Quantification of Nitrite/Nitrate in Food Stuff Samples Using 2-Aminobenzoic Acid as a New Amine in Diazocoupling Reaction

Malingappa, Pandurangappa; Venkataramanappa, Yarradoddappa

doi:10.1007/s12161-010-9138-4

Cited by 17 publications

(3 citation statements)

References 22 publications

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“…Stoikiometri antara PABA dan NEDA tercapai pada fraksi mol NEDA 0,5 atau saat perbandingan mol PABA dan NEDA yaitu 1 : 1 (Gambar 3). Mekanisme reaksi diazotasi menggunakan PABA dan NEDA berdasarkan perbandingan mol yang diperoleh disajikan pada Gambar 4 (Tsikas, 2007;Pandurangappa dan Venkataramanappa, 2011).…”

Section: Hasil Dan Pembahasanunclassified

MONITORING KADAR NITRIT DAN NITRAT PADA AIR SUMUR DI DAERAH CATUR TUNGGAL YOGYAKARTA DENGAN METODE SPEKTROFOTOMETRI UV-VIS (Monitoring of Nitrite and Nitrate Content in Ground Water of Catur Tunggal Region of Yogyakarta by UV-VIS Spectrophotometry)

Setiowati¹,

Roto²,

Wahyuni³

2016

J. Man & Ling.

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AbstrakMetode analisis nitrit dan nitrat perlu dikembangkan untuk memonitor kualitas air minum. Kualitas air sumur untuk parameter nitrit dan nitrat dipengaruhi oleh kondisi lingkungan dan kedalaman air sumur. Penelitian ini bertujuan menganalisis nitrit dan nitrat menggunakan asam p-aminobenzoat (PABA) pada air sumur di daerah perkotaan Yogyakarta. Analisis nitrit didasarkan pada reaksi antara ion nitrit dengan PABA yang membentuk senyawa azo dengan panjang gelombang maksimum 546 nm. Kedalaman air sumur di daerah Catur Tunggal rata-rata > 10 m. Kadar nitrit dan nitrat pada air sumur adalah 0,05-0,09 dan 8,22-36,58 mg/L. Kadar nitrit dan nitrat tersebut memenuhi baku mutu dan aman untuk dikonsumsi. Konsentrasi nitrit dan nitrat pada air RO adalah 0,05 dan 2,72-59,57 mg/L. Kadar nitrit pada air RO tidak memenuhi baku mutu sedangkan kadar nitrat memenuhi baku mutu kecuali RO 5.Kata kunci: air sumur, asam p-aminobenzoat, kualitas air minum, nitrat, nitrit. AbstractThe method for analysis nitrite and nitrate had to developed to monitor the drinking water quality. PENDAHULUANPeningkatan jumlah penduduk perkotaan akan meningkatkan kebutuhan air bersih. Salah satu sumber air bersih adalah air tanah. Peranan air tanah semakin lama semakin penting karena air tanah menjadi sumber utama air untuk memenuhi kebutuhan pokok hajat hidup orang banyak seperti air minum, rumah tangga, industri, irigasi, perkotaan, dan lain-lain (Zeffitni, 2012). Salah satu pemanfaatan air tanah sebagai sumber air minum adalah penggunaan air sumur. Oleh karena itu, air sumur harus memenuhi parameter kualitas air minum seperti kandungan nitrit dan nitrat.Senyawa nitrogen (nitrit, nitrat dan amonia) di perairan secara alami berasal dari metabolisme organisme perairan dan dekomposisi bahan-bahan organik oleh bakteri (Indrayani dkk., 2015). Selain itu, nitrit dan nitrat di alam dapat dihasilkan secara alami maupun dari aktivitas manusia. Sumber alami nitrit dan nitrat adalah siklus nitrogen sedangkan sumber dari aktivitas manusia berasal dari penggunaan pupuk nitrogen, limbah industri dan limbah organik manusia. Pembentukan nitrit dan nitrat pada siklus nitrogen terjadi melalui proses fiksasi nitrogen oleh bakteri Rhizobium, nitrifikasi dan dinitrifikasi oleh bakteri Pseudomonas denitrifican. Nitritifikasi melibatkan dua proses yaitu nitritasi oleh bakteri Nitrosomonas dan nitratasi oleh bakteri Nitrobacter. Pada kondisi anaerob, nitrat adalah bentuk nitrogen yang cukup stabil tetapi dapat direduksi menjadi nitrit melalui proses nitratasi (Rosca dkk., 2009).

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Section: Hasil Dan Pembahasanunclassified

MONITORING KADAR NITRIT DAN NITRAT PADA AIR SUMUR DI DAERAH CATUR TUNGGAL YOGYAKARTA DENGAN METODE SPEKTROFOTOMETRI UV-VIS (Monitoring of Nitrite and Nitrate Content in Ground Water of Catur Tunggal Region of Yogyakarta by UV-VIS Spectrophotometry)

Setiowati¹,

Roto²,

Wahyuni³

2016

J. Man & Ling.

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“…The detection limit for peroxynitrous acid/nitrite (S/N = 3) was 0.6 μM. However, the standard spectrophotometric methods showed that the linear range for nitrite is 1.2−5.8 μM and the detection limit was ∼1.0 μM, 42,43 indicating that the proposed method exhibited a high sensitivity and a wide dynamic range. The relative standard deviation (RSD) for nine repeated measurements of 0.01 mM peroxynitrous acid/nitrite was 2.5%.…”

Section: ■ Results and Discussionmentioning

confidence: 89%

Universal Chemiluminescence Flow-Through Device Based on Directed Self-Assembly of Solid-State Organic Chromophores on Layered Double Hydroxide Matrix

Wang

Teng

2013

Anal. Chem.

