2005
DOI: 10.1081/abio-200053404
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Quantification of Prion Gene Expression in Brain and Peripheral Organs of Golden Hamster by Real-Time Rt-PCR

Abstract: Determination of tissue-specific expression of cellular prion protein (PrPc) is essential for understanding its poorly explained role in organisms. Herein we report on quantification of PrP mRNA in golden hamsters, a popular experimental model for studying mechanisms of transmissible spongiform encephalopathies (TSE), by real-time RT-PCR. Total RNA was isolated from four different regions of the brain and six peripheral organs of eight golden hamsters. PrP mRNA copy numbers were determined using absolute stand… Show more

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Cited by 20 publications
(10 citation statements)
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“…The above tissue-specific PrP mRNA expression pattern in sheep is similar to works of Ales et al [9] and Ning et al [10] who found high PrP expression in neuronal tissues and highest PrP expression in Lymph node in peripheral organs in bovine and Golden Hamster respectively using similar real-time RT-PCR technique, and is also consistent with works of Atsutaka et al [2] who detected high PrP expression in brain of sheep and works of Harris et al [15,16] who measured chicken PrP mRNA in brain and other organs by in situ hybridization and Northern Blot. The high levels of expression of PrP in CNS across the species examined indicate its significant physiological role in neuronal tissues and are consistent with the involvement of CNS in TSE, whereas high PrP expression in lymphatic organs is consistent with the role of PrP c within immune system that PrP participates in lymphocyte activation [17,18].…”
Section: Discussionsupporting
confidence: 87%
See 1 more Smart Citation
“…The above tissue-specific PrP mRNA expression pattern in sheep is similar to works of Ales et al [9] and Ning et al [10] who found high PrP expression in neuronal tissues and highest PrP expression in Lymph node in peripheral organs in bovine and Golden Hamster respectively using similar real-time RT-PCR technique, and is also consistent with works of Atsutaka et al [2] who detected high PrP expression in brain of sheep and works of Harris et al [15,16] who measured chicken PrP mRNA in brain and other organs by in situ hybridization and Northern Blot. The high levels of expression of PrP in CNS across the species examined indicate its significant physiological role in neuronal tissues and are consistent with the involvement of CNS in TSE, whereas high PrP expression in lymphatic organs is consistent with the role of PrP c within immune system that PrP participates in lymphocyte activation [17,18].…”
Section: Discussionsupporting
confidence: 87%
“…The present study quantified for the first time the expression of PrP mRNA in CNS and peripheral organs of sheep using real-time quantitative RT-PCR with the fluorescent dye SYBR Green I [10]. Real-time PCR is an extremely sensitive technique for quantification of the target nucleotides [11,12].…”
Section: Discussionmentioning
confidence: 99%
“…In golden hamsters, PrP gene was reported to express in the brain and peripheral organs such as heart, liver, kidney and lung (14). In our work, we observed apoptosis induced by the expressed PrP glycosylation mutants in epithelial-derived (HeLa) cell lines in vitro.…”
Section: Introductionsupporting
confidence: 53%
“…In peripheral organs examined, inguinal lymph node showed a high level of expression similar to that of the overall brain; spleen and lung showed moderate levels of the expression (14). In our study, we had observed similar cell apoptosis induced by PrP mutants in both neuron-(SF126) (31) and epithelial-derived cell lines (HeLa), what is interesting is that non-glycosylated modified PrP was found to be much easier in triggering the apoptosis than the mono-glycosylated modified ones in PS inverting and PI staining analysis in both cells.…”
Section: Discussionmentioning
confidence: 96%
“…Taking into account that the presence of PrP C is essential to develop scrapie, many works have been done on the expression of PrP in central nervous system (CNS) and lymphoid tissues. The high levels of PrP mRNA expression in CNS and lymphoid tissues have been revealed by studies in CNS and some peripheral organs of sheep [6], in neuronal tissues and lymph node of bovine [7] and Golden Hamster [8], in brain of sheep [2], in brain and other organs of chicken [9,10]. In oral infection, it is important to know what happens when the agent gains access to the gastrointestinal tract and which pathways are exploited by the agent to spread throughout the body and attain the central nervous system (CNS).…”
Section: Introductionmentioning
confidence: 96%