proceduresmentioning

confidence: 99%

“…The amount of cartilage loss and the size of the osteophytes were quantified using a previously developed and validated method [ 12]. In short, the amount of cartilage loss was presented as a percentage of the total cartilage area in that compartment.…”

Section: Quantification Proceduresmentioning

confidence: 99%

The relation between cartilage damage and osteophyte size in a murine model for osteoarthritis in the knee

et al. 1996

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The aim of this study was to investigate the relationship between location and size of osteophytes and cartilage loss in an instability-induced experimental model for osteoarthritis. Osteoarthritis was induced in murine knee joints by injection of highly purified bacterial collagenase, causing joint instability. The size of the osteophytes and the cartilage loss were measured at different locations in the joint using image analysis on histological sections of total kees. Cartilage damage did not occur without osteophytes. Osteophytes were located on both medial and lateral sides, independent of the location of cartilage damage, but the size of the osteophytes was related to the amount of cartilage damage on the corresponding side. Cartilage loss on the lateral tibial plateau correlated well with the size of lateral osteophytes, in particular with the osteophyte at the margin of the lateral tibial plateau. Cartilage loss on the medial tibial plateau appeared to have a good correlation with the size of medial osteophytes, which was most pronounced for the osteophyte on the medial margin of the tibial plateau. This side-specific correlation between cartilage damage and osteophyte formation suggests compartmentalization of the osteoarthritic process.

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Musculoskeletal system

Scudamore¹

2013

A Practical Guide to the Histology of the Mouse

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Quantification of morphometric changes in murine experimental osteoarthritis using image analysis

Cited by 9 publications

References 19 publications

A Comprehensive Histological Assessment of Osteoarthritis Lesions in Mice

A Comprehensive Histological Assessment of Osteoarthritis Lesions in Mice

The relation between cartilage damage and osteophyte size in a murine model for osteoarthritis in the knee

Musculoskeletal system

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