Quantification of phosphatidylserine, phosphatidic acid and free fatty acids in an ultrasound contrast agent by normal-phase high-performance liquid chromatography with evaporative light scattering detection

Hvattum, Erlend; Uran, Steinar; Sandbæk, Anne Gunvor; Karlsson, Anders; Skotland, Tore

doi:10.1016/j.jpba.2006.04.027

Cited by 28 publications

(12 citation statements)

References 38 publications

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“…given, and it is unclear whether the authors achieved efficient separation of PA from the other phospholipids, as shown in previous studies ( 76,77 ). This separation would be essential because we have demonstrated an increase in PS and PI mass in the fl d hearts.…”

Section: Examination Of the Downstream Signaling Effects Of Aberrant mentioning

confidence: 83%

Relationship of glucose and oleate metabolism to cardiac function in lipin-1 deficient (fld) mice

Kok

Kienesberger

Dyck

et al. 2012

Journal of Lipid Research

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of cardiac ATP production ( 1 ). FAs are also incorporated into triacylglycerol (TG), which is stored in lipid droplets. Cardiac TG turnover is rapid ( 2 ) and FAs released from TG can contribute up to 10-20% of ATP production ( 3 ). Plasma FA and TG levels rise in insulin resistance and diabetes ( 4, 5 ), and cardiac FA utilization can increase to supply as much as 90% of ATP ( 6, 7 ). Despite this increase in FAO, FA uptake can exceed the ability of the heart to utilize FAs, leading to excess accumulation of TG in cardiomyocytes ( 6-9 ). The compensatory changes that occur in response to excessive FA availability eventually become maladaptive, and the heart is locked in a pathological and infl exible metabolic state that contributes to cardiac dysfunction ( 6, 7, 10 ). As such, maintenance of metabolic fl exibility is essential for normal cardiac function. Whereas many proteins have been identifi ed to contribute to cardiac FA uptake and metabolism ( 1, 10 ), relatively little information is available about the role of lipin-1 in the heart. Lipin-1 is an important protein involved in regulating both TG synthesis and FAO in other organs ( 11 ).Instead of being considered as mutually antagonistic processes, FAO and TG synthesis are now widely viewed as being companion pathways because augmenting cardiac TG synthesis increases FAO ( 12 ). Moreover, FAs released through lipolysis of endogenous TG stores contribute signifi cantly to FAO ( 3,13 ). Lipin-1 could play a prominent role in this regulation as it is a bifunctional protein involved in regulating both TG synthesis and FAO in the The constant requirement for ATP in the beating heart is satisfi ed by the utilization of diverse substrates, such as fatty acids (FA), glucose, lactate, ketones, and amino acids, with FA oxidation (FAO) providing the majority (50-75%)

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Section: Examination Of the Downstream Signaling Effects Of Aberrant mentioning

confidence: 83%

Relationship of glucose and oleate metabolism to cardiac function in lipin-1 deficient (fld) mice

Kok

Kienesberger

Dyck

et al. 2012

Journal of Lipid Research

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“…The drug product consists of an aqueous dispersion of lipid-stabilised perfluorobutane (PFB)-filled gas microbubbles with median volume diameter of approximately 3 m (Sontum et al 1999). The microbubbles are stabilised with a monolayer of phospholipids obtained from hydrogenated egg phosphatidylserine, with palmitoyl-stearoyl-phosphatidylserine as the main constituent (Hvattum et al 2006). The currently approved dose for liver imaging in Japan is 0.12 L microbubbles/kg body weight (b.w.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics of Perfluorobutane Following Intravenous Bolus Injection and Continuous Infusion of Sonazoid™ in Healthy Volunteers and in Patients with Reduced Pulmonary Diffusing Capacity

Landmark

Johansen

Johnson³

et al. 2008

Ultrasound in Medicine & Biology

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“…However, high-performance liquid chromatography indicates that Sonazoid has the longest fatty acid hydrocarbon length of any agent with 70% being stearic acid (C18:0) or longer. 21 …”

Section: Discussionmentioning

confidence: 99%

Rest-Stress Limb Perfusion Imaging in Humans with Contrast Ultrasound Using Intermediate-Power Imaging and Microbubbles Resistant to Inertial Cavitation

Davidson

Hodovan

Belcik

et al. 2017

Journal of the American Society of Echocardiography

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Background Contrast enhanced ultrasound (CEU) limb perfusion imaging is a promising approach for for evaluating peripheral artery disease (PAD). However, low signal enhancement in skeletal muscle has necessitated high-power intermittent imaging algorithms which are not clinically feasible. We hypothesized that CEU using a combination of intermediate power and a contrast agent resistant to inertial cavitation would allow real-time limb stress perfusion imaging. Methods In normal volunteers, CEU of the calf skeletal muscle was performed on separate days with Sonazoid, Optison, or Definity. Progressive reduction in the ultrasound pulsing interval (PI) was used to assess the balance between signal enhancement and agent destruction at escalating mechanical indices (MI 0.1 to 0.4). Real-time perfusion imaging at MI 0.1 to 0.4 using post-destructive replenishment kinetics was performed at rest and during 25 W plantar flexion contractile exercise. Results For Optison, limb perfusion imaging was unreliable at rest due to very low signal enhancement generated at all MIs, and was possible during exercise-induced hyperemia only at MI 0.1 due to agent destruction at higher MIs. For Definity, signal intensity progressively increased with MI, but was offset by microbubble destruction which resulted in modest signal enhancement during CEU perfusion imaging and distortion of replenishment curves at MI ≥0.2. For Sonazoid, there strong signal enhancement at MI ≥0.2 with little destruction detected only at MI 0.4. Accordingly, high signal intensity and non-distorted perfusion imaging was possible at MI 0.2–0.3 and detected an 8.0±5.7-fold flow reserve. Conclusions Rest-stress limb perfusion imaging in humans with real-time CEU, which requires only seconds to perform, is possible using microbubbles with viscoelastic properties that produce strong non-linear signal generation without destruction at intermediate acoustic pressures.

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Quantification of phosphatidylserine, phosphatidic acid and free fatty acids in an ultrasound contrast agent by normal-phase high-performance liquid chromatography with evaporative light scattering detection

Cited by 28 publications

References 38 publications

Relationship of glucose and oleate metabolism to cardiac function in lipin-1 deficient (fld) mice

Relationship of glucose and oleate metabolism to cardiac function in lipin-1 deficient (fld) mice

Pharmacokinetics of Perfluorobutane Following Intravenous Bolus Injection and Continuous Infusion of Sonazoid™ in Healthy Volunteers and in Patients with Reduced Pulmonary Diffusing Capacity

Rest-Stress Limb Perfusion Imaging in Humans with Contrast Ultrasound Using Intermediate-Power Imaging and Microbubbles Resistant to Inertial Cavitation

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