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In this work, a universal chemiluminescence (CL) flow-through device suitable for various CL resonance energy transfer (CRET) systems has been successfully fabricated. Highly efficient CRET in solid-state photoactive organic molecules can be achieved by assembling them on the surface of layered double hydroxides (LDHs). We attribute these observations to the suppression of the intermolecular π-π stacking interactions among aromatic rings and the improvement of molecular orientation and planarity in the LDH matrix, enabling a remarkable increase in fluorescence lifetime and quantum yield of organic molecules. Under optimal conditions, using peroxynitrous acid-fluorescein dianion (FLUD) as a model CRET system, trace FLUD (10 μM) was assembled on the surface of LDHs. Peroxynitrous acid/nitrite could be assayed in the range of 1.0-500 μM, and the detection limit for peroxynitrous acid/nitrite (S/N = 3) was 0.6 μM. This CL flow-through device exhibited operational stability, high reproducibility, and long lifetime. While LDHs were immobilized in a flow-through device in the absence of FLUD, the detection limit for peroxynitrous acid/nitrite was 100 μM. On the other hand, FLUD at the same concentration can not enhance the CL intensity of peroxynitrous acid system. This fabricated CL flow-through column has been successfully applied to determine nitrite in sausage samples with recoveries of 98-102%. These satisfactory results demonstrated that our studies pave a novel way toward flow-through column-based CRET using solid-state organic molecules as acceptors for signal amplification.

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“…). NED has been long used for the chromogenic derivatization of compounds having nitrogen atoms . Until now, NED has only been used as an internal standard in CE .…”

Section: Introductionmentioning

confidence: 99%

Determination of peimine and peiminine in Bulbus Fritillariae Thunbergii by capillary electrophoresis by indirect UV detection using N‐(1‐naphthyl)ethylenediamine dihydrochloride as probe

et al. 2012

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A simple and inexpensive CE method was developed for the determination of peimine and peiminine. Because of the lack of an UV chromophore of peimine and peiminine, the detection method chosen was indirect UV detection, with N-(1-naphthyl)ethylenediamine dihydrochloride (NED) as the UV absorbing probe. It was thought that NED, a chromophoric ion, may form hydrogen bonding pairs with the analytes to cause significant changes in separation selectivity. Additionally, the hydrophobic interactions between analytes and the probe also play a crucial role in achieving a resolution between the two analytes. The analyses were carried out with a background electrolyte composed of 66% MeOH-ACN (1:1, v/v), 34% aqueous buffer containing 15 mM NaH₂PO₄, 2.5 mM NED, 4 mM H₃PO₄. MeOH-ACN mixtures used as organic modifiers can not only reduce the adsorption of NED to the capillary wall, but also decrease the baseline noise and drift. The method provided a linear response ranging from 5 to 200 μg/mL. The limits of detection (LODs) for peimine and peiminine were 3.9 and 4.1 μg/mL, respectively. The repeatabilities (n = 3) reached relative standard deviation values (RSDs) of 3.4 and 4.1% for the peak areas, 4.0 and 4.4% for the peak heights, and 0.29 and 0.30% for the migration time of peimine and peiminine, respectively. Regression equations revealed linear relationships (r = 0.9995-0.9996) between the peak area of each analyte and the concentration. The method developed was successfully applied to quantify peimine and peiminine in chloroform extracts of the ground Bulbus Fritillariae Thunbergii.

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Quantification of Nitrite/Nitrate in Food Stuff Samples Using 2-Aminobenzoic Acid as a New Amine in Diazocoupling Reaction

Cited by 17 publications

References 22 publications

MONITORING KADAR NITRIT DAN NITRAT PADA AIR SUMUR DI DAERAH CATUR TUNGGAL YOGYAKARTA DENGAN METODE SPEKTROFOTOMETRI UV-VIS (Monitoring of Nitrite and Nitrate Content in Ground Water of Catur Tunggal Region of Yogyakarta by UV-VIS Spectrophotometry)

MONITORING KADAR NITRIT DAN NITRAT PADA AIR SUMUR DI DAERAH CATUR TUNGGAL YOGYAKARTA DENGAN METODE SPEKTROFOTOMETRI UV-VIS (Monitoring of Nitrite and Nitrate Content in Ground Water of Catur Tunggal Region of Yogyakarta by UV-VIS Spectrophotometry)

Universal Chemiluminescence Flow-Through Device Based on Directed Self-Assembly of Solid-State Organic Chromophores on Layered Double Hydroxide Matrix

Determination of peimine and peiminine in Bulbus Fritillariae Thunbergii by capillary electrophoresis by indirect UV detection using N‐(1‐naphthyl)ethylenediamine dihydrochloride as probe

